Andrew Bradford, PhD

Publications

• Kucherov A, Polotsky, AJ, Menke, M, Isaac, B, McAvey, B, Buyuk, E, Bradford, AP, Hickmon, C Babbs, B, Berga, S, Loucks, T and Santoro, N. Aromatase inhibition causes increased amplitude, but not frequency, of hypothalamic-pituitary output in normal women. Fertil. Steril 2011. In Press
• Regulation of Protein Kinase C? Expression and Sensitivity to Chemotherapy in Endometrial Cancers. Alicia M Thorne and Andrew P. Bradford, Endocrine Society Annual Meeting 2010. Poster presentation.
• DNA profiling analysis of endometrial and ovarian cell lines reveals misidentification, redundancy and contamination. Korch C, Spillman MA, Jackson TA, Jacobsen BM, Murphy SK, Lessey BA, Jordan VC, Bradford AP. Gynecol Oncol. 2012 Oct;127(1):241-8
• Expression of Six1 in luminal breast cancers predicts poor prognosis and promotes increases in tumor initiating cells by activation of extracellular signal-regulated kinase and transforming growth factor-beta signaling pathways. Iwanaga R, Wang CA, Micalizzi DS, Harrell JC, Jedlicka P, Sartorius CA, Kabos P, Farabaugh SM, Bradford AP, Ford HL. Breast Cancer Res. 2012 Jul 5;14(4):R100
• Assessment of luteal function in the vervet monkey as a means to develop a model for obesity-related reproductive phenotype. Kundu MC, May MC, Chosich J, Bradford AP, Lasley B, Gee N, Santoro N, Appt SE, Polotsky AJ. Syst Biol Reprod Med. 2013 Jan 2
• Dai D, Bradford AP, Prossnitz ER. Endometrial cancer: molecular and cellular basis of tumor development, novel biomarkers and therapeutic agents, and innovative research approaches. Obstet Gynecol Int. 2014;2014:710405. PubMed PMID: 24550988
• Characterization of the uterine leiomyoma microRNAome by deep sequencing. Georgieva B, Milev I, Minkov I, Dimitrova I, Bradford AP, Baev V. Genomics. 2012 May;99(5):275-81
• Ross LA, Polotsky AJ, Kucherov A, Bradford AP, Lesh J, Chosich J, Gee N, Santoro N. Profound reduction of ovarian estrogen by aromatase inhibition in obese women. Obesity (Silver Spring). 2014 Jan 31. [Epub ahead of print] PubMed PMID: 24482405
• Wooding KM, Barkley RM, Hankin JA, Johnson CA, Bradford AP, Santoro N, Murphy RC. Mechanism of formation of the major estradiol product ions following collisional activation of the molecular anion in a tandem quadrupole mass spectrometer. J Am Soc Mass Spectrom. 2013 Oct;24(10):1451-5. PubMed PMID: 23955001
• Thorne AM, Jackson TA, Willis VC, Bradford AP. Protein Kinase C a Modulates Estrogen-Receptor-Dependent Transcription and Proliferation in Endometrial Cancer Cells. Obstet Gynecol Int. 2013;2013:537479. PubMed PMID: 23843797
• Kundu MC, May MC, Chosich J, Bradford AP, Lasley B, Gee N, Santoro N, Appt SE, Polotsky AJ. Assessment of luteal function in the vervet monkey as a means to develop a model for obesity-related reproductive phenotype. Syst Biol Reprod Med. 2013 Apr;59(2):74-81. PubMed PMID: 23278149
• Merhi Z, Polotsky AJ, Bradford AP, Buyuk E, Chosich J, Phang T, Jindal S, Santoro N. Adiposity Alters Genes Important in Inflammation and Cell Cycle Division in Human Cumulus Granulosa Cell. Reprod Sci. 2015 Feb 11. [Epub ahead of print] PubMed PMID: 25676576
• Wooding KM, Hankin JA, Johnson CA, Chosich JD, Baek SW, Bradford AP, Murphy RC, Santoro N. Measurement of estradiol, estrone, and testosterone in postmenopausal human serum by isotope dilution liquid chromatography tandem mass spectrometry without derivatization. Steroids. 2015 Apr;96:89-94. PubMed PMID: 25617740
• Kurayoshi K, Ozono E, Iwanaga R, Bradford AP, Komori H, Ohtani K. Cancer cell specific cytotoxic gene expression mediated by ARF tumor suppressor promoter constructs. Biochem Biophys Res Commun. 2014 Jul 18;450(1):240-6. PubMed PMID: 24893334
• Al-Safi ZA, Liu H, Carlson NE, Chosich J, Harris M, Bradford AP, Robledo C, Eckel RH, Polotsky AJ. Omega-3 Fatty Acid Supplementation Lowers Serum FSH in Normal Weight But Not Obese Women. J Clin Endocrinol Metab. 2016 Jan;101(1):324-33. PubMed PMID: 26523525
• Al-Safi ZA, Liu H, Carlson NE, Chosich J, Lesh J, Robledo C, Bradford AP, Gee NA, Phang T, Santoro N, Kohrt W, Polotsky AJ. Estradiol Priming Improves Gonadotrope Sensitivity and Pro-Inflammatory Cytokines in Obese Women. J Clin Endocrinol Metab. 2015 Nov;100(11):4372-81. PubMed PMID: 26425884
• Noshchenko A, Hoffecker L, Lindley EM, Burger EL, Cain CM, Patel VV, Bradford AP. Predictors of spine deformity progression in adolescent idiopathic scoliosis: A systematic review with meta-analysis. World J Orthop. 2015 Aug 18;6(7):537-58. PubMed PMID: 26301183
• Roth LW, Bradshaw-Pierce EL, Allshouse AA, Lesh J, Chosich J, Bradford AP, Polotsky AJ, Santoro N. Evidence of GnRH antagonist escape in obese women. J Clin Endocrinol Metab. 2014 May;99(5):E871-5. PubMed PMID: 24650013
• Bradford AP, Jones K, Kechris K, Chosich J, Montague M, Warren WC, May MC, Al-Safi Z, Kuokkanen S, Appt SE, Polotsky AJ. Joint MiRNA/mRNA expression profiling reveals changes consistent with development of dysfunctional corpus luteum after weight gain. PLoS One. 2015;10(8):e0135163. PubMed PMID: 26258540
• Kitamura H, Ozono E, Iwanaga R, Bradford AP, Okuno J, Shimizu E, Kurayoshi K, Kugawa K, Toh H, Ohtani K. Identification of novel target genes specifically activated by deregulated E2F in human normal fibroblasts. Genes Cells. 2015 Sep;20(9):739-57. PubMed PMID: 26201719
• Merhi Z, Polotsky AJ, Bradford AP, Buyuk E, Chosich J, Phang T, Jindal S, Santoro N. Adiposity Alters Genes Important in Inflammation and Cell Cycle Division in Human Cumulus Granulosa Cell. Reprod Sci. 2015 Oct;22(10):1220-8. PubMed PMID: 25676576
• Wooding KM, Hankin JA, Johnson CA, Chosich JD, Baek SW, Bradford AP, Murphy RC, Santoro N. Measurement of estradiol, estrone, and testosterone in postmenopausal human serum by isotope dilution liquid chromatography tandem mass spectrometry without derivatization. Steroids. 2015 Apr;96:89-94. PubMed PMID: 25617740
• Roth LW, Allshouse AA, Bradshaw-Pierce EL, Lesh J, Chosich J, Kohrt W, Bradford AP, Polotsky AJ, Santoro N. Luteal phase dynamics of follicle-stimulating and luteinizing hormones in obese and normal weight women. Clin Endocrinol (Oxf). 2014 Sep;81(3):418-25. PubMed PMID: 24576183
• Dai D, Bradford AP, Prossnitz ER. Endometrial cancer: molecular and cellular basis of tumor development, novel biomarkers and therapeutic agents, and innovative research approaches. Obstet Gynecol Int. 2014;2014:710405. PubMed PMID: 24550988
• Chosich J, Bradford AP, Allshouse AA, Reusch JE, Santoro N, Schauer IE. Acute recapitulation of the hyperinsulinemia and hyperlipidemia characteristic of metabolic syndrome suppresses gonadotropins. Obesity (Silver Spring). 2017 Feb 3. [Epub ahead of print] PubMed PMID: 28158916
• Kurayoshi K, Shiromoto A, Ozono E, Iwanaga R, Bradford AP, Araki K, Ohtani K. Ectopic expression of the CDK inhibitor p21(Cip1) enhances deregulated E2F activity and increases cancer cell-specific cytotoxic gene expression mediated by the ARF tumor suppressor promoter. Biochem Biophys Res Commun. 2017 Jan 29;483(1):107-114. PubMed PMID: 28042030
• Kurayoshi K, Okuno J, Ozono E, Iwanaga R, Bradford AP, Kugawa K, Araki K, Ohtani K. The phosphatidyl inositol 3 kinase pathway does not suppress activation of the ARF and BIM genes by deregulated E2F1 activity. Biochem Biophys Res Commun. 2017 Jan 22;482(4):784-790. PubMed PMID: 27888102
• Kuokkanen S, Polotsky AJ, Chosich J, Bradford AP, Jasinska A, Phang T, Santoro N, Appt SE. Corpus luteum as a novel target of weight changes that contribute to impaired female reproductive physiology and function. Syst Biol Reprod Med. 2016 Aug;62(4):227-42. PubMed PMID: 27187064
• Ross LA, Polotsky AJ, Kucherov A, Bradford AP, Lesh J, Chosich J, Gee N, Santoro N. Profound reduction of ovarian estrogen by aromatase inhibition in obese women. Obesity (Silver Spring). 2014 Jun;22(6):1464-9. PubMed PMID: 24482405
• Al-Safi ZA, Liu H, Carlson NE, Chosich J, Harris M, Bradford AP, Robledo C, Eckel RH, Polotsky AJ. Omega-3 Fatty Acid Supplementation Lowers Serum FSH in Normal Weight But Not Obese Women. J Clin Endocrinol Metab. 2016 Jan;101(1):324-33. PubMed PMID: 26523525
• Ozono E, Bradford AP, Iwanaga R, Kurayoshi K, Araki K, Ohtani K. Deregulated E2F activity: Novel targets and therapeutic potential in cancer treatment. KEIjournals Medical Research Archives. 2016 Nov 1 (3), 286. doi:10.18103/ibr.v0i3.286
• Al-Safi ZA, Polotsky A, Chosich J, Roth L, Allshouse AA, Bradford AP, Santoro N. Evidence for disruption of normal circadian cortisol rhythm in women with obesity. Gynecol Endocrinol. 2017 Oct 25;:1-5. [Epub ahead of print] PubMed PMID: 29068243
• Chosich J, Bradford AP, Allshouse AA, Reusch JE, Santoro N, Schauer IE. Acute recapitulation of the hyperinsulinemia and hyperlipidemia characteristic of metabolic syndrome suppresses gonadotropins. Obesity (Silver Spring). 2017 Mar;25(3):553-560. PubMed PMID: 28158916
• Kurayoshi K, Shiromoto A, Ozono E, Iwanaga R, Bradford AP, Araki K, Ohtani K. Ectopic expression of the CDK inhibitor p21(Cip1) enhances deregulated E2F activity and increases cancer cell-specific cytotoxic gene expression mediated by the ARF tumor suppressor promoter. Biochem Biophys Res Commun. 2017 Jan 29;483(1):107-114. PubMed PMID: 28042030
• Komori H, Goto Y, Kurayoshi K, Ozono E, Iwanaga R, Bradford AP, Araki K, Ohtani K. Differential requirement for dimerization partner DP between E2F-dependent activation of tumor suppressor and growth-related genes. Sci Rep. 2018 May 31;8(1):8438. PubMed PMID: 29855511
• Al-Safi ZA, Polotsky A, Chosich J, Roth L, Allshouse AA, Bradford AP, Santoro N. Evidence for disruption of normal circadian cortisol rhythm in women with obesity. Gynecol Endocrinol. 2018 Apr;34(4):336-340. PubMed PMID: 29068243
• Kurayoshi K, Okuno J, Ozono E, Iwanaga R, Bradford AP, Kugawa K, Araki K, Ohtani K. The phosphatidyl inositol 3 kinase pathway does not suppress activation of the ARF and BIM genes by deregulated E2F1 activity. Biochem Biophys Res Commun. 2017 Jan 22;482(4):784-790. PubMed PMID: 27888102
• Ozono E, Bradford AP, Iwanaga R, Kurayoshi K, Araki K, Ohtani K. Deregulated E2F activity: Novel targets and therapeutic potential in cancer treatment. KEIjournals Medical Research Archives. 2016 Nov 1 (3), 286. doi:10.18103/ibr.v0i3.286
• Corral J, Post M & Bradford A (2017): Just-in-Time Faculty Development for Pathology Small Groups, Medical Science Educator, DOI: 10.1007/s40670-017-0516-z
• Adams CL, Dimitrova I, Post MD, Gibson L, Spillman MA, Behbakht K, Bradford AP. Identification of a novel diagnostic gene expression signature to discriminate uterine leiomyoma from leiomyosarcoma. Exp Mol Pathol. 2019 Oct;110:104284. PubMed PMID: 31301306
• Bauer JL, Kuhn K, Bradford AP, Al-Safi ZA, Harris MA, Eckel RH, Robledo CY, Malkhasyan A, Johnson J, Gee NR, Polotsky AJ. Reduction in FSH Throughout the Menstrual Cycle After Omega-3 Fatty Acid Supplementation in Young Normal Weight but not Obese Women. Reprod Sci. 2019 Aug;26(8):1025-1033. PubMed PMID: 30773100
• Komori H, Goto Y, Kurayoshi K, Ozono E, Iwanaga R, Bradford AP, Araki K, Ohtani K. Differential requirement for dimerization partner DP between E2F-dependent activation of tumor suppressor and growth-related genes. Sci Rep. 2018 May 31;8(1):8438. PubMed PMID: 29855511
• Al-Safi ZA, Polotsky A, Chosich J, Roth L, Allshouse AA, Bradford AP, Santoro N. Evidence for disruption of normal circadian cortisol rhythm in women with obesity. Gynecol Endocrinol. 2018 Apr;34(4):336-340. PubMed PMID: 29068243
• Jones K, Ryan S, Carlson NE, Chosich J, Bradford AP, Santoro N, Polotsky AJ. Aromatase Inhibition Ameliorates Decreased LH Output Found in Obese Women. Reprod Sci. 2020 Apr;27(4):1018-1023. PubMed PMID: 32046430
• Adams CL, Dimitrova I, Post MD, Gibson L, Spillman MA, Behbakht K, Bradford AP. Identification of a novel diagnostic gene expression signature to discriminate uterine leiomyoma from leiomyosarcoma. Exp Mol Pathol. 2019 Oct;110:104284. PubMed PMID: 31301306
• Bauer JL, Kuhn K, Bradford AP, Al-Safi ZA, Harris MA, Eckel RH, Robledo CY, Malkhasyan A, Johnson J, Gee NR, Polotsky AJ. Reduction in FSH Throughout the Menstrual Cycle After Omega-3 Fatty Acid Supplementation in Young Normal Weight but not Obese Women. Reprod Sci. 2019 Aug;26(8):1025-1033. PubMed PMID: 30773100
• Moroney MR, Woodruff E, Qamar L, Bradford AP, Wolsky R, Bitler BG, Corr BR. Inhibiting Wnt/beta-catenin in CTNNB1-mutated endometrial cancer. Mol Carcinog. 2021 Aug;60(8):511-523. PubMed PMID: 34038589
• Santoro N, Schauer IE, Kuhn K, Fought AJ, Babcock-Gilbert S, Bradford AP. Gonadotropin response to insulin and lipid infusion reproduces the reprometabolic syndrome of obesity in eumenorrheic lean women: a randomized crossover trial. Fertil Steril. 2021 Aug;116(2):566-574. PubMed PMID: 33838870
• Tannous A, Bradford AP, Kuhn K, Fought A, Schauer I, Santoro N. A randomised trial examining inflammatory signaling in acutely induced hyperinsulinemia and hyperlipidemia in normal weight women-the reprometabolic syndrome. PLoS One. 2021;16(3):e0247638. PubMed PMID: 33764994
• Jones K, Ryan S, Carlson NE, Chosich J, Bradford AP, Santoro N, Polotsky AJ. Aromatase Inhibition Ameliorates Decreased LH Output Found in Obese Women. Reprod Sci. 2020 Apr;27(4):1018-1023. PubMed PMID: 32046430
• Santoro NF, Kohn SA, Fought AJ, Kuhn K, Jones K, Bradford AP, Schauer I (2021). Heparin effects on serum gonadotropins. Journal Endocrine Society, in press. https://doi.org/10.1210/jendso/bvab178
• McDonald R, Kuhn K, Nguyen TB, Tannous A, Schauer I, Santoro N, Bradford AP. A randomized clinical trial demonstrating cell type specific effects of hyperlipidemia and hyperinsulinemia on pituitary function. PLoS One. 2022;17(5):e0268323. PubMed PMID: 35544473
• Kohn SA, Fought AJ, Kuhn K, Jones Slogett K, Bradford AP, Santoro N, Schauer I. Heparin Effects on Serum Gonadotropins. J Endocr Soc. 2022 Jan 1;6(1):bvab178. PubMed PMID: 35024539
• Moroney MR, Woodruff E, Qamar L, Bradford AP, Wolsky R, Bitler BG, Corr BR. Inhibiting Wnt/beta-catenin in CTNNB1-mutated endometrial cancer. Mol Carcinog. 2021 Aug;60(8):511-523. PubMed PMID: 34038589
• Santoro N, Schauer IE, Kuhn K, Fought AJ, Babcock-Gilbert S, Bradford AP. Gonadotropin response to insulin and lipid infusion reproduces the reprometabolic syndrome of obesity in eumenorrheic lean women: a randomized crossover trial. Fertil Steril. 2021 Aug;116(2):566-574. PubMed PMID: 33838870
• Tannous A, Bradford AP, Kuhn K, Fought A, Schauer I, Santoro N. A randomised trial examining inflammatory signaling in acutely induced hyperinsulinemia and hyperlipidemia in normal weight women-the reprometabolic syndrome. PLoS One. 2021;16(3):e0247638. PubMed PMID: 33764994
• Luu TH, Kuhn K, Bradford AP, Wempe MF, Wittenburg L, Johnson RL, Carlson NE, Kumar TR, Polotsky AJ. Effects of pulsatile intravenous follicle-stimulating hormone treatment on ovarian function in women with obesity. Fertil Steril. 2023 Oct;120(4):890-898. PubMed PMID: 37276947
• Nakajima R, Deguchi R, Komori H, Zhao L, Zhou Y, Shirasawa M, Angelina A, Goto Y, Tohjo F, Nakahashi K, Nakata K, Iwanaga R, Bradford AP, Araki K, Warita T, Ohtani K. The TFDP1 gene coding for DP1, the heterodimeric partner of the transcription factor E2F, is a target of deregulated E2F. Biochem Biophys Res Commun. 2023 Jun 30;663:154-162. PubMed PMID: 37141667
• Nakajima R, Zhao L, Zhou Y, Shirasawa M, Uchida A, Murakawa H, Fikriyanti M, Iwanaga R, Bradford AP, Araki K, Warita T, Ohtani K. Deregulated E2F Activity as a Cancer-Cell Specific Therapeutic Tool. Genes (Basel). 2023 Feb 2;14(2). PubMed PMID: 36833320
• Santoro N, Kuhn K, Pretzel S, Schauer I E, Fought A, D’Alessandro A, Stephenson D, Bradford AP, A High-Fat Eucaloric Diet Induces Reprometabolic Syndrome of Obesity in Normal Weight Women, 2023 PNAS Nexus, in press
• Zhou, Y.; Nakajima, R.; Shirasawa, M.; Fikriyanti, M.; Zhao, L.; Iwanaga, R.; Bradford, A.P.; Kurayoshi, K.; Araki, K.; Ohtani, K. Expanding Roles of the E2F-RB-p53 Pathway in Tumor Suppression. Biology 2023, 12, 1511. https:// doi.org/10.3390/biology12121511
• Zhao L, Nakajima R, Zhou Y, Shirasawa M, Fikriyanti M, Kamiya Y, Toh H, Komori H, Iwanaga R, Bradford AP, Nishitani H, Kurayoshi K, Araki K, Ohtani K. The N-Terminal Region of the Transcription Factor E2F1 Contains a Novel Transactivation Domain and Recruits General Transcription Factor GTF2H2. Biomolecules. 2024 Oct 25;14(11). PubMed PMID: 39595534
• Bradford AP, Nguyen T, Kuhn K, Santoro N. Reply of the authors: evaluating the hormonal impact of high-fat diets: gaps in gonadotropin and adrenocorticotropic hormone responses and the need for detailed fat composition analysis. Fertil Steril. 2024 Oct 15. [Epub ahead of print] PubMed PMID: 39419318
• Shirasawa M, Nakajima R, Zhou Y, Zhao L, Fikriyanti M, Iwanaga R, Bradford AP, Kurayoshi K, Araki K, Ohtani K. Activation of the CDK7 Gene, Coding for the Catalytic Subunit of the Cyclin-Dependent Kinase (CDK)-Activating Kinase (CAK) and General Transcription Factor II H, by the Trans-Activator Protein Tax of Human T-Cell Leukemia Virus Type-1. Genes (Basel). 2024 Aug 15;15(8). PubMed PMID: 39202439
• Hayati RF, Nakajima R, Zhou Y, Shirasawa M, Zhao L, Fikriyanti M, Iwanaga R, Bradford AP, Kurayoshi K, Araki K, Ohtani K. Trans-Activation of the Coactivator-Associated Arginine Methyltransferase 1 (Carm1) Gene by the Oncogene Product Tax of Human T-Cell Leukemia Virus Type 1. Genes (Basel). 2024 May 27;15(6). PubMed PMID: 38927636
• Nguyen T, Kuhn K, Fought A, Bolt M, Bradford AP, Santoro N. Effects of a eucaloric high-fat diet on anterior pituitary hormones and adipocytokines in women with normal weight. Fertil Steril. 2024 Aug;122(2):379-381. PubMed PMID: 38838803
• Nguyen T, Kuhn K, Bolt M, Duffy K, Bradford AP, Santoro N. Analysis of Inflammatory Markers in Response to Induction of Reprometabolic Syndrome by a Eucaloric High Fat Diet in Normal Weight Women. Reprod Sci. 2024 Sep;31(9):2820-2828. PubMed PMID: 38710978
• Santoro N, Hendricks N, Gibbs L, Kuhn K, Bradford AP, Mitchell-Leef D. Acceptability and Feasibility of Initiating a Low Fat Eating Plan in Reproductive Aged Women with Obesity. Reprod Sci. 2024 Aug;31(8):2228-2233. PubMed PMID: 38622475
• Caldwell AE, Gorczyca AM, Bradford AP, Nicklas JM, Montgomery RN, Smyth H, Pretzel S, Nguyen T, DeSanto K, Ernstrom C, Santoro N. Effectiveness of preconception weight loss interventions on fertility in women: a systematic review and meta-analysis. Fertil Steril. 2024 Aug;122(2):326-340. PubMed PMID: 38408693
• Santoro N, Kuhn K, Pretzel S, Schauer IE, Fought A, D'Alessandro A, Stephenson D, Bradford AP. A high-fat eucaloric diet induces reprometabolic syndrome of obesity in normal weight women. PNAS Nexus. 2024 Jan;3(1):pgad440. PubMed PMID: 38178979
• Zhou Y, Nakajima R, Shirasawa M, Fikriyanti M, Zhao L, Iwanaga R, Bradford AP, Kurayoshi K, Araki K, Ohtani K. Expanding Roles of the E2F-RB-p53 Pathway in Tumor Suppression. Biology (Basel). 2023 Dec 11;12(12). PubMed PMID: 38132337