Anna Malykhina, PhD

Research Interests

Mechanisms of chronic pelvic pain, pelvic organ cross-sensitization, dysfunctional voiding, detrusor over- and underactivity, interplay between urologic and nervous systems, and neurogenic bladder dysfunction

Publications

• Malykhina AP, Grundy L. Special Section on Therapeutic Approaches to Treat Disorders of the Urinary and Gastrointestinal Tracts-Editorial. J Pharmacol Exp Ther. 2024 Jul 18;390(2):186-187. PubMed PMID: 39025654
• Iguchi N, Teimouri A, Wilcox DT, Malykhina AP, Cost NG. Lower urinary dysfunction as a long-term effect of childhood vincristine treatment, with potential influences by sex and dose. Sci Rep. 2024 Jul 1;14(1):15049. PubMed PMID: 38951167
• Xie AX, Iguchi N, Malykhina AP. Long-term follow-up of TREK-1 KO mice reveals the development of bladder hypertrophy and impaired bladder smooth muscle contractility with age. Am J Physiol Renal Physiol. 2024 Jun 1;326(6):F957-F970. PubMed PMID: 38601986
• Ashraf S, Clarkson T, Malykhina AP. Therapeutic Approaches for Urologic Chronic Pelvic Pain Syndrome; Management: Research Advances, Experimental Targets, and Future Directions. J Pharmacol Exp Ther. 2024 Jul 18;390(2):222-232. PubMed PMID: 38565309
• Feng B, Malykhina AP. Editorial: Emerging trends in abdominal and pelvic pain. Front Pain Res (Lausanne). 2023;4:1254682. PubMed PMID: 37711988
• Iguchi N, Dönmez MI, Malykhina AP, Wilcox DT. Anti-fibrotic effect of tocotrienols for bladder dysfunction due to partial bladder outlet obstruction. Investig Clin Urol. 2023 Mar;64(2):189-196. PubMed PMID: 36882179
• Feng B, Malykhina AP. Editorial: Emerging trends in abdominal and pelvic pain. Front Pain Res (Lausanne). 2023;4:1254682. PubMed PMID: 37711988
• Iguchi N, Dönmez MI, Malykhina AP, Wilcox DT. Anti-fibrotic effect of tocotrienols for bladder dysfunction due to partial bladder outlet obstruction. Investig Clin Urol. 2023 Mar;64(2):189-196. PubMed PMID: 36882179
• Clarkson TC, Iguchi N, Xie AX, Malykhina AP. Differential transcriptomic changes in the central nervous system and urinary bladders of mice infected with a coronavirus. PLoS One. 2022;17(12):e0278918. PubMed PMID: 36490282
• Iguchi N, Hecht SL, Gao D, Wilcox DT, Malykhina AP, Cost NG. Sexual dimorphic impacts of systemic vincristine on lower urinary tract function. Sci Rep. 2022 Mar 24;12(1):5113. PubMed PMID: 35332157
• Xie AX, Iguchi N, Clarkson TC, Malykhina AP. Pharmacogenetic inhibition of lumbosacral sensory neurons alleviates visceral hypersensitivity in a mouse model of chronic pelvic pain. PLoS One. 2022;17(1):e0262769. PubMed PMID: 35077502
• Iguchi N, Hecht SL, Gao D, Wilcox DT, Malykhina AP, Cost NG. Sexual dimorphic impacts of systemic vincristine on lower urinary tract function. Sci Rep. 2022 Mar 24;12(1):5113. PubMed PMID: 35332157
• Xie AX, Iguchi N, Clarkson TC, Malykhina AP. Pharmacogenetic inhibition of lumbosacral sensory neurons alleviates visceral hypersensitivity in a mouse model of chronic pelvic pain. PLoS One. 2022;17(1):e0262769. PubMed PMID: 35077502
• Hecht SL, Quach A, Gao D, Brazell A, Beltran G, Holbrook S, Gore L, Iguchi N, Malykhina A, Wilcox D, Cost NG. A prospective survey study of lower urinary tract dysfunction in childhood cancer survivors after vincristine and/or doxorubicin chemotherapy. Pediatr Blood Cancer. 2021 Oct;68(10):e29226. PubMed PMID: 34245214
• Soriano A, Allen A, Malykhina AP, Andy U, Harvie H, Arya L. Relationship of Pain Catastrophizing With Urinary Biomarkers in Women With Bladder Pain Syndrome. Female Pelvic Med Reconstr Surg. 2021 Dec 1;27(12):746-752. PubMed PMID: 33787562
• Lloyd GL, Wiesen B, Atwell M, Malykhina A. Marijuana, Alcohol, and ED: Correlations with LUTS/BPH. Curr Urol Rep. 2021 Feb 8;22(4):21. PubMed PMID: 33554319
• Iguchi N, Carrasco A Jr, Xie AX, Pineda RH, Malykhina AP, Wilcox DT. Functional constipation induces bladder overactivity associated with upregulations of Htr2 and Trpv2 pathways. Sci Rep. 2021 Jan 13;11(1):1149. PubMed PMID: 33441874
• Smith AL, Weissbart SJ, Hartigan SM, Bilello M, Newman DK, Wein AJ, Malykhina AP, Erus G, Fan Y. Association between urinary symptom severity and white matter plaque distribution in women with multiple sclerosis. Neurourol Urodyn. 2020 Jan;39(1):339-346. PubMed PMID: 31691357
• Xie AX, Madayag A, Minton SK, McCarthy KD, Malykhina AP. Sensory satellite glial Gq-GPCR activation alleviates inflammatory pain via peripheral adenosine 1 receptor activation. Sci Rep. 2020 Aug 25;10(1):14181. PubMed PMID: 32843670
• Soriano A, Andy U, Hassani D, Whitmore K, Harvie H, Malykhina AP, Arya L. Relationship of Bladder Pain With Clinical and Urinary Markers of Neuroinflammation in Women With Urinary Urgency Without Urinary Incontinence. Female Pelvic Med Reconstr Surg. 2020 Oct 1. [Epub ahead of print] PubMed PMID: 33009262
• Smith AL, Weissbart SJ, Hartigan SM, Bilello M, Newman DK, Wein AJ, Malykhina AP, Erus G, Fan Y. Association between urinary symptom severity and white matter plaque distribution in women with multiple sclerosis. Neurourol Urodyn. 2020 Jan;39(1):339-346. PubMed PMID: 31691357
• Lloyd G.L., Wiesen B., Atwell M., Malykhina A., Marijuana, Alcohol, and ED: Correlations with LUTS/BPH". Current Urology Reports (in Press)
• Lee S, Nedumaran B, Hypolite J, Caldwell B, Rudolph MC, Malykhina AP. Differential neurodegenerative phenotypes are associated with heterogeneous voiding dysfunction in a coronavirus-induced model of multiple sclerosis. Sci Rep. 2019 Jul 26;9(1):10869. PubMed PMID: 31350464
• Iguchi N, Dönmez MI, Carrasco A Jr, Wilcox DT, Pineda RH, Malykhina AP, Cost NG. Doxorubicin induces detrusor smooth muscle impairments through myosin dysregulation, leading to a risk of lower urinary tract dysfunction. Am J Physiol Renal Physiol. 2019 Jul 1;317(1):F197-F206. PubMed PMID: 31066574
• Pineda RH, Hypolite J, Lee S, Carrasco A Jr, Iguchi N, Meacham RB, Malykhina AP. Altered detrusor contractility and voiding patterns in mice lacking the mechanosensitive TREK-1 channel. BMC Urol. 2019 May 21;19(1):40. PubMed PMID: 31113422
• Nedumaran B, Pineda RH, Rudra P, Lee S, Malykhina AP. Association of genetic polymorphisms in the pore domains of mechano-gated TREK-1 channel with overactive lower urinary tract symptoms in humans. Neurourol Urodyn. 2019 Jan;38(1):144-150. PubMed PMID: 30350878
• Nedumaran B, Pineda RH, Rudra P, Lee S, Malykhina AP. Association of genetic polymorphisms in the pore domains of mechano-gated TREK-1 channel with overactive lower urinary tract symptoms in humans. Neurourol Urodyn. 2019 Jan;38(1):144-150. PubMed PMID: 30350878
• Xin AX, Pan X.-Q, Meacham R.B., Malykhina A.P. The Expression of Transcription Factors MeCP2 and CREB Is Modulated in Inflammatory Pelvic Pain. Front System Neurosci
• Iguchi N, Malykhina AP, Wilcox DT. Early life voiding dysfunction leads to lower urinary tract dysfunction through alteration of muscarinic and purinergic signaling in the bladder. Am J Physiol Renal Physiol. 2018 Aug 8. [Epub ahead of print] PubMed PMID: 30089034
• Nedumaran B, Rudra P, Gaydos J, Kumar S, Meacham RB, Burnham EL, Malykhina AP. Impact of Regular Cannabis Use on Biomarkers of Lower Urinary Tract Function. Urology. 2017 Nov;109:223.e9-223.e16. PubMed PMID: 28827195
• Iguchi N, Dönmez MI, Malykhina AP, Carrasco A Jr, Wilcox DT. Preventative effects of a HIF inhibitor, 17-DMAG, on partial bladder outlet obstruction-induced bladder dysfunction. Am J Physiol Renal Physiol. 2017 Nov 1;313(5):F1149-F1160. PubMed PMID: 28768664
• Pineda RH, Nedumaran B, Hypolite J, Pan XQ, Wilson S, Meacham RB, Malykhina AP. Altered expression and modulation of the two-pore-domain (K(2P)) mechanogated potassium channel TREK-1 in overactive human detrusor. Am J Physiol Renal Physiol. 2017 Aug 1;313(2):F535-F546. PubMed PMID: 28539337
• Lee S, Malykhina AP. Neuro-tracing approach to study kidney innervation: a technical note. Kidney Res Clin Pract. 2017 Mar;36(1):86-94. PubMed PMID: 28393001
• Richter HE, Moalli P, Amundsen CL, Malykhina AP, Wallace D, Rogers R, Myers D, Paraiso M, Albo M, Shi H, Nolen T, Meikle S, Word RA. Urinary Biomarkers in Women with Refractory Urgency Urinary Incontinence Randomized to Sacral Neuromodulation versus OnabotulinumtoxinA Compared to Controls. J Urol. 2017 Jun;197(6):1487-1495. PubMed PMID: 28089729
• Pennycuff JF, Schutte SC, Hudson CO, Karp DR, Malykhina AP, Northington GM. Urinary neurotrophic peptides in postmenopausal women with and without overactive bladder. Neurourol Urodyn. 2017 Mar;36(3):740-744. PubMed PMID: 27062604
• Malykhina AP, Brodie KE, Wilcox DT. Genitourinary and gastrointestinal co-morbidities in children: The role of neural circuits in regulation of visceral function. J Pediatr Urol. 2017 Apr;13(2):177-182. PubMed PMID: 28392009
• Malykhina AP. How the brain controls urination. Elife. 2017 Dec 4;6. PubMed PMID: 29199949
• Lee S, Carrasco A Jr, Meacham RB, Malykhina AP. Transurethral Instillation Procedure in Adult Male Mouse. J Vis Exp. 2017 Nov 2;(129). PubMed PMID: 29155777
• Iguchi N., Malykhina A.P., Wilcox D.T. (2016) Inhibition of hypoxia-inducible factors reduces bladder hypertrophy and improves bladder function in murine model of partial bladder outlet obstruction. J Urol, Feb 26. pi: S0022-5347(15) 04523-1. PMID: 26926557.
• Weissbart S.J., Pechersky D., Malykhina A., Bavaria T., Parillo L., Arya L.A., Bilello M., Wein A.J., Smith A.L. (2016) The impact of pontine disease on lower urinary tract symptoms in patients with multiple sclerosis. Neurourology Urodynamics, Jan 6. doi: 10.1002/nau.22953. PMID: 26741487.
• Smith AL, Hantsoo L, Malykhina AP, File DW, Valentino R, Wein AJ, Sammel MD, Epperson CN. (2016) Basal and stress-activated hypothalamic pituitary adrenal axis function in postmenopausal women with overactive bladder. Int Urogynecol J., 27(9):1383-91. PMID: 26942596.
• Pennycuff J.F., Schutte S., Hudson C., Karp D., Malykhina A.P., Northington G.M. (2016) Urinary Neurotrophic Peptides in Postmenopausal Women with and without Overactive Bladder. Neu-rourology Urodynamics 2016 Apr 6. [Epub ahead of print]. PMID: 27062604
• Richter H.E., Moalli P., Amundsen C.L., Malykhina A., Rogers R., Myers D., Paraiso M., Albo M., Shi H., Nolen T., Wallace D., Meikle S., Word R.A. A Prospective Evaluation of Urinary Biomarkers in Women Undergoing Randomization to Sacral Neuromodulation versus Onabotulinum Toxin A for Refractory Urgency Urinary Incontinence. ICS Annual meeting, September 13-16, 2016, Tokyo, Ja-pan
• Caldwell B.T., Lee S., Iguchi N, Wilcox D.T., Malykhina A.P. Direct effects of FTY270 therapy on urinary bladder function in mice. SPU meeting, September 9-11, 2016, Dallas, TX
• Hypolite J., Meacham R.B., Malykhina A.P. Spontaneous activity of the human detrusor depends on the ratio between contractile responses to nerve stimulation and anticholinergic effects of protein kinase C activation. AUA annual meeting, May 5-9, 2016, San Diego.
• Richter H.E., Moalli P., Amudsen C., Malykhina A., Rogers R., Myers D., Paraiso M., Albo M., Shi H., Wallace D., Meikle S., Word R.A. The ROSETTA Urinary Marker (RUM) Study. AUA annual meeting, May 5-9, 2016, San Diego, CA
• Lee S., Nedumaran B., Meacham R.B., Malykhina A.P. Recovery from neurologic impairment in murine model of coronavirus-induced demyelination does not directly correlate with improvement of neurogenic bladder dysfunction: long-term follow up study. AUA annual meeting, May 5-9, 2016, San Diego, CA
• Nedumaran B., Pan X.-Q., Meacham R.B., Malykhina A.P Early epigenetic changes in bladder sensory and spinal neurons contribute to visceral hyperalgesia and chronic bladder pain. ASN Meeting, March 19-23, 2016, Denver, CO
• Nedumaran B., Meacham R.B., Malykhina A.P Generation of TRPV1- and Chat-knock-in reporter mice to study urinary bladder pain syndrome. Postdoctoral Research Day, Anschutz Medical Cam-pus, March 11th, 2016, Aurora, CO. Podium Presentation
• Hypolite J.A., Chang S., Wein A.J., Chacko S., Malykhina A.P. Protein kinase C modulates frequency of micturition and non-voiding contractions in the urinary bladder via neurogenic and myo-genic mechanisms. BMC Urol., Apr 21;15(1):34. PMID: 25896919.
• Hypolite J.A., Malykhina A.P. Regulation of urinary bladder function by protein kinase C in physiology and pathophysiology. BMC Urol Nov 4;15(1):110. PMID:26538012.
• Andy U.U., Malykhina A., Harvi H.S., Colavita K, Whitmore K, Arya L.A.. Clinical and Bio-chemical Evidence of Neurogenic Inflammation in Women with Urinary Urgency. J of Minim Invas Gynec 22(3):S21.
• Hypolite J., Pan X.-Q., Wilson S., Meacham R.B., Malykhina A.P. Mechanosensitivity of the human detrusor during bladder filling is regulated by stretch-activated two-pore domain potassium channels. 94th SCS annual meeting, October 28-31, 2015, Scottsdale, AR.
• Hypolite J., Chacko S., Meacham R.B., Malykhina A.P. Protein kinase C modulates nerve-mediated, cholinergic, and spontaneous contractions in isolated human detrusor. 94th SCS annual meeting, October 28-31, 2015, Scottsdale, AR.
• Iguchi N, Malykhina A.P., Wilcox D.T. Blockade of hypoxia-inducible factors improves bladder function in partial bladder outlet obstruction. 94th SCS annual meeting, October 28-31, 2015, Scottsdale, AR
• Hypolite J., Pan X.-Q., Wilson S., Meacham R.B., Malykhina A.P. Impaired expression and function of TREK-1, a stretch-activated two-pore domain potassium channel, underlies detrusor overactivity in humans. AUA annual meeting, May 15-19, 2015, New Orleans
• Iguchi N, Hypolite J., Malykhina A.P., Wilcox D.T. Inhibition of hypoxia-inducible factors reduces bladder hyperthrophy and improves bladder function in murine model of partial bladder outlet obstruction. AUA annual meeting, May 15-19, 2015, New Orleans
• Weissbart S., Malykhina A.P., Bavaria T., Wein A., Smith A., The impact of pontine disease on lower urinary tract symptoms in patients with multiple sclerosis. AUA annual meeting, May 15-19, 2015, New Orleans
• Andy U.U., Malykhina A.P. Clinical and biochemical evidence of neurogenic inflammation in women with urinary urgency, March 22-25, 2015, Annual SGS meeting, Orlando, FL.
• Lamarre NS, Braverman AS, Malykhina AP, Barbe MF, Ruggieri MR Sr. Alterations in nerve-evoked bladder contractions in a coronavirus-induced mouse model of multiple sclerosis. PLoS One. 2014;9(10):e109314. PubMed PMID: 25310403
• McMillan MT, Pan XQ, Smith AL, Newman DK, Weiss SR, Ruggieri MR Sr, Malykhina AP. Coronavirus-induced demyelination of neural pathways triggers neurogenic bladder overactivity in a mouse model of multiple sclerosis. Am J Physiol Renal Physiol. 2014 Sep 1;307(5):F612-22. PubMed PMID: 25007876
• Lei Q, Pan XQ, Chang S, Malkowicz SB, Guzzo TJ, Malykhina AP. Response of the human detrusor to stretch is regulated by TREK-1, a two-pore-domain (K2P) mechano-gated potassium channel. J Physiol. 2014 Jul 15;592(Pt 14):3013-30. PubMed PMID: 24801307
• Pan XQ, Malykhina AP. Estrous cycle dependent fluctuations of regulatory neuropeptides in the lower urinary tract of female rats upon colon-bladder cross-sensitization. PLoS One. 2014;9(5):e94872. PubMed PMID: 24788240
• Malykhina A, Hanno P. How are we going to make progress treating bladder pain syndrome? ICI-RS 2013. Neurourol Urodyn. 2014 Jun;33(5):625-9. PubMed PMID: 24615847
• Moore KH, Malykhina AP. What is the role of covert infection in detrusor overactivity, and other LUTD? ICI-RS 2013. Neurourol Urodyn. 2014 Jun;33(5):606-10. PubMed PMID: 24844724
• Pennycuff J.F., Schutte S. C., Karp D., Hudson C.O., Malykhina A.P., Northington G. Lower urinary tract symptoms and urinary neuropeptides after treatment with vaginal estrogen: a pilot study. AUGS meeting, July 22-26, 2014, Washington DC
• Ruggieri M.R., McMillan M, Pan X-Q , Lamarre N.S., Braverman A.S., Jindal M., O`Donnell C., Newman D.K., Smith A.L., Malykhina A.P. Neurogenic bladder dysfunction in a murine model of multiple sclerosis is caused by coronavirus-induced demyelination of the nervous system. AUA annu-al meeting, May 15-19, 2014, Orlando
• Smith A.L., Bavaria T., Wang M., Newman D.K., Ruggieri M.R. Sr., Malykhina A.P. Lower urinary tract phenotype in men and women with multiple sclerosis. SUFU meeting, Feb 25-Mar 1st, 2014, Miami, FL.
• Newman D.K., Pfohl D., Bavaria T., Smith A.L., Markowitz C., Jacobs D., Pruitt A., Kolson D., Chahin S., Zimmerman V., Miller M., Malykhina A.P. Self-Reported Bladder and Bowel Symptoms in Multiple Sclerosis Patients. CMSC Meeting, Dallas, TX, May 28 -31, 2014.
• Lamarre N.S., Braverman A.S., Malykhina A.P., Ruggieri M.R.Sr., Evidence of neurogenic bladder dysfunction in a mouse model of multiple sclerosis. SUFU meeting, Feb 25-Mar 1st, 2014, Miami, FL.
• Pennycuff J.F., Schutte S. C., Karp D., Hudson C.O., Malykhina A.P., Northington G. The effect of vaginal estrogen on urinary nerve growth factor and symptoms in women with overactive bladder. SUFU meeting, Feb 25-Mar 1st, 2014, Miami, FL.
• Northington G., Pennycuff J.F., Schutte S. C., Karp D., Hudson C.O., Malykhina A.P. Urinary neuro-throphic peptides in postmenopausal age-matched women with and without overactive bladder. SU-FU meeting, Feb 25-Mar 1st, 2014, Miami, FL.
• Iguchi N., Malykhina A.P., Wilcox D.T. Inhibition of hypoxia-inducible factors reduces bladder hypertrophy and improves bladder function in murine model of partial bladder outlet obstruction. J Urol (in Press).
• Malykhina A.P., Brodie K.E., Wilcox D.T. Genitourinary and gastrointestinal co-morbidities in children: role of neural circuits in regulation of visceral function. J Ped Urol
• Lee S., Malykhina A.P. Neuro-tracing approach to study kidney innervation: a technical note. Kidney Res Clin Pract.
• Clarkson T.C., Iguchi N., Xie A.X, Malykhina A.P. Differential transcriptomic changes in the central nervous system and neurogenic bladders of mice infected with a coronavirus. PLOS One