Daniel Ambruso, MD

Research Interests

I am interested in Neutrophil Function in the Following Clinical Groups: Thermal Injury and Trauma Patients; Newborn Patients; Patients with Absence of Specific Granules; Patients with Glycogenosis lb; and Patients with Chronic Granulomatous Disease. I, also, focus on Chronic Transfusion Therapy and Iron Overload in Patients with Chronic Anemia. Additionally, I am interested in Antibody-Mediated Disorders of Platelets and Neutrophils, as well as the Generation of Lysophosphatidylcholines in Stored Blood Components and their relationship to the Pathophysiology of Transfusion Related Acute Lung Injury (TRALI).

Publications

• Ambruso DR, Ellison MA, Thurman GW, Leto TL. Peroxiredoxin 6 translocates to the plasma membrane during neutrophil activation and is required for optimal NADPH oxidase activity. Biochim Biophys Acta. 2012 Feb;1823(2):306-15. PubMed PMID: 22178385
• Knipstein JA, Ambruso DR. Sweet syndrome in an infant with chronic granulomatous disease. J Pediatr Hematol Oncol. 2012 Jul;34(5):372-4. PubMed PMID: 22246153
• Ambruso DR, Sanchez R, Sloan SR, Josephson CD, the Pediatric TMAA Group. How do we teach pediatric topics in transfusion medicine: curriculum development, learners, and instructional strategies. Transfusion. 2013 Feb;53(2):250-256. PubMed PMID: 22519665
• Ellison MA, Thurman GW, Ambruso DR. Phox activity of differentiated PLB-985 cells is enhanced, in an agonist specific manner, by the PLA2 activity of Prdx6-PLA2. Eur J Immunol. 2012 Jun;42(6):1609-17. PubMed PMID: 22678913
• Reesink HW, Lee J, Keller A, Dennington P, Pink J, Holdsworth R, Schennach H, Goldman M, Petraszko T, Sun J, Meng Y, Qian K, Rehacek V, Turek P, Krusius T, Juvonen E, Tiberghien P, Legrand D, Semana G, Muller JY, Bux J, Reil A, Lin CK, Daly H, McSweeney E, Porretti L, Greppi N, Rebulla P, Okazaki H, Sánchez-Guerrero SA, Baptista-González HA, Martínez-Murillo C, Guerra-Márquez A, Rodriguez-Moyado H, Middelburg RA, Wiersum-Osselton JC, Brand A, van Tilburg C, Dinesh D, Dagger J, Dunn P, Brojer E, Letowska M, Maslanka K, Lachert E, Uhrynowska M, Zhiburt E, Palfi M, Berlin G, Frey BM, Puig Rovira L, Muñiz-Diaz E, Castro E, Chapman C, Green A, Massey E, Win N, Williamson L, Silliman CC, Chaffin DJ, Ambruso DR, Blumberg N, Tomasulo P, Land KJ, Norris PJ, Illoh OC, Davey RJ, Benjamin RJ, Eder AF, McLaughlin L, Kleinman S, Panzer S. Measures to prevent transfusion-related acute lung injury (TRALI). Vox Sang. 2012 Oct;103(3):231-59. PubMed PMID: 22519920
• Ellison MA, Ambruso DR, Silliman CC. Therapeutic options for transfusion related acute lung injury; the potential of the G2A receptor. Curr Pharm Des. 2012;18(22):3255-9. PubMed PMID: 22621271
• Silliman CC, Kelher M, Ambruso DR. Bioactive lipids from stored cellular blood components: in vitro method is crucial for proper interpretation. Transfusion. 2012 May;52(5):1155-7; author reply 1157-8. PubMed PMID: 22571364
• Ambruso DR, Hays T, Goldenberg N, Nuss R: Hematologic Disorders. In Current Pediatric Diagnosis and Treatment, WW Hay Jr, MJ Levin, JM Sondheimer, RR Deterding (eds), Appleton & Lange, (2012, 21st edition).
• Ambruso DR, Johnston RB Jr: Chronic Granulomatous Disease of Childhood and Common Variable Immunodeficiency. In Kendig's Disorder of the Respiratory Tract in Chidren, Eighth Edition. V Chernick, TF Boat (eds), WB Saunders Co., 2012, pp 886-898.
• Ambruso DR, Ellison M, Thurman G. Decreased Bactericidal Activity and Superoxide Anion (O2-) Production Associated with Mild Neutropenia: A Novel Neutrophil Dysfunction Syndrome. (Presented in platform session at PAS national meeting, Boston, MA, April 2012).
• Ambruso DR, Ellison M, Thurman G. Decreased Bactericidal Activity and Superoxide Anion (O2-) Production Associated with Mild Neutropenia: A Novel Neutrophil Dysfunction Syndrome. (Presented in platform session at PAS national meeting, Boston, MA, April 2012).
• Ambruso DR, Seewald R, Marshner S, Goodrich RP. Expression of Platelet (PLT) P-selectin and PLT-Neutrophil (PMN) Interaction after Treatment of Whole Blood with Mirasol PRT. (Presented at the 54th Annual meeting of the American Society For Hematology, New Orleans, LA December, 2012).
• Ambruso DR, Seewald R, DiPaola J, Brodsky G, Marschner S, Goodrich RP. Adherence Characteristics of Stored Apheresis Platelets (Plts) With and Without Pathogen Reduction Treatment (PRT) in a Microfluidic Flow Chamber. (Presented at the 54th Annual meeting of the American Society for Hematology, December, 2012, New Orleans, LA).
• Ellison MA, Ambruso DR, Thurman G. Peroxiredoxin 6 Enhances Phagocyte NADPH Oxidase (phox) Activity in Response to Agonists Acting via Cell Surface Receptors and Intracellular Signaling Pathways. (Presented at the 54th Annual Meeting of the American Society for Hematology, December, 2012, New Orleans, LA).
• Branchford BR, Scharer GH, Springer M, Gessner J, LeRoux J, Lunt B, Swisshelm K, Ambruso DR. T-lymphocyte immunodeficiency associated with chromosome 15q13.3 microdeletion.
• Josephson CD, Mondoro TH, Ambruso DR, Sanchez R, Sloan SR, Sola-Visner M, Manno CS, Silberstein LE, Goodrich RP, Strauss RG, Kirpalani H, Widness JA, Stramer S, Reed W, Hume HA, Luban NLC, Hillyer CD. One size will never fit all: the future of research in pediatric transfusion medicine.
• Dzieciatkowski M, Silliman CC, Moore EE, KelherMR, Banerjee A, Land KJ, Ellison M, Ambruso DR, Hansen, K. Proteomic Analysis of the Plasma Fraction of Packed Red Blood Cells : The Effects of Storage and Leukoreduction. Vox Sang (in review).
• Bercovitz RS, Macy M, Ambruso DR. A case of autoimmune hemolytic anemia with anti-D specificity in a 1-year-old child. Immunohematology. 2013;29(1):15-8. PubMed PMID: 24046918
• Ambruso DR. Peroxiredoxin-6 and NADPH oxidase activity. Methods Enzymol. 2013;527:145-67. PubMed PMID: 23830630
• Dzieciatkowska M, Silliman CC, Moore EE, Kelher MR, Banerjee A, Land KJ, Ellison M, West FB, Ambruso DR, Hansen KC. Proteomic analysis of the supernatant of red blood cell units: the effects of storage and leucoreduction. Vox Sang. 2013 Oct;105(3):210-8. PubMed PMID: 23663258
• Ambruso DR, Marschner S, Seewald R, Thurman G, Goodrich R. UV Irradiation of platelet concentrates open to air induces cell activation.
• Ambruso DR, Sanchez R, Sloan SR, Josephson CD, Pediatric TMAA Group. How do we teach pediatric topics in transfusion medicine: curriculum development, learners, and instructional strategies. Transfusion. 2013 Feb;53(2):250-6. PubMed PMID: 22519665
• Ambruso DR. Transfusion complications in thalassemias. Transfusion (in press)
• Ambruso DR. Hydroxyethyl starch and granulocyte transfusions: Considerations of utility and toxicity for patients and donors. Transfusion (accepted with revision)
• Josephson CD, Mondoro TH, Ambruso DR, Sanchez R, Sloan SR, Sola-Visner M, MannoCS, Silberstein LE, Goodrich RP, Strauss RG Kirpalani H, Widness JA, Stramer S, Reed W, Hume HA, Luban NLC, Hillyer CD. One size will never fit all: The future research in pediatric transfusion medicine. Pediatric Research (in press).
• Branchford BR, Scharer GH, Springer M, Gessner J, LeRoux J, Lunt B, Swisshelm K, Ambruso DR. T-lymphocyte immunodeficiency associated with chromasome 15q13.3 microdeletion. (submitted).
• Ambruso DR, Nuss R, Wang M. Hematologic disorders in Current Pediatrics Diagnosis and Treatment, 21 ed (in press)
• Ballen KK, Logan BR, Laughlin MJ, He W, Ambruso DR, Armitage SE, Beddard RL, Bhatla D, Hwang WY, Kiss JE, Koegler G, Kurtzberg J, Nagler A, Oh D, Petz LD, Price TH, Quinones RR, Ratanatharathorn V, Rizzo JD, Sazama K, Scaradavou A, Schuster MW, Sender LS, Shpall EJ, Spellman SR, Sutton M, Weitekamp LA, Wingard JR, Eapen M. Effect of Cord Blood Processing on Transplantation Outcomes after Single Myeloablative Umbilical Cord Blood Transplantation. Biol Blood Marrow Transplant. 2014 Dec 24. [Epub ahead of print] PubMed PMID: 25543094
• Cancelas JA, Padmanabhan A, Le T, Ambruso DR, Rugg N, Worsham DN, Pinkard SL, Graminske S, Buck J, Goldberg J, Bill J. Spectra Optia granulocyte apheresis collections result in higher collection efficiency of viable, functional neutrophils in a randomized, crossover, multicenter trial. Transfusion. 2014 Nov 10. [Epub ahead of print] PubMed PMID: 25382805
• Ambruso DR. Hydroxyethyl starch and granulocyte transfusions: considerations of utility and toxicity profile for patients and donors. Transfusion. 2014 Oct 15. [Epub ahead of print] PubMed PMID: 25315227
• Josephson CD, Mondoro TH, Ambruso DR, Sanchez R, Sloan SR, Luban NL, Widness JA. One size will never fit all: the future of research in pediatric transfusion medicine. Pediatr Res. 2014 Nov;76(5):425-31. PubMed PMID: 25119336
• Ambruso DR. Transfusion complications in thalassemias. Transfusion. 2014 Apr;54(4):957-9. PubMed PMID: 24724786
• McKinney CM, Siringo F, Olson JL, Capocelli KE, Ambruso DR, Nuss R. Red cell exchange transfusion halts progressive proliferative sickle cell retinopathy in a teenage patient with hemoglobin SC disease. Pediatr Blood and Cancer (in press)
• Maloney JP, Ambruso DR, Voelkel NF, Silliman CC. Platelet Vegef is a potential mediator of Transfusion-related acute lung injury. 2014 J Pulm Respir Med 4: 2012. doi:10.4172/2161-105X.1000212.
• Ambruso Dr, Nuss R, Wong M. Hematologic disorders. In WW Hay, MJ Levin, RR Deterding, and MJ Abzug eds. Current Diagnosis & Treatment Pediatrics. 23 edition. McGraw Hill Education, 2015.
• Zelinskaya A, Hillam M, Ambruso DR. Reducing access pressure alerts on Trima Accel. Transfusion 2014; 54 (Suppl): 130A.
• Ellison M, Thurman G, Ambruso DR. Peroxiredoxin 6 (Prdx6) is associated with change in autophagy in myeloid and non-myeloid cells 2014 Blood (Abstracts); 124: no. 1407.
• Ellison M, Thurman G, Ambruso DR. Myeloid cells differentiated in the presence of Interferon-gamma compared to mature cells exposed to this cytokine exhibit different phox protein expression and Nox2 activity 2014 Blood (Abstract);124: no.4103.
• Ellison MA, Thurman G, Gearheart CM, Seewald RH, Porter CC, Ambruso DR. INF-gamma Enhances Nox2 Activity by Upregulating phox Proteins When Applied to Differentiating PLB-985 Cells but Does Not Induce Nox2 Activity by Itself. PLoS One. 2015;10(8):e0136766. PubMed PMID: 26317224
• McKinney CM, Siringo F, Olson JL, Capocelli KE, Ambruso DR, Nuss R. Red cell exchange transfusion halts progressive proliferative sickle cell retinopathy in a teenaged patient with hemoglobin SC disease. Pediatr Blood Cancer. 2015 Apr;62(4):721-3. PubMed PMID: 25631233
• Ballen KK, Logan BR, Laughlin MJ, He W, Ambruso DR, Armitage SE, Beddard RL, Bhatla D, Hwang WY, Kiss JE, Koegler G, Kurtzberg J, Nagler A, Oh D, Petz LD, Price TH, Quinones RR, Ratanatharathorn V, Rizzo JD, Sazama K, Scaradavou A, Schuster MW, Sender LS, Shpall EJ, Spellman SR, Sutton M, Weitekamp LA, Wingard JR, Eapen M. Effect of cord blood processing on transplantation outcomes after single myeloablative umbilical cord blood transplantation. Biol Blood Marrow Transplant. 2015 Apr;21(4):688-95. PubMed PMID: 25543094
• Ambruso DR, Nuss R, Wong, M. Hematologic Disorders. In WW Hay, MJ Levin, RR Deterding and MJ Abzug eds. Current Diagnosis & Treatment in Pediatrics. 23 edition. McGraw Hill Education, 2015
• Cancelas JA, Padmanabhan A, Le T, Ambruso DR, Rugg N, Worsham DN, Pinkard S, Graminske S, Buck J, Goldberg J, Bill J. Spectra Optia granulocyte apheresis collections result in higher collection efficiency of viable, functional neutrophils in a randomized, crossover, multicenter trial. Transfusion. 2015 55: 748-755.
• Jaeyul Kwon, Ph.D.; Aibing Wang, Ph.D.; Devin J Burke;Howard E Boudreau, Ph.D.; Kristen J Lekstrom, M.S.; AgnieszkaKorzeniowska, M.S.; Ryuichi Sugamata; Yong-Soo Kim, Ph.D.; Liang Li,Ph.D.; Ilker Ersoy, Ph.D.; Stefan Jaeger, Ph.D.; Kannappan Palaniappan,Ph.D.; Daniel R Ambruso, MD; Sharon H Jackson, MD; Thomas L. Leto, Ph.D. Peroxiredoxin 6 (Prdx6) supports NADPH oxidase1 (Nox1)-based reactive oxygen species generation and cell migration. In revision, Free Radical Biology and Medicine.
• Kwon J, Wang A, Burke DJ, Boudreau HE, Lekstrom KJ, Korzeniowska A, Sugamata R, Kim YS, Yi L, Ersoy I, Jaeger S, Palaniappan K, Ambruso DR, Jackson SH, Leto TL. Peroxiredoxin 6 (Prdx6) supports NADPH oxidase1 (Nox1)-based superoxide generation and cell migration. Free Radic Biol Med. 2016 Jul;96:99-115. PubMed PMID: 27094494 « Go Back
• Daniel R. Ambruso (MD)1,2, Michael Ellison (PhD)1, Natalie Briones (MS)1, Angelina Baroffio (MS)1. In Vivo Effects of Interferon-gamma 1-b (IFN-?) on Neutrophils. 2016. Blood (Abstracts for American Society for Hematology national meeting, December3-6, 2016.
• Michael A Ellison1,*, Christy M Gearheart1,*, Christopher C Porter1 and Daniel R Ambruso1,2,3,4 . IFN-gamma Alters the Expression of Diverse Immunity Related Genes in a Cell Culture Model of Maturing Bone Marrow Neutrophils. 2016. Blood (Abstracts for American Society for Hematology national meeting, December 3-6, 2016
• Christopher McKinney, MD1,2, Michael Ellison, PhD2, Natalie Briones, MS2, Angelina Baroffio, MS2, Julie A Reisz, PhD3, Angelo D’alessandro, PhD3, Daniel R Ambruso, MD1,2. Metabolic Abnormalities in G6PC3 Deficient Human Neutrophils Result in Severe Functional Defects. 2016 Blood (Abstracts for the American Society of Hematology national meeting, December 3-6, 2016.
• Ambruso DR, Hauk, P: Primary Immunodeficiency: Chronic Granulomatous Disease and Other Immunodeficiency Disorders. In Kendig and Chernick’s Disorders of the Respiratory Tract in Children. RW Wilmott, TF Boat, A Bush, V Chernick, R Deterding, F Ratjen (eds), WB Saunders Co., 2017.
• Ellison Michael A., Gearheart Christy M, Porter Christopher C., Ambruso Daniel R.. IFN-gamma Alters the Expression of Diverse Immunity Related Genes in a Cell Culture Model Designed to Represent Maturing Neutrophils PLOS ONE https://doi.org/10.1371/journalpone.0185956. October 5, 2017
• Ambruso Daniel R. and Hauk Pia. Primary Imminodeficiency and Other Diseases of Immune Dysregulation. in Kendig and Cherniks Disorders of the Respiratory Track in Children. Wilmott, Boat, Bush, Chernick, Deterding and Ratjen, eds..Elsevier, Philadelphia, PA
• Ambruso Daniel R, Nuss Rachelle, Wong Michael. Hematologic Disorders in Current Pediatric Diagnosis and Treatment, William W. Hay, Jr, Myron J. Levin, Robin R. Deterding, Mark J. Abzug, MD, eds.. Lange Medical Books/McGraw-Hill, Chicargo
• Brown Megan C., Capocelli Kelley, Hanna Melisha , Daniel Ambruso. Development of immune complex mediated glomerulonephritis suggestive of lupus nephritis in pediatric patient with chronic granulomatous disease. (in preparation)
• Ambruso, Daniel R., Nuss, Rachelle, Wong, Michael. Hematolo-Hillic Disorders. Current Pediatric Diagnosis and Treatment. William W. Hay, Jr., Myron J. Levin, Robin R. Deterding and Mark J. Abzug, eds. Lange Medical Books/ McGraw-Hill Education, Chicago, IL.
• Ambruso, Daniel R. and Hauk, Pia J. Primary Immunodeficiency and Other Diseases with Immune Dysregulation. in Disorders of the Respiratory Tract in Children. Robert W. Wilmott, Robin Deterding, Albert Li, Felix Ratjen, Peter Sly, Heather J. Zar, and Andrew Bush eds. Elsevier, Philadelphia, PA.
• Michael Ellison, Natalie Briones, Angelina Baroffio, John Murphy, Katherine Gowan, Bridget Sanford, Kenneth L. Jones, and Daniel R. Ambruso. Interferon-Gamma Induced Changes in Gene Expression Dramatically Alter Phenotype. Submitted
• Christopher McKinney, Michael Ellison, Natalie Briones, Angelina Baroffio, John Murphy, Julie Reisz, Angelo D'Alessandro, and Daniel R. Ambruso. Metabolic Abnormalities in G6PC3-Deficient Human Neutrophils Result in Severe Functional Defects (in Preparation)
• Christopher McKinney, MD, Michael Ellison, PhD, Natalie Briones, MS, Angelina Baroffio, MS, John Murphy, BS, Julie A Reisz, PhD, Angelo D’alessandro, PhD, Daniel R Ambruso, MD. Metabolic Abnormalities in G6PC3-Deficient Human Neutrophils Result in Severe Functional Defects. (in preparation)

Professional Memberships

• American Pediatric Society (APS), Member
• Denver and Colorado Medical Societies , Member
• American Society of Hematology, Member
• AABB, formerly American Association of Blood Banks , Member
• Society For Pediatric Research (SPR), Member
• Western Society for Pediatric Research (WSPR), Member
• Society for Pediatric Hematology Oncology, Member

Practice Locations

Children's Hospital Colorado Anschutz Medical Campus
13123 East 16th Ave
Aurora, CO 80045
720-777-1234

UCHealth Hematology Clinic - Anschutz Medical Campus
1665 Aurora Ct
2nd Floor, Anschutz Cancer Pavilion
Aurora, CO 80045
720-848-0300

Hospital Affiliation

• Children's Hospital Colorado
• University of Colorado Hospital

Specialty Information

Specialties

• Pediatrics, Board Certification (1978)
• Pediatric Hematology / Oncology, Board Certification (1978, 2010)

Conditions & Treatments

• Blood / Lymphatic System - Blood Disorders
• Cancers
• Blood / Lymphatic System - Bleeding Disorders
• Blood / Lymphatic System