Makoto Miyazaki, PhD

Publications

• Lipotoxicity in the pathogenesis of chronic kidney disease complications
• Miyazaki-Anzai S, Masuda M, Keenan AL, Shiozaki Y, Miyazaki M. Activation of the IKK2-NF?B pathway in VSMCs inhibits calcified vascular stiffness in CKD by reducing the secretion of calcifying extracellular vesicles. bioRxiv. 2023 Jul 12. PubMed PMID: 37502894
• Miyazaki-Anzai S, Keenan AL, Blaine J, Miyazaki M. Targeted Disruption of a Proximal Tubule-Specific TMEM174 Gene in Mice Causes Hyperphosphatemia and Vascular Calcification. J Am Soc Nephrol. 2022 Aug;33(8):1477-1486. PubMed PMID: 35459732
• Masuda M, Yoshida-Shimizu R, Mori Y, Ohnishi K, Adachi Y, Sakai M, Kabutoya S, Ohminami H, Yamanaka-Okumura H, Yamamoto H, Miyazaki M, Taketani Y. Sulforaphane induces lipophagy through the activation of AMPK-mTOR-ULK1 pathway signaling in adipocytes. J Nutr Biochem. 2022 Aug;106:109017. PubMed PMID: 35461903
• Yimamu Y, Ohtani A, Takei Y, Furuichi A, Kamei Y, Yamanaka-Okumura H, Ohminami H, Masuda M, Miyazaki M, Yamamoto H, Taketani Y. 25-hydroxyvitamin D-1a-hydroxylase (CYP27B1) induces ectopic calcification. J Clin Biochem Nutr. 2022 Sep;71(2):103-111. PubMed PMID: 36213783
• Shiozaki Y, Miyazaki-Anzai S, Keenan AL, Miyazaki M. MEF2D-NR4A1-FAM134B2-mediated reticulophagy contributes to amino acid homeostasis. Autophagy. 2021 Sep 14;:1-13. [Epub ahead of print] PubMed PMID: 34517786
• Garcia GE, Lu YJ, Truong LD, Roncal-Jiménez CA, Miyazaki M, Miyazaki-Anzai S, Cara-Fuentes G, Andres-Hernando A, Lanaspa M, Johnson RJ, Leamon CP. A Novel Treatment for Glomerular Disease: Targeting the Activated Macrophage Folate Receptor with a Trojan Horse Therapy in Rats. Cells. 2021 Aug 17;10(8). PubMed PMID: 34440885
• Miyazaki-Anzai S, Masuda M, Shiozaki Y, Keenan AL, Chonchol M, Kremoser C, Miyazaki M. Free Deoxycholic Acid Exacerbates Vascular Calcification in CKD through ER Stress-Mediated ATF4 Activation. Kidney360. 2021 May;2(5):857-868. PubMed PMID: 34423309
• Klionsky DJ, Abdel-Aziz AK, Abdelfatah S, Abdellatif M, Abdoli A, Abel S, Abeliovich H, Abildgaard MH, Abudu YP, Acevedo-Arozena A, Adamopoulos IE, Adeli K, Adolph TE, Adornetto A, Aflaki E, Agam G, Agarwal A, Aggarwal BB, Agnello M, Agostinis P, Agrewala JN, Agrotis A, Aguilar PV, Ahmad ST, Ahmed ZM, Ahumada-Castro U, Aits S, Aizawa S, Akkoc Y, Akoumianaki T, Akpinar HA, Al-Abd AM, Al-Akra L, Al-Gharaibeh A, Alaoui-Jamali MA, Alberti S, Alcocer-Gómez E, Alessandri C, Ali M, Alim Al-Bari MA, Aliwaini S, Alizadeh J, Almacellas E, Almasan A, Alonso A, Alonso GD, Altan-Bonnet N, Altieri DC, Álvarez ÉMC, Alves S, Alves da Costa C, Alzaharna MM, Amadio M, Amantini C, Amaral C, Ambrosio S, Amer AO, Ammanathan V, An Z, Andersen SU, Andrabi SA, Andrade-Silva M, Andres AM, Angelini S, Ann D, Anozie UC, Ansari MY, Antas P, Antebi A, Antón Z, Anwar T, Apetoh L, Apostolova N, Araki T, Araki Y, Arasaki K, Araújo WL, Araya J, Arden C, Arévalo MA, Arguelles S, Arias E, Arikkath J, Arimoto H, Ariosa AR, Armstrong-James D, Arnauné-Pelloquin L, Aroca A, Arroyo DS, Arsov I, Artero R, Asaro DML, Aschner M, Ashrafizadeh M, Ashur-Fabian O, Atanasov AG, Au AK, Auberger P, Auner HW, Aurelian L, Autelli R, Avagliano L, Ávalos Y, Aveic S, Aveleira CA, Avin-Wittenberg T, Aydin Y, Ayton S, Ayyadevara S, Azzopardi M, Baba M, Backer JM, Backues SK, Bae DH, Bae ON, Bae SH, Baehrecke EH, Baek A, Baek SH, Baek SH, Bagetta G, Bagniewska-Zadworna A, Bai H, Bai J, Bai X, Bai Y, Bairagi N, Baksi S, Balbi T, Baldari CT, Balduini W, Ballabio A, Ballester M, Balazadeh S, Balzan R, Bandopadhyay R, Banerjee S, Banerjee S, Bánréti Á, Bao Y, Baptista MS, Baracca A, Barbati C, Bargiela A, Barilà D, Barlow PG, Barmada SJ, Barreiro E, Barreto GE, Bartek J, Bartel B, Bartolome A, Barve GR, Basagoudanavar SH, Bassham DC, Bast RC Jr, Basu A, Batoko H, Batten I, Baulieu EE, Baumgarner BL, Bayry J, Beale R, Beau I, Beaumatin F, Bechara LRG, Beck GR Jr, Beers MF, Begun J, Behrends C, Behrens GMN, Bei R, Bejarano ... Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)(1). Autophagy. 2021 Jan;17(1):1-382. PubMed PMID: 33634751
• Shiozaki Y, Miyazaki-Anzai S, Okamura K, Keenan AL, Masuda M, Miyazaki M. GPAT4-Generated Saturated LPAs Induce Lipotoxicity through Inhibition of Autophagy by Abnormal Formation of Omegasomes. iScience. 2020 May 22;23(5):101105. PubMed PMID: 32408172
• Niida Y, Masuda M, Adachi Y, Yoshizawa A, Ohminami H, Mori Y, Ohnishi K, Yamanaka-Okumura H, Uchida T, Nikawa T, Yamamoto H, Miyazaki M, Taketani Y. Reduction of stearoyl-CoA desaturase (SCD) contributes muscle atrophy through the excess endoplasmic reticulum stress in chronic kidney disease. J Clin Biochem Nutr. 2020 Sep;67(2):179-187. PubMed PMID: 33041516
• Masuda M, Yamamoto H, Takei Y, Nakahashi O, Adachi Y, Ohnishi K, Ohminami H, Yamanaka-Okumura H, Sakaue H, Miyazaki M, Takeda E, Taketani Y. All-trans retinoic acid reduces the transcriptional regulation of intestinal sodium-dependent phosphate co-transporter gene (Npt2b). Biochem J. 2020 Feb 28;477(4):817-831. PubMed PMID: 32016357
• Brown CN, Atwood DJ, Pokhrel D, Ravichandran K, Holditch SJ, Saxena S, Miyazaki M, Nemenoff R, Weiser-Evans MCM, Ljubanovic DG, Joy MS, Edelstein CL. The effect of MEK1/2 inhibitors on cisplatin-induced acute kidney injury (AKI) and cancer growth in mice. Cell Signal. 2020 Jul;71:109605. PubMed PMID: 32194168
• Kohno S, Keenan AL, Ntambi JM, Miyazaki M. Lipidomic insight into cardiovascular diseases. Biochem Biophys Res Commun. 2018 Oct 7;504(3):590-595. PubMed PMID: 29665359
• Miyazaki-Anzai S, Masuda M, Kohno S, Levi M, Shiozaki Y, Keenan AL, Miyazaki M. Simultaneous inhibition of FXR and TGR5 exacerbates atherosclerotic formation. J Lipid Res. 2018 Sep;59(9):1709-1713. PubMed PMID: 29976576
• Shiozaki Y, Okamura K, Kohno S, Keenan AL, Williams K, Zhao X, Chick WS, Miyazaki-Anzai S, Miyazaki M. The CDK9-cyclin T1 complex mediates saturated fatty acid-induced vascular calcification by inducing expression of the transcription factor CHOP. J Biol Chem. 2018 Nov 2;293(44):17008-17020. PubMed PMID: 30209133
• Kleczko EK, Marsh KH, Tyler LC, Furgeson SB, Bullock BL, Altmann CJ, Miyazaki M, Gitomer BY, Harris PC, Weiser-Evans MCM, Chonchol MB, Clambey ET, Nemenoff RA, Hopp K. CD8( ) T cells modulate autosomal dominant polycystic kidney disease progression. Kidney Int. 2018 Dec;94(6):1127-1140. PubMed PMID: 30249452
• Trostel J, Truong LD, Roncal-Jimenez C, Miyazaki M, Miyazaki-Anzai S, Kuwabara M, McMahan R, Andres-Hernando A, Sato Y, Jensen T, Lanaspa MA, Johnson RJ, Garcia GE. Different effects of global osteopontin and macrophage osteopontin in glomerular injury. Am J Physiol Renal Physiol. 2018 Oct 1;315(4):F759-F768. PubMed PMID: 29717936
• Ravichandran K, Holditch S, Brown CN, Wang Q, Ozkok A, Weiser-Evans MC, Nemenoff R, Miyazaki M, Thiessen-Philbrook H, Parikh CR, Ljubanovic D, Edelstein CL. IL-33 deficiency slows cancer growth but does not protect against cisplatin-induced AKI in mice with cancer. Am J Physiol Renal Physiol. 2018 Mar 1;314(3):F356-F366. PubMed PMID: 29070568
• Jovanovich A, Isakova T, Block G, Stubbs J, Smits G, Chonchol M, Miyazaki M. Deoxycholic Acid, a Metabolite of Circulating Bile Acids, and Coronary Artery Vascular Calcification in CKD. Am J Kidney Dis. 2018 Jan;71(1):27-34. PubMed PMID: 28801122
• Jovanovich A, Isakova T, Block G, Stubbs J, Smits G, Chonchol M, Miyazaki M. Deoxycholic Acid, a Metabolite of Circulating Bile Acids, and Coronary Artery Vascular Calcification in CKD. Am J Kidney Dis. 2018 Jan;71(1):27-34. PubMed PMID: 28801122
• Lipidmoic insight of cardiovascular diseases. Shohei Kohno, Audrey L. Keenan James M Ntambi Makoto Miyazaki. Biochem Biophys Res Comm
• Ravichandran K, Holditch S, Brown CN, Wang Q, Ozkok A, Weiser-Evans MC, Nemenoff RA, Miyazaki M, Thiessen-Philbrook H, Parikh CR, Ljubanovic D, Edelstein CL. IL-33 deficiency slows cancer growth but does not protect against cisplatin-induced AKI in mice with cancer. Am J Physiol Renal Physiol. 2017 Oct 25;:ajprenal.00040.2017. [Epub ahead of print] PubMed PMID: 29070568
• Masuda M, Miyazaki-Anzai S, Keenan AL, Shiozaki Y, Okamura K, Chick WS, Williams K, Zhao X, Rahman SM, Tintut Y, Adams CM, Miyazaki M. Activating transcription factor-4 promotes mineralization in vascular smooth muscle cells. JCI Insight. 2016 Nov 3;1(18):e88646. PubMed PMID: 27812542
• Rahman SM, Baquero KC, Choudhury M, Janssen RC, de la Houssaye BA, Sun M, Miyazaki-Anzai S, Wang S, Moustaid-Moussa N, Miyazaki M, Friedman JE. C/EBPß in bone marrow is essential for diet induced inflammation, cholesterol balance, and atherosclerosis. Atherosclerosis. 2016 Jul;250:172-9. PubMed PMID: 27072340
• Roncal-Jimenez CA, Milagres T, Andres-Hernando A, Kuwabara M, Jensen T, Song Z, Bjornstad P, Garcia GE, Sato Y, Sanchez-Lozada LG, Lanaspa MA, Johnson RJ. The effects of exogenous desmopressin on a model of heat stress nephropathy in mice. Am J Physiol Renal Physiol. 2016 Dec 21;:ajprenal.00495.2016. [Epub ahead of print] PubMed PMID: 28003190
• Fatty Acid Desaturation and Elongation in Mammals Laura M. Bond · Makoto Miyazaki · Lucas M. O’Neill · Fang Ding · James M. Ntambi · Biochemistry of Lipids, Lipoproteins and Membranes, 01/2016: pages 185-208; , ISBN: 9780444634382
• Masuda M, Miyazaki-Anzai S, Keenan AL, Okamura K, Kendrick J, Chonchol M, Offermanns S, Ntambi JM, Kuro-O M, Miyazaki M. Saturated phosphatidic acids mediate saturated fatty acid-induced vascular calcification and lipotoxicity. J Clin Invest. 2015 Oct 26;2015. PubMed PMID: 26517697
• Miyazaki-Anzai S, Masuda M, Levi M, Keenan AL, Miyazaki M. Dual activation of the bile acid nuclear receptor FXR and G-protein-coupled receptor TGR5 protects mice against atherosclerosis. PLoS One. 2014;9(9):e108270. PubMed PMID: 25237811
• Miyazaki-Anzai S, Masuda M, Demos-Davies KM, Keenan AL, Saunders SJ, Masuda R, Jablonski K, Cavasin MA, Kendrick J, Chonchol M, McKinsey TA, Levi M, Miyazaki M. Endoplasmic reticulum stress effector CCAAT/enhancer-binding protein homologous protein (CHOP) regulates chronic kidney disease-induced vascular calcification. Circulation. 2014 Jun 24;3(3):e000949. PubMed PMID: 24963104
• CCAAT/enhancer binding protein ß deletion increases mitochondrial function and protects mice from LXR-induced hepatic steatosis. Rahman SM, Choudhury M, Janssen RC, Baquero KC, Miyazaki M, Friedman JE. Biochem Biophys Res Commun. 2013 Jan 4;430(1):336-9
• Ting C Ting and Miyazaki M. Stearoyl-CoA desaturase in the regulation of cardiovascular disease and endoplasmic reticulum stress. Ntambi JM (Eds.), pp 73-84 (2013)
• Masuda M, Miyazaki-Anzai S, Levi M, Ting TC and Miyazaki M. PERK-eIF2?-ATF4-CHOP signaling contributes to TNF?-induced vascular calcification. J. Am. Heart Assoc., 2013, 2:e000238.
• Rahman SM, Choudhury M, Janssen RC, Baquero KC, Miyazaki M and Friedman JE. Loss of C/EBPß protected mice from LXR agonist (T0901317) mediated induction of liver TG accumulation and lipogenesis but increased liver mitochondrial function. Biochem. Biophys. Res. Commun., 2013, 430:336-339
• Roncal Jimenez CA, Ishimoto T, Lanaspa MA, Rivard CJ, Nakagawa T, Ejaz AA, Cicerchi C, Inaba S, Le M, Miyazaki M, Glaser J, Correa-Rotter R, González MA, Aragón A, Wesseling C, Sánchez-Lozada LG, Johnson RJ. Fructokinase activity mediates dehydration-induced renal injury. Kidney Int. 2013
• Masuda M, Miyazaki-Anzai S, Miyazaki MGPAT4 contributes to stearate-induced vascular calcification. Kern Lipid Conference. (Vail, CO)
• Masuda M, Miyazaki-Anzai S, Miyazaki MPERK-eIF2?-ATF4-CHOP signaling contributes to TNF?-induced vascular calcification. American Society for Bone and Mineral Research. (Baltimore, MD)
• Miyazaki M, Deoxycholic acid contributes to chronic kidney disease-dependent vascular calcification. American Heart Association. (Dallas, TX)
• T Stearoyl-CoA desaturase in the regulation of cardiovascular disease and endoplasmic reticulum stress. Ting TC, Miyazaki M. JM Ntambi (Eds) in press
• Synthetic farnesoid X receptor agonists induce high-density lipoprotein-mediated transhepatic cholesterol efflux in mice and monkeys and prevent atherosclerosis in cholesteryl ester transfer protein transgenic low-density lipoprotein receptor (-/-) mice. Hambruch E, Miyazaki-Anzai S, Hahn U, Matysik S, Boettcher A, Perovic-Ottstadt S, Schlüter T, Kinzel O, Krol HD, Deuschle U, Burnet M, Levi M, Schmitz G, Miyazaki M, J Pharmacol Exp Ther. 2012 Dec;343(3):556-67
• Epicatechin limits renal injury by mitochondrial protection in cisplatin nephropathy. Tanabe K, Tamura Y, Lanaspa MA, Miyazaki M, Suzuki N, Sato W, Maeshima Y, Schreiner GF, Villarreal FJ, Johnson RJ, Nakagawa T. Am J Physiol Renal Physiol. 2012 Nov 1;303(9):F1264-74
• Nicorandil as a novel therapy for advanced diabetic nephropathy in the eNOS deficient mouse. Tanabe K, Lanaspa MA, Kitagawa W, Rivard CJ, Miyazaki M, Klawitter J, Schreiner GF, Saleem MA, Mathieson PW, Makino H, Johnson RJ, Nakagawa T. Am J Physiol Renal Physiol. 2012 Feb 15.
• Activating transcription factor 4 regulates stearate-induced vascular calcification. Masuda M, Ting TC, Levi M, Saunders SJ, Miyazaki-Anzai S, Miyazaki M. J Lipid Res. 2012 Aug;53(8):1543-52.
• Increased lipogenesis and stearate accelerate vascular calcification in calcifying vascular cells. Ting TC, Miyazaki-Anzai S, Masuda M, Levi M, Demer LL, Tintut Y, Miyazaki M. J Biol Chem. 2011 Jul 8;286(27):23938-49.
• Role of cellular cholesterol metabolism in vascular cell calcification. Geng Y, Hsu JJ, Lu J, Ting TC, Miyazaki M, Demer LL, Tintut Y. J Biol Chem. 2011 Sep 23;286(38):33701-6.
• Identification of cholesterol crystals in plaques of atherosclerotic mice using hyperspectral CARS imaging. Lim RS, Suhalim JL, Miyazaki-Anzai S, Miyazaki M, Levi M, Potma EO, Tromberg BJ. J Lipid Res. 2011 Dec;52(12):2177-86.
• Miyazaki M, Role of bile acid signaling in the regulation of vasculat diseases. Steenbock symposium. Madison WI
• Miyazaki M, Activation of the Bile Acid G-coupled Receptor TGR5 Protects Against Atherosclerosis Kern Lipid conference. Vail CO
• Masashi Masuda, Tabitha C. Ting, Moshe Levi, Sommer J. Saunders, Shinobu Miyazaki-Anzai, and Makoto Miyazaki, Increased lipogenesis and stearate accelerate vascular calcification and endoplasmic reticulum stress in calcifying vascular cells.Vail CO
• Loss of Stearoyl-CoA desaturase-1 attenuates adipocyte inflammation: effects of adipocyte-derived oleate. Liu X, Miyazaki M, Flowers MT, Sampath H, Zhao M, Chu K, Paton CM, Joo DS, Ntambi JM. Arterioscler Thromb Vasc Biol. 2010 Jan;30(1):31-8. Epub 2009 Nov 12. PMID: 19910642 [PubMed - indexed for MEDLINE]
• Farnesoid X receptor activation prevents the development of vascular calcification in ApoE-/- mice with chronic kidney disease. Miyazaki-Anzai S, Levi M, Kratzer A, Ting TC, Lewis LB, Miyazaki M. Circ Res. 2010 Jun 25;106(12):1807-17. Epub 2010 Apr 29.
• Multimodal CARS microscopy determination of the impact of diet on macrophage infiltration and lipid accumulation on plaque formation in ApoE-deficient mice. Lim RS, Kratzer A, Barry NP, Miyazaki-Anzai S, Miyazaki M, Mantulin WW, Levi M, Potma EO, Tromberg BJ. J Lipid Res. 2010 Jul;51(7):1729-37. Epub 2010 Mar 5.
• 1. Kratzer A, Buchebner M, Pfeifer T, Becker TM, Uray G, Miyazaki M, Miyazaki-Anzai S, Ebner B, Chandak PG, Kadam RS, Calayir E, Rathke N, Ahammer H, Radovic B, Trauner M, Hoefler G, Kompella UB, Fauler G, Levi M, Levak-Frank S, Kostner GM, Kratky D. J. Lipid Res. 2008 in press
• Stearoyl-CoA desaturase-1 deficiency attenuates obesity and insulin resistance in leptin-resistant obese mice. Miyazaki M, Sampath H, Liu X, Flowers MT, Chu K, Dobrzyn A, Ntambi JM. Biochem Biophys Res Commun. 2009 Mar 20;380(4):818-22. Epub 2009 Feb 5.
• 2. Mar-Heyming R, Miyazaki M, Weissglas-Volkov D, Kolaitis NA, Sadaat N, Plaisier C, Pajukanta P, Cantor RM, de Bruin TW, Ntambi JM, Lusis AJ. Association of stearoyl-CoA desaturase 1 activity with familial combined hyperlipidemia.Arterioscler Thromb Vasc Biol. 2008 28:1193-1199.
• Related ArticlesChen C, Shah YM, Morimura K, Krausz KW, Miyazaki M, Richardson TA, Morgan ET, Ntambi JM, Idle JR, Gonzalez FJ.Metabolomics Reveals that Hepatic Stearoyl-CoA Desaturase 1 Downregulation Exacerbates Inflammation and Acute Colitis.
• Miyazaki M. Role of stearoyl-CoA desaturase-1 in regulation of energy metabolism. Protein, Nucleic Acid and Enzyme. 2008 53:1375-82
• Miyazaki M, Flowers MT, Sampath H, Chu K, Otzelberger C, Liu X, Ntambi JM.Hepatic stearoyl-CoA desaturase-1 deficiency protects mice from carbohydrate-induced adiposity and hepatic steatosis
• Related Articles, LinksDobrzyn P, Sampath H, Dobrzyn A, Miyazaki M, Ntambi JM.Loss of stearoyl-CoA desaturase 1 inhibits fatty acid oxidation and increases glucose utilization in the heart.
• 11. Miyazaki M and Ntambi JM. Fatty acid desaturation and chain elongation in mammals, Biochemistry of Lipids. Lipoproteins and Membranes, 6th edition. D. E. Vance and J.E. Vance (Eds.), in press
• C/EBPbeta in bone marrow is essential for diet induced inflammation, cholesterol balance, and atherosclerosis. KC Baquero; M Choudhury; RC Janssen; Becky A de la Houssaye; M Sun; SS Miyazaki-Anzai; SWang; N Moustaid-Moussa; M Miyazaki; JE Friedman. Atherosclerosis. accepted
• Intranasal Administration of Sugarcane Ash Causes Chronic Kidney Disease in Rats AJP-Renal

Practice Locations

UCHealth University of Colorado Hospital (UCH)
12605 E. 16th Ave
Aurora, CO 80045
720-848-0000

Specialty Information

Specialties

• Nephrology