Ganna Bilousova, PhD

Publications

• Tanneberger AE, Blomberg R, Bilousova G, Ryan AL, Magin CM. Engineered hydrogel biomaterials facilitate lung progenitor cell differentiation from induced pluripotent stem cells. Am J Physiol Lung Cell Mol Physiol. 2025 Jan 30. [Epub ahead of print] PubMed PMID: 39884665
• Inen J, Han CM, Farrel DM, Bilousova G, Kogut I. CIRCLE-Seq for Interrogation of Off-Target Gene Editing. J Vis Exp. 2024 Nov 1;(213). PubMed PMID: 39555799
• Koutsoukos SA, Bilousova G. Highlights of Gene and Cell Therapy for Epidermolysis Bullosa and Ichthyosis. Dermatol Ther (Heidelb). 2024 Sep;14(9):2379-2392. PubMed PMID: 39112824
• Neumayer G, Torkelson JL, Li S, McCarthy K, Zhen HH, Vangipuram M, Mader MM, Gebeyehu G, Jaouni TM, Jacków-Malinowska J, Rami A, Hansen C, Guo Z, Gaddam S, Tate KM, Pappalardo A, Li L, Chow GM, Roy KR, Nguyen TM, Tanabe K, McGrath PS, Cramer A, Bruckner A, Bilousova G, Roop D, Tang JY, Christiano A, Steinmetz LM, Wernig M, Oro AE. A scalable and cGMP-compatible autologous organotypic cell therapy for Dystrophic Epidermolysis Bullosa. Nat Commun. 2024 Jul 11;15(1):5834. PubMed PMID: 38992003
• Pavlova M, Balaiya V, Flores JC, Ferreyros M, Bush K, Hopkin A, Kogut I, Roop DR, Bilousova G. The Development of an Advanced Model for Multilayer Human Skin Reconstruction In Vivo. Bio Protoc. 2024 Jan 20;14(2):e4919. PubMed PMID: 38268973
• Pavlova M, Balaiya V, Flores JC, Ferreyros M, Bush K, Hopkin A, Kogut I, Roop DR, Bilousova G. The Development of an Advanced Model for Multilayer Human Skin Reconstruction In Vivo. Bio Protoc. 2024 Jan 20;14(2):e4919. PubMed PMID: 38268973
• Neumayer G, Torkelson JL, Li S, McCarthy K, Zhen HH, Vangipuram M, Jackow J, Rami A, Hansen C, Guo Z, Gaddam S, Pappalardo A, Li L, Cramer A, Roy KR, Nguyen TM, Tanabe K, McGrath PS, Bruckner A, Bilousova G, Roop D, Bailey I, Tang JY, Christiano A, Steinmetz LM, Wernig M, Oro AE. A scalable, GMP-compatible, autologous organotypic cell therapy for Dystrophic Epidermolysis Bullosa. bioRxiv. 2023 Mar 1. PubMed PMID: 36909618
• Tatavosian R, Donovan MG, Galbraith MD, Duc HN, Szwarc MM, Joshi MU, Frieman A, Bilousova G, Cao Y, Smith KP, Song K, Rachubinski AL, Andrysik Z, Espinosa JM. Cell differentiation modifies the p53 transcriptional program through a combination of gene silencing and constitutive transactivation. Cell Death Differ. 2023 Apr;30(4):952-965. PubMed PMID: 36681780
• Kizub I, Rozhok A, Bilousova G. Induced Pluripotent Stem Cells: Advances and Applications in Regenerative Medicine. In: Kitala D, Blumenberg M, editors. Possibilities and Limitations in Current Translational Stem Cell Research [Internet] London, United Kingdom: IntechOpen; 2023. Chapter 13; p.295-320. Available from: DOI: 10.5772/intechopen.109274.
• Tatavosian R, Donovan MG, Galbraith MD, Duc HN, Szwarc MM, Joshi MU, Frieman A, Bilousova G, Cao Y, Smith KP, Song K, Rachubinski AL, Andrysik Z, Espinosa JM. Cell differentiation modifies the p53 transcriptional program through a combination of gene silencing and constitutive transactivation. Cell Death Differ. 2023 Jan 21. [Epub ahead of print] PubMed PMID: 36681780
• McGarvey SS, Ferreyros M, Kogut I, Bilousova G. Differentiating Induced Pluripotent Stem Cells Toward Mesenchymal Stem/Stromal Cells. Methods Mol Biol. 2022;2549:153-167. PubMed PMID: 33772462
• Pavlova M, McGarvey SS, Bilousova G, Kogut I. A High-Efficiency Method for the Production of Endothelial Cells from Human Induced Pluripotent Stem Cells. Methods Mol Biol. 2022;2549:169-186. PubMed PMID: 33755906
• Pavlova M, McGarvey SS, Bilousova G, Kogut I. A High-Efficiency Method for the Production of Endothelial Cells from Human Induced Pluripotent Stem Cells. Methods Mol Biol. 2021 (In press)
• Flores-Bellver M, Mighty J, Aparicio-Domingo S, Li KV, Shi C, Zhou J, Cobb H, McGrath P, Michelis G, Lenhart P, Bilousova G, Heissel S, Rudy MJ, Coughlan C, Goodspeed AE, Becerra SP, Redenti S, Canto-Soler MV. Extracellular vesicles released by human retinal pigment epithelium mediate increased polarised secretion of drusen proteins in response to AMD stressors. J Extracell Vesicles. 2021 Nov;10(13):e12165. PubMed PMID: 34750957
• Warshauer EM, Brown A, Fuentes I, Shortt J, Gignoux C, Montinaro F, Metspalu M, Youssefian L, Vahidnezhad H, Jacków J, Christiano AM, Uitto J, Fajardo-Ramírez ÓR, Salas-Alanis JC, McGrath JA, Consuegra L, Rivera C, Maier PA, Runfeldt G, Behar DM, Skorecki K, Sprecher E, Palisson F, Norris DA, Bruckner AL, Kogut I, Bilousova G, Roop DR. Ancestral patterns of recessive dystrophic epidermolysis bullosa mutations in Hispanic populations suggest sephardic ancestry. Am J Med Genet A. 2021 Nov;185(11):3390-3400. PubMed PMID: 34435747
• Mermer F, Poliquin S, Rigsby K, Rastogi A, Shen W, Romero-Morales A, Nwosu G, McGrath P, Demerast S, Aoto J, Bilousova G, Lal D, Gama V, Kang JQ. Common molecular mechanisms of SLC6A1 variant-mediated neurodevelopmental disorders in astrocytes and neurons. Brain. 2021 Sep 4;144(8):2499-2512. PubMed PMID: 34028503
• McGarvey SS, Ferreyros M, Kogut I, Bilousova G. Differentiating Induced Pluripotent Stem Cells Toward Mesenchymal Stem/Stromal Cells. Methods Mol Biol. 2021 Mar 27. [Epub ahead of print] PubMed PMID: 33772462
• Pavlova M, McGarvey SS, Bilousova G, Kogut I. A High-Efficiency Method for the Production of Endothelial Cells from Human Induced Pluripotent Stem Cells. Methods Mol Biol. 2021 Mar 24. [Epub ahead of print] PubMed PMID: 33755906
• Butterfield KT, McGrath PS, Han CM, Kogut I, Bilousova G. Generation of an Induced Pluripotent Stem Cell Line with the Constitutive EGFP Reporter. Methods Mol Biol. 2020;2155:11-21. PubMed PMID: 32474864
• McGrath PS, McGarvey SS, Kogut I, Bilousova G. Efficient RNA-Based Reprogramming of Disease-Associated Primary Human Fibroblasts into Induced Pluripotent Stem Cells. Methods Mol Biol. 2020;2117:271-284. PubMed PMID: 31960386
• Diette N, Kogut I, Bilousova G. Generation of a Full-Thickness Human Skin Equivalent on an Immunodeficient Mouse. Methods Mol Biol. 2020;2109:169-183. PubMed PMID: 31119714
• McGrath PS, McGarvey SS, Kogut I, Bilousova G. Efficient RNA-Based Reprogramming of Disease-Associated Primary Human Fibroblasts into Induced Pluripotent Stem Cells. Methods Mol Biol. 2020;2117:271-284. PubMed PMID: 31960386
• Aisenbrey EA, Bilousova G, Payne K, Bryant SJ. Dynamic mechanical loading and growth factors influence chondrogenesis of induced pluripotent mesenchymal progenitor cells in a cartilage-mimetic hydrogel. Biomater Sci. 2019 Nov 19;7(12):5388-5403. PubMed PMID: 31626251
• Bilousova G. Gene Therapy for Skin Fragility Diseases: The New Generation. J Invest Dermatol. 2019 Aug;139(8):1634-1637. PubMed PMID: 31331444
• Diette N, Kogut I, Bilousova G. Generation of a Full-Thickness Human Skin Equivalent on an Immunodeficient Mouse. Methods Mol Biol. 2019 May 21. [Epub ahead of print] PubMed PMID: 31119714
• Bilousova G, DeGregori J. Elimination of unfit cells in young and ageing skin. Nature. 2019 Apr;568(7752):318-319. PubMed PMID: 30980033
• Davis-Hall D., Nguyen V., D’Ovidio T.J., Tsai E., Bilousova G. and Magin C.M. (2019). Peptide-Functionalized Hydrogels Modulate Integrin Expression and Stemness in Adult Human Epidermal Keratinocytes. Advanced Biosystems. 2019 Aug 23. doi: 10.1002/adbi.201900022
• McGrath PS, Diette N, Kogut I, Bilousova G. RNA-based Reprogramming of Human Primary Fibroblasts into Induced Pluripotent Stem Cells. J Vis Exp. 2018 Nov 26;(141). PubMed PMID: 30531717
• Shen C, Liu Y, Yu H, Gulbranson DR, Kogut I, Bilousova G, Zhang C, Stowell MHB, Shen J. The N-peptide-binding mode is critical to Munc18-1 function in synaptic exocytosis. J Biol Chem. 2018 Nov 23;293(47):18309-18317. PubMed PMID: 30275014
• Kogut I, McCarthy SM, Pavlova M, Astling DP, Chen X, Jakimenko A, Jones KL, Getahun A, Cambier JC, Pasmooij AMG, Jonkman MF, Roop DR, Bilousova G. High-efficiency RNA-based reprogramming of human primary fibroblasts. Nat Commun. 2018 Feb 21;9(1):745. PubMed PMID: 29467427
• Kogut I, Bilousova G. Environmental Influences on the Development of Epidermal Progenitors. In Reference Module in Biomedical Sciences, 2018, Elsevier, doi: 10.1016/B978-0-12-801238-3.65469-7
• Kogut I, McCarthy SM, Pavlova M, Astling DP, Chen X, Jakimenko A, Jones KL, Getahun A, Cambier JC, Pasmooij AMG, Jonkman MF, Roop DR, Bilousova G. High-efficiency RNA-based reprogramming of human primary fibroblasts. Nat Commun. 2018 Feb 21;9(1):745. PubMed PMID: 29467427
• Henry CJ, Nemkov T, Casás-Selves M, Bilousova G, Zaberezhnyy V, Higa KC, Serkova NJ, Hansen KC, D'Alessandro A, DeGregori J. Folate dietary insufficiency and folic acid supplementation similarly impair metabolism and compromise hematopoiesis. Haematologica. 2017 Dec;102(12):1985-1994. PubMed PMID: 28883079
• Hoelzl MA, Heby-Henricson K, Bilousova G, Rozell B, Kuiper RV, Kasper M, Toftgård R, Teglund S. Suppressor of Fused Plays an Important Role in Regulating Mesodermal Differentiation of Murine Embryonic Stem Cells In Vivo. Stem Cells Dev. 2015 Nov 1;24(21):2547-60. PubMed PMID: 26176320
• Kogut I, Roop DR, Bilousova G. Differentiation of Human Induced Pluripotent Stem Cells into a Keratinocyte Lineage. Methods Mol Biol. 2014 Feb 8. [Epub ahead of print] PubMed PMID: 24510784
• Bilousova, G, Roop, D.R. Induced Pluripotent Stem Cells in Dermatology: Potentials, Advances, and Limitations. Cold Spring Harb Perspect Med doi: 10.1101/cshperspect.a015164
• Bilousova G, Roop DR. Induced pluripotent stem cells in dermatology: potentials, advances, and limitations. Cold Spring Harb Perspect Med. 2014 Nov 3;4(11):a015164. PubMed PMID: 25368014
• West JD, Austin ED, Gaskill C, Marriott S, Baskir R, Bilousova G, Jean JC, Hemnes AR, Menon S, Bloodworth NC, Fessel JP, Kropski JA, Irwin D, Ware LB, Wheeler L, Hong CC, Meyrick B, Loyd JE, Bowman AB, Ess KC, Klemm DJ, Young PP, Merryman WD, Kotton D, Majka SM. Identification of a common Wnt-associated genetic signature across multiple cell types in pulmonary arterial hypertension. Am J Physiol Cell Physiol. 2014 Sep 1;307(5):C415-30. PubMed PMID: 24871858
• Kogut I, Roop DR, Bilousova G. Differentiation of human induced pluripotent stem cells into a keratinocyte lineage. Methods Mol Biol. 2014;1195:1-12. PubMed PMID: 24510784
• Bilousova G, Roop DR. Generation of functional multipotent keratinocytes from mouse induced pluripotent stem cells. Methods Mol Biol. 2013;961:337-50. PubMed PMID: 23325655
• Bilousova, G., and D.R. Roop. (2013). Generation of functional multipotent keratinocytes from mouse induced pluripotent stem cells. Methods Mol. Biol. 961:337-50
• Bilousova,G.,Chen, J., Yasuda, M. and D. R. Roop (2012). Developing a stem cell-based therapy for epidermolysis bullosa simplex by promoting an allele-specific knockout of mutant keratin 14 in induced pluripotent stem cells. 2012 Annual Meeting of the Society for Investigative Dermatology ( Raleigh, NC).
• Bilousova, G., and D.R. Roop.Generation of functional multipotent keratinocytes from mouse induced pluripotent stem cells. Methods Mol. Biol. Molecular Dermatology:Methods and Protocols (In press, 2012).
• Huebner, A.J., Zhang, L., Chen, J., Bilousova, G. and D.R. Roop (2011). Genetically Engineered Mouse Models for Keratinization Disorders: New Insights into Pathophysiology and Therapeutic Approaches. The Color Atlas of Disorders of Keratinization. H. Ogawa. Tokyo, Japan, Kyowa Kikaku, LTD: 8-16.
• Bilousova, G., Chen, J., and D.R. Roop. (2010). Differentiation of mouse induced pluripotent stem cells into a multipotent keratinocyte lineage. J Invest Dermatol. 2011 Apr;131(4):857-64. Epub 2010 Dec 9.
• Ikonomou, L., Hemnes, A.H., Bilousova, G., Hamid, R., Loyd, J.E., Hatzopoulos, A.K., Kotton, D.N., Majka, S. M. and E. D. Austin. (2011). Programmatic Change: Lung Disease Research in the Era of Induced Pluripotency. Am J Physiol Lung Cell Mol Physiol. Epub 2011 Oct 7.
• Bilousova, G. and D.R. Roop. (2010). Altering cell fate: from thymus epithelium to skin stem cells. Cell Stem Cell 7: 419-20.
• Bilousova, G., D.H. Jun, K. King, S. DeLanghe, W. Chick, E. Torchia, K. Chow, D. Klemm, D.R. Roop, and S.M. Majka. (2010). Osteoblasts derived from induced pluripotent stem cells form calcified structures in scaffolds both in vitro and in vivo. Stem Cells: Nov 23. [Epub ahead of print]