Christina Coughlan, PhD

Professional Titles

• Senior Faculty Research Instructor

Recognitions

• Outstanding Professor of the Year Award, University of Denver. Pioneer Ambassadors, student organization (2007)
  I was totally humbled and honored to receive this award. Every year the Pioneers at the University of Denver pick 3 Faculty across the campus to receive these awards. I was one of the three
• Nominated for Faculty of the Year award, Community College of Aurora (2017)
  I was very honored to be nominated for a Faculty of the Year award while adjunct teaching Microbiology at the Community College of Aurora.

Publications

• From Gordon Conference on Plant and Microbial Cytoskeleton, August 2012: Rescuing the function of disease-causing mutant septins with alternative nucleotides. Andrew Weems, Catherine Musselman, Christina Coughlan, Courtney Johnson, Tatiana Kutateladze and Michael McMurray
• The burden of trisomy 21 disrupts the proteostasis network in Down syndrome. (2017). Aivazidis S*, Coughlan CM, Rauniyar AK, Jiang H, Liggett LA, Maclean KN, Roede JR. PLoS One. Apr 21;12(4):e0176307. doi: 10.1371/journal.pone.0176307. eCollection 2017.
• Isabel Boersma, M.S; +Jacqueline Jones, Ph.D., R.N., F.A.A.N.; Christina Coughlan, Ph.D., Julie Carter, R.N., M.N., A.N.P., David Bekelman, M.D., M.P.H., Janis Miyasaki, M.Ed., F.R.C.P.C., M.D., Jean Kutner, M.D., M.P.H., Benzi Kluger, M.D., M.S.1* J. Palliative Medicine. 2017 Sep;20(9):930-938. doi: 10.1089/jpm.2016.0325. Epub 2017 May 18.
• Aivazidis S.D., Rauniyar, A.K., Coughlan C., Jiang, H., Maclean K.N., and Roede J.R.. The burden of trisomy 21 disrupts the proteostasis network in Down syndrome. “Stress proteins and proteostasis” Gordon Research Conference, Newry, ME. 7/10 – 7/14/17.
• Coughlan, C.M., Chin-Jung Wang, A., Viltz, L.M., Chial, H.J., and Huntington Potter. Screening for Inhibitors of ApoE4-Catalyzed Aß Oligomer/Filament Formation: A Novel Approach to Alzheimer’s Disease Drug Discovery. Alzheimer's Association International Conference | July 16-20, 2017, London, England.
• Julbert Caneus, J., Granic, A., Rademakers, R., Dickson, D.W., Coughlan, C.M., Chial, H.J., and Potter, H. Abnormal Chromosome Copy Number and Associated Neuronal Cell Death in Frontotemporal Lobar Degeneration. Alzheimer's Association International Conference | July 16-20, 2017, London, England.
• Folate Prevents the Detrimental Effects of Oligomeric Aß on Insulin Receptor Localization and Function and Long-Term Potentiation. Alzheimer's Association International Conference | July 16-20, 2017, London, England.
• Caneus. J., Granic, A., Rademakers, R., Dickson, D.W, Coughlan, C.M., Chial, H.J., Potter, H. (2017) Mitotic defects lead to neuronal aneuploidy and apoptosis in frontotemporal lobar degeneration caused by MAPT mutations. Mol Biol Cell. 2017 Dec 27. pii: mbc.E17-01-0031. doi: 10.1091/mbc.E17-01-0031.
• Caneus J, Granic A, Rademakers R, Dickson DW, Coughlan CM, Chial HJ, Potter H. Mitotic defects lead to neuronal aneuploidy and apoptosis in frontotemporal lobar degeneration caused by MAPT mutations. Mol Biol Cell. 2018 Mar 1;29(5):575-586. doi: 10.1091/mbc.E17-01-0031. Epub 2017 Dec 27.
• Martin-Medina A, Lehmann M, Burgy O, Hermann S, Baarsma HA, Wagner DE, De Santis, MM, Ciolek F, Hofer TP, Frankenberger M, Aichler M, Lindner M, Gesierich W, Guenther A, Walch A, Coughlan C, Wolters P, Lee JS, Behr J, Königshoff M. Increased Extracellular Vesicles Mediate WNT-5A Signaling in Idiopathic Pulmonary Fibrosis. Am J Respir Crit Care Med. 2018 Jul 25. doi: 10.1164/rccm.201708-1580OC.
• Bubak AN, Como CN, Coughlan CM, Johnson NR, Hassell JE Jr, Mescher T, Niemeyer CS, Mahalingam R, Cohrs RJ, Boyd TD, Potter H, Russ HA, Nagel MA. Varicella Zoster Virus Infection of Primary Human Spinal Astrocytes Produces Intracellular Amylin, Amyloid-beta, and an Amyloidogenic Extracellular Environment. J Infect Dis. 2019 Oct 29. [Epub ahead of print] PubMed PMID: 31665341
• Coughlan, C., Bruce, K.D., Burgy, O., Boyd, T.D., Michel, C.R., Garcia-Perez, J.E., Adame, V., Anton, P., Bettcher, B.M., Chial, H.J., Königshoff, M., Hsieh, E.W.Y., Graner, M., Potter, H. Exosome Isolation by Ultracentrifugation and Precipitation and Techniques for Downstream Analyses. Curr Protoc Cell Biol. (2020) Sep;88(1):e110. doi: 10.1002/cpcb.110. PMID: 32633898.
• Potter, H., Woodcock, J.H., Boyd, T.D. Coughlan, C.M., O’Shaughnessy, J.R., Borges, M.T., Thaker, A.A., Raj, B.A., Adamszuk, K., Scott, D., Adame, V., Chial., H.J., Gray, H., Daniels, J., Stocker, M.E., Sillau, S.H. Safety and Efficacy of Sargramostim in the Treatment of Alzheimer’s Disease. Alzheimer’s & Dementia: Translational Research & Clinical Interventions (2021), In Press.
• Johnson, N.R, Chin-Jung Wang, A., Coughlan, C., Lucero, E.M., Viltz, L., Allen, C., Markham, N., Chial, H.J., Potter, H. Small molecule inhibitors of apolipoprotein E4-catalyzed amyloid-ß fibrillization as novel therapeutics for Alzheimer’s disease (2020), Alzheimer's & Dementia, Volume 16, Issue S2.
• Drusen proteins are released in association with exosomes. Miguel Flores-Bellver, Cui Shi, Jason Mighty, Silvia Aparicio-Domingo, Jing Zhou, Kang Li, Hannah Cobb, German Michelis, Patricia S Becerra, Andrew E Goodspeed, Søren Heissel, Christina Coughlan, Stephen Redenti and Maria Valeria Canto-Soler. Submitted 01-07-2021 to the Association for Research in Vision and Ophthalmology, Inc. (ARVO).
• Small molecule inhibitors of apolipoprotein E4-catalyzed amyloid-ß fibrillization as novel therapeutics for Alzheimer’s disease. Johnson NR, Wang A, Coughlan CM, Lucero E, Viltz L, Aghili L, Allen C, Markham N, Chial H, Potter H. Journal of Alzheimer’s & Dementia. Alzheimer’s Association International Conference (AAIC) 2020 Abstract. In press. Forthcoming
• Amylin, Aß42, and Amyloid in VZV Vasculopathy Cerebrospinal Fluid and Infected Vascular Cells.Bubak AN, Beseler C, Como CN, Coughlan CM, Johnson NR, Hassell JE, Burnet AM, Mescher T, Schmid DS, Coleman C, Mahalingam R, Cohrs RJ, Boyd TD, Potter H, Shilleh AH, Russ HA, Nagel MA. J Infect Dis. 2020 Aug 18:jiaa513. doi: 10.1093/infdis/jiaa513. Online ahead of print. PMID: 32809013
• Varicella-Zoster Virus Infection of Primary Human Spinal Astrocytes Produces Intracellular Amylin, Amyloid-ß, and an Amyloidogenic Extracellular Environment. Bubak AN, Como CN, Coughlan CM, Johnson NR, Hassell JE, Mescher T, Niemeyer CS, Mahalingam R, Cohrs RJ, Boyd TD, Potter H, Russ HA, Nagel MA.J Infect Dis. 2020 Mar 16;221(7):1088-1097. doi: 10.1093/infdis/jiz560.PMID: 31665341
• Targeting Alzheimer’s disease by inhibiting apolipoprotein E4-catalyzed amyloid-ß fibrillization. Noah R. Johnson, Athena C.-J. Wang† Christina Coughlan† Stefan Sillau1, Esteban Lucero, Lisa Viltz, Neil Markham, Cody Allen, A. Ranjitha Dhanasekaran, Heidi J. Chial and Huntington Potter.
• Potter H, Woodcock JH, Boyd TD, Coughlan CM, O'Shaughnessy JR, Borges MT, Thaker AA, Raj BA, Adamszuk K, Scott D, Adame V, Anton P, Chial HJ, Gray H, Daniels J, Stocker ME, Sillau SH. Safety and efficacy of sargramostim (GM-CSF) in the treatment of Alzheimer's disease. Alzheimers Dement (N Y). 2021;7(1):e12158. PubMed PMID: 33778150
• Bubak AN, Beseler C, Como CN, Coughlan CM, Johnson NR, Hassell JE, Burnet AM, Mescher T, Schmid DS, Coleman C, Mahalingam R, Cohrs RJ, Boyd TD, Potter H, Shilleh AH, Russ HA, Nagel MA. Amylin, Aß42, and Amyloid in Varicella Zoster Virus Vasculopathy Cerebrospinal Fluid and Infected Vascular Cells. J Infect Dis. 2021 Apr 8;223(7):1284-1294. PubMed PMID: 32809013
• Christina, Coughlan, *Jared Lindenberger, *Jeffrey, Jacot, Noah, Johnson, Paige, Anton, Shaun Bevers, Michael Graner and Huntington, Potter. Binding of Monomeric and Polymeric Alzheimer’s A? peptides to Exosomes. bioRxiv 2021.08.06.455470.doi: https://doi.org/10.1101/2021.08.06.455470
• Ted Kheng Siang Ng, Christina Coughlan, Patricia C. Heyn, Alex Tagawa, James J. Carollo, Ee Heok Kua & Rathi Mahendran. Increased plasma brain-derived neurotrophic factor (BDNF) as a biomarker for and compensatory mechanism in Mild Cognitive Impairment: A case-control study. Aging
• Md. Mahiuddin Ahmed, Noah, Johnson, Timothy Boyd, Christina Coughlan, Heidi Chial and Huntington Potter. Innate Immune System Activation and Neuroinflammation in Down Syndrome and Neurodegeneration: Therapeutic Targets or Partners? Front. Aging Neurosci., 16 September 2021 https://doi.org/10.3389/fnagi.2021.718426
• Md. Mahiuddin Ahmed, Athena Ching-Jung Wang, Mihret Elos, Heidi.J. Chial, Stefan Sillau, D. Adriana Solano, Christina Coughlan, Leila Aghili, Paige Anton, Neil Markham, Vanesa Adame, Katheleen J. Gardiner, Timothy D. Boyd, Huntington Potter. Pro-inflammatory GM-CSF Improves Learning/Memory and Brain Pathology in Dp16 Down Syndrome Mice and Improves Learning/Memory in Wild-Type Mice bioRxiv 2021.07.04.451073; doi: https://doi.org/10.1101/2021.07.04.451073
• NoahR. Johnson, Athena C.J. Wang*, Christina Coughlan*, Stefan Sillau, Esteban Lucero, LisaViltz, Neil Markham, Cody Allen, A. Ranjitha Dhanasekaran, Heidi J. Chial, Huntington Potter. Imipramine and olanzapine block apoE4-catalyzed polymerization of Aß and show evidence of improving Alzheimer’s disease cognition bioRxiv 2021.03.28.437389; doi: https://doi.org/10.1101/2021.03.28.437389
• Bubak A, Beseler C, Como CN, Coughlan CM, Johnson NR, Hassell RE, Burnet AM, Mescher, Schmid DS, Coleman C, Mahalingam R, Cohrs RJ, Boyd TD, Potter H, Shilleh AH, Russ AH, Nagel MA. Amylin, Ab42, and amyloid in VZV vasculopathy cerebrospinal fluid and infected vascular cells. Journal of Infectious Disease. 2021Apr8;223(7):1284-1294. PMID: 32809013 doi:10.1093/infdis/jiaa513.
• Brianne M. Bettcher, Kaitlin E. Olson, Nichole E. Carlson, Brice V. McConnell, Tim Boyd, Vanesa Adame, Adriana Solano, Paige Anton, Neil Markham, Ashesh Thaker, Alexandria Jensen, Erika Dallmann, Hunt Potter & Christina Coughlan. Astrogliosis and Episodic Memory in Late Life: Higher GFAP is Related to Worse Memory and White Matter Microstructure in Healthy Aging and Alzheimer’s Disease. Neurobiology of Aging . DOI: 10.1016/j.neurobiolaging.2021.02.012
• Extracellular vesicles released by human retinal pigment epithelium mediate increased polarised secretion of drusen proteins in response to AMD stressors. Miguel Flores-Bellver, Jason Mighty, Silvia Aparicio-Domingo, Kang V. Li, Cui Shi, Jing Zhou, Hannah Cobb, Patrick McGrath, GermanMichelis,Patricia Lenhart, Ganna Bilousova, Soren Heissel, Michael J. Rudy, Christina Coughlan, Andrew E. Goodspeed, S. Patricia Becerra, Stephen Redenti. M and Valeria Canto-Soler. J Extracell. Vesicles. 2021;e12165. https://doi.org/10.1002/jev2.12165
• Binding of Monomeric and Polymeric Alzheimer’s Ab peptides to Exosomes. Christina, Coughlan, *Jared Lindenberger, *Jeffrey, Jacot, Noah, Johnson, Paige, Anton, Shaun Bevers, Michael Graner and Huntington, Potter. bioRxiv 2021.08.06.455470.doi: https://doi.org/10.1101/2021.08.06.455470
• Phenotype and Neuronal Cytotoxic Function of Glioblastoma Extracellular Vesicles. Wenbo Zhou †, Daniel Lovasz †, Zoë Zizzo †, Qianbin He, Christina Coughlan, Robert G. Kowalski, Peter G. E. Kennedy, Arin N. Graner, Kevin O. Lillehei, D. Ryan Ormond, A. Samy Youssef, Michael W. Graner and Xiaoli Yu. Biomedicines 2022, 10(11), 2718; https://doi.org/10.3390/biomedicines10112718
• Zoster-associated Prothrombotic Plasma Exosomes and Increased Stroke Risk. Bubak AN, Coughlan C, Posey J, Saviola AJ, Niemeyer CS, Lewis SWR, Lopez SB, Solano A, Tyring SK, Delaney C, Neeves KB, Mahalingam R, Hansen KC, Nagel MA.J Infect Dis. 2022 Oct 6;jiac405. Doi. 10.1093/infdis/jiac405.
• Imipramine and olanzapine block apoE4-catalyzed polymerization of Aß and show evidence of improving Alzheimer’s disease cognition. Johnson, N. R., Wang, A. C. J., Coughlan, C., Sillau, S., Lucero, E., Viltz, L., Markham, N., Allen, C., Dhanasekaran, A. R., Chial, H. J., Potter, H. (2022) Alzheimers Res Ther. 2022 Jun 29;14(1):88. doi: 10.1186/s13195-022-01020-9.PMID: 35768831
• Associations of circulating insulin-growth factor-1 (IGF-1) with cognitive functions and quality of life domains in ambulatory young adults with Cerebral Palsy: a pilot study. Ted Kheng Siang Ng, Patricia C. Heyn, Alex Tagawa, Christina Coughlan and James J. Carollo. Neurology-Rehabilitation. 21st May 2022.
• The innate immune system stimulating cytokine GM-CSF improves learning/memory and interneuron and astrocyte brain pathology in Dp16 Down syndrome mice and improves learning/memory in wild-type mice. Ahmed M. M., Wang A. C., Elos, M., Chial H. J., Sillau, S., Solano, D. A., Coughlan, C., Aghili, L., Anton, P., Markham, N., Adame, V., Gardiner, K. J., Boyd, T. D., Potter. H. Neurobiol Dis. 2022 Jun 15;168:105694. doi: 10.1016/j.nbd.2022.105694. Epub 2022 Mar 18. PMID: 35307513; PMCID: PMC9045510.
• Proteasome activity modulates amyloid toxicity. Galvin J, Curran E, Arteaga F, Goossens A, Aubuchon-Endsley N, McMurray MA, Moore J, Hansen KC, Chial HJ, Potter H, Brodsky JL, Coughlan CM. FEMS Yeast Res. 2022 Mar 9;22(1):foac004. doi: 10.1093/femsyr/foac004.PMID: 35150241
• Density analysis of Enterovirus D68 shows viral 1 particles can associate with exosomes. Michael J. Rudy, Christina Coughlan, Alison M Hixon, Penny Clarke, Kenneth L Tyler Microbiol Spectr. 2022 Feb 23;10(1):e0245221. doi: 10.1128/spectrum.02452-21. Epub 2022 Feb 16.PMID: 35170992
• IGF1 deficiency integrates stunted growth and neurodegeneration in Down syndrome. Paula Araya, Kohl T. Kinning, Christina Coughlan, Jessica R. Shaw, Keith P. Smith, Ross E. Granrath, Belinda A. Enriquez-Estrada, Kayleigh Worek, Kelly D. Sullivan, Angela L. Rachubinski, Matthew D. Galbraith, Huntington Potter and Joaquin M. Espinosa. Cell Reports. Dec 2022.
• Colvett I, Saternos H, Coughlan C, Vielle A, Ledreux A. Extracellular vesicles from the CNS play pivotal roles in neuroprotection and neurodegeneration: lessons from in vitro experiments. Extracell Vesicles Circ Nucl Acids. 2023 Mar;4(1):72-89. PubMed PMID: 37859665
• Thaker AA, McConnell BV, Rogers DM, Carlson NE, Coughlan C, Jensen AM, Lopez-Paniagua D, Holden SK, Pressman PS, Pelak VS, Filley CM, Potter H, Solano DA, Heffernan KS, Bettcher BM. Astrogliosis, neuritic microstructure, and sex effects: GFAP is an indicator of neuritic orientation in women. Brain Behav Immun. 2023 Oct;113:124-135. PubMed PMID: 37394144
• Fridman V, Sillau S, Ritchie A, Bockhorst J, Coughlan C, Araya P, Espinosa JM, Smith K, Lange EM, Lange LA, Ghormli LE, Drews KL, Zeitler P, Reusch JEB. Plasma neurofilament light chain concentrations are elevated in youth-onset type 2 diabetes and associated with neuropathy. J Peripher Nerv Syst. 2023 Sep;28(3):460-470. PubMed PMID: 37341347
• Kowalski RG, Ledreux A, Violette JE, Neumann RT, Ornelas D, Yu X, Griffiths SG, Lewis S, Nash P, Monte AA, Coughlan CM, Deighan C, Grotta JC, Jones WJ, Graner MW. Rapid Activation of Neuroinflammation in Stroke: Plasma and Extracellular Vesicles Obtained on a Mobile Stroke Unit. Stroke. 2023 Mar;54(3):e52-e57. PubMed PMID: 36727508
• Bubak AN, Coughlan C, Posey J, Saviola AJ, Niemeyer CS, Lewis SWR, Lopez SB, Solano A, Tyring SK, Delaney C, Neeves KB, Mahalingam R, Hansen KC, Nagel MA. Zoster-Associated Prothrombotic Plasma Exosomes and Increased Stroke Risk. J Infect Dis. 2023 Apr 18;227(8):993-1001. PubMed PMID: 36200236
• Impaired Insulin Sensitivity Relates to Preclinical Biomarkers of Neurodegenerative Disease in Young Adults With and Without Type 1 Diabetes (T1D). Shapiro, Allison; Coughlan, Christina; Vinovskis, Carissa; Vigers, Timothy B.; Pauley, Meghan E.; Snell-Bergeon, Janet K.; Macdonald, Alexis A.; Tommerdahl, Kalie L.; Van; Raalte, Daniel; De Boer, Ian; Nelson, Robert G.; Pyle, Laura; Nadeau, Kristen J. and Bjornstad, Petter. Diabetes 2023;72(Supplement_1):1159-P. https://doi.org/10.2337/db23-1159-P
• Preclinical Biomarkers of Alzheimer's Disease Neuropathology in Young Adults with Youth-Onset Diabetes (YDM)-A Proof-of-Concept Study. Shapiro, A; Coughlan, C; Kim, J; Potter, H; Bjornstad, P; Pauley, ME; Rajic; Truong, J; Glueck, DH; Harrall, KK; Barcus, RA; Tan, J; Bettcher, B; Thaker, A; Walker, KA; Liese, AD; Dabelea, D and Whitlow, CT. Diabetes 2023;72(Supplement_1):187-LB. https://doi.org/10.2337/db23-187-LB
• Rapid Activation of Neuroinflammation in Stroke: Plasma and Extracellular Vesicles Obtained on a Mobile Stroke Unit. Robert G. Kowalski, Aurélie Ledreux, John E. Violette, Robert T. Neumann, David Ornelas, Xiaoli Yu, Steven G. Griffiths, Scott Lewis, Priscilla Nash, Andrew A. Monte, Christina M. Coughlan, Clayton Deighan, James C. Grotta, William J. Jones and Michael W. Graner. 2023; 54:e52–e57 https://doi.org/10.1161/STROKEAHA.122.041422Stroke.

Practice Locations

Specialty Information

• Basic EKG, Certificate (2015)
• IV, Certificate (2015)
• Life Coach-Strategic Intervention, Certificate (2015)
• Fertility Care Practitioner, Certificate (2009)
• Nationally Registered Advanced Emergency Medicine Technician (NRAEMT), License (2016)
• PhD Pharmacology, Other (1995)
• Assistant Research Professor, Specialized Training (2017)
• Bachelors in Science (Hons) Pharmacology and Toxicology, Specialized Training (1990)