Igor Kogut, PhD

Research Interests

My laboratory is investigating the mechanisms leading to cellular reprogramming and aging, as well as induced Pluripotent Stem Cell (iPSC)-associated cellular rejuvenation. My group’s expertise in producing mRNA and manipulating RNA transfections was crucial for identifying and optimization of a cocktail of factors that can “rejuvenate” human somatic cells, which we can now deliver into cells via non-integrating clinically-relevant RNA molecules. My group is currently exploring the applicability of this somatic cell rejuvenation approach in improving outcomes of skin transplantation. In addition, my laboratory works extensively with genetically corrected patient iPSCs and skin cells derived from these iPSCs as a potential therapeutic tool for the treatment of Epidermolysis Bullosa (EB), a group of severe inherited skin blistering diseases.

Publications

• Inen J, Han CM, Farrel DM, Bilousova G, Kogut I. CIRCLE-Seq for Interrogation of Off-Target Gene Editing. J Vis Exp. 2024 Nov 1;(213). PubMed PMID: 39555799
• Pavlova M, Balaiya V, Flores JC, Ferreyros M, Bush K, Hopkin A, Kogut I, Roop DR, Bilousova G. The Development of an Advanced Model for Multilayer Human Skin Reconstruction In Vivo. Bio Protoc. 2024 Jan 20;14(2):e4919. PubMed PMID: 38268973
• Pavlova M, Balaiya V, Flores JC, Ferreyros M, Bush K, Hopkin A, Kogut I, Roop DR, Bilousova G. The Development of an Advanced Model for Multilayer Human Skin Reconstruction In Vivo. Bio Protoc. 2024 Jan 20;14(2):e4919. PubMed PMID: 38268973
• McGarvey SS, Ferreyros M, Kogut I, Bilousova G. Differentiating Induced Pluripotent Stem Cells Toward Mesenchymal Stem/Stromal Cells. Methods Mol Biol. 2022;2549:153-167. PubMed PMID: 33772462
• Pavlova M, McGarvey SS, Bilousova G, Kogut I. A High-Efficiency Method for the Production of Endothelial Cells from Human Induced Pluripotent Stem Cells. Methods Mol Biol. 2022;2549:169-186. PubMed PMID: 33755906
• Pavlova M, McGarvey SS, Bilousova G, Kogut I. A High-Efficiency Method for the Production of Endothelial Cells from Human Induced Pluripotent Stem Cells. Methods Mol Biol. 2021 (In press)
• Warshauer EM, Brown A, Fuentes I, Shortt J, Gignoux C, Montinaro F, Metspalu M, Youssefian L, Vahidnezhad H, Jacków J, Christiano AM, Uitto J, Fajardo-Ramírez ÓR, Salas-Alanis JC, McGrath JA, Consuegra L, Rivera C, Maier PA, Runfeldt G, Behar DM, Skorecki K, Sprecher E, Palisson F, Norris DA, Bruckner AL, Kogut I, Bilousova G, Roop DR. Ancestral patterns of recessive dystrophic epidermolysis bullosa mutations in Hispanic populations suggest sephardic ancestry. Am J Med Genet A. 2021 Nov;185(11):3390-3400. PubMed PMID: 34435747
• McGarvey SS, Ferreyros M, Kogut I, Bilousova G. Differentiating Induced Pluripotent Stem Cells Toward Mesenchymal Stem/Stromal Cells. Methods Mol Biol. 2021 Mar 27. [Epub ahead of print] PubMed PMID: 33772462
• Pavlova M, McGarvey SS, Bilousova G, Kogut I. A High-Efficiency Method for the Production of Endothelial Cells from Human Induced Pluripotent Stem Cells. Methods Mol Biol. 2021 Mar 24. [Epub ahead of print] PubMed PMID: 33755906
• Butterfield KT, McGrath PS, Han CM, Kogut I, Bilousova G. Generation of an Induced Pluripotent Stem Cell Line with the Constitutive EGFP Reporter. Methods Mol Biol. 2020;2155:11-21. PubMed PMID: 32474864
• McGrath PS, McGarvey SS, Kogut I, Bilousova G. Efficient RNA-Based Reprogramming of Disease-Associated Primary Human Fibroblasts into Induced Pluripotent Stem Cells. Methods Mol Biol. 2020;2117:271-284. PubMed PMID: 31960386
• Diette N, Kogut I, Bilousova G. Generation of a Full-Thickness Human Skin Equivalent on an Immunodeficient Mouse. Methods Mol Biol. 2020;2109:169-183. PubMed PMID: 31119714
• McGrath PS, McGarvey SS, Kogut I, Bilousova G. Efficient RNA-Based Reprogramming of Disease-Associated Primary Human Fibroblasts into Induced Pluripotent Stem Cells. Methods Mol Biol. 2020;2117:271-284. PubMed PMID: 31960386
• Diette N, Kogut I, Bilousova G. Generation of a Full-Thickness Human Skin Equivalent on an Immunodeficient Mouse. Methods Mol Biol. 2019 May 21. [Epub ahead of print] PubMed PMID: 31119714
• Kogut I, McCarthy SM, Pavlova M, Astling DP, Chen X, Jakimenko A, Jones KL, Getahun A, Cambier JC, Pasmooij AMG, Jonkman MF, Roop DR, Bilousova G. High-efficiency RNA-based reprogramming of human primary fibroblasts. Nat Commun. 2018 Feb 21;9(1):745. PubMed PMID: 29467427
• McGrath PS, Diette N, Kogut I, Bilousova G. RNA-based Reprogramming of Human Primary Fibroblasts into Induced Pluripotent Stem Cells. J Vis Exp. 2018 Nov 26;(141). PubMed PMID: 30531717
• Shen C, Liu Y, Yu H, Gulbranson DR, Kogut I, Bilousova G, Zhang C, Stowell MHB, Shen J. The N-peptide-binding mode is critical to Munc18-1 function in synaptic exocytosis. J Biol Chem. 2018 Nov 23;293(47):18309-18317. PubMed PMID: 30275014
• Kogut I, McCarthy SM, Pavlova M, Astling DP, Chen X, Jakimenko A, Jones KL, Getahun A, Cambier JC, Pasmooij AMG, Jonkman MF, Roop DR, Bilousova G. High-efficiency RNA-based reprogramming of human primary fibroblasts. Nat Commun. 2018 Feb 21;9(1):745. PubMed PMID: 29467427
• Kogut I, Bilousova G. Environmental Influences on the Development of Epidermal Progenitors. In Reference Module of Biomedical Sciences, 2018, Elsevier, doi: 10.1016/B978-0-12-801238-3.65469-7
• Kogut I, Roop DR, Bilousova G. Differentiation of Human Induced Pluripotent Stem Cells into a Keratinocyte Lineage. Methods Mol Biol. 2014 Feb 8. [Epub ahead of print] PubMed PMID: 24510784
• Kogut I, Roop DR, Bilousova G. Differentiation of human induced pluripotent stem cells into a keratinocyte lineage. Methods Mol Biol. 2014;1195:1-12. PubMed PMID: 24510784
• Kogut I, Scholz JL, Cancro MP, Cambier JC. B cell maintenance and function in aging. Semin Immunol. 2012 Oct;24(5):342-9. PMID: 22560930
• Getahun A, Smith MJ, Kogut I, van Dyk LF, Cambier JC. Retention of anergy and inhibition of antibody responses during acute ? herpesvirus 68 infection. J Immunol. 2012 Sep 15;189(6):2965-74. PMID: 22904300