Sanchayita Mitra, MS
Sr. Research Instructor, Surgery-GI, Trauma, and Endocrine Surgery

Publications

• Thielen O, Mitra S, Debot M, Schaid T, Hallas W, Gallagher LT, Erickson C, Cralley A, Stafford P, Silliman C, D'Alessandro A, Hansen K, Sauaia A, Moore E, Mosnier L, Griffin J, Cohen M. Mitigation of trauma-induced endotheliopathy by activated protein C: A potential therapeutic for postinjury thromboinflammation. J Trauma Acute Care Surg. 2024 Jan 1;96(1):116-122. doi: 10.1097/TA.0000000000004142. Epub 2023 Sep 21. PMID: 37733304.
• Hallas WM, Mitra S, Erickson C, Thielen O, Gallagher L, Ramser B, Stocker B, Hom P, Stafford P, Moore EE, D’Alessandro A, Silliman CC, Hansen K, Cohen M. Unmitigated endoplasmic reticulum stress is implicated in the post-trauma response of the endothelium exposed to plasma from severely injured patients. Accepted to the Academic Surgical Congress 19th Annual Meeting, February 2024; Washington DC.
• Hallas WM, Mitra S, Thielen O, Gallagher L, Ramser B, Stocker B, Hom P, Erickson C, Moore EE, D’Alessandro A, Silliman CC, Hansen K, Cohen M. Plasma from severely injured trauma patients induces endothelial cell mitochondrial dysfunction. Accepted to the Western Trauma Association 53rd Annual Meeting, February 2024; Snowmass, CO.
• Hallas WM, Mitra S, Johnson K, Thielen O, Gallagher L, Ramser B, Stocker B, Hom P, Erickson C, Moore EE, D’Alessandro A, Silliman CC, Hansen K, Ravi J, Cohen M. Plasma from trauma patients induces transcriptional reprogramming of endothelial cell metabolism and inflammatory pathways. Accepted to the European Society for Trauma and Emergency Surgery 23rd European Congress, April 2024; Lisbon, Portugal.
• Cytoprotective 3K3A-Activated Protein C and Fresh Frozen Plasma as Therapies for the Endotheliopathy of Trauma Otto Thielen, Preston Stafford Margot Debot, Marguerite Kelher, Sanchayita Mitra, William Hallas, Lauren Gallagher, Terry Schaid, Benjamin Stocker, Benjamin Ramser, Angelo D’Alessandro, Kirk Hansen, Christopher Silliman, Ernest Moore, Laurent Mosnier, John Griffin: Mitchell Cohen presented at 37th EAST Annual Scientific Assembly2024, Orlando, Florida
• Gallagher LT, Erickson C, D'Alessandro A, Thielen O, Schaid T, Hallas, W, Mitra S, Stafford, P, Moore EE, Silliman CC, Calfee C S, Cohen MJ. Smoking Primes the Metabolomic Response in Trauma presented at 37th EAST Annual Scientific Assembly2024, Orlando, Florida.
• Gallagher LT, LaCroix I, Fields, AT, Mitra S, Argabright A, D'Alessandro A Erickson C, Nunez-Garcia B, Herrera-Rodriguez K Chou Y, Stocker B W, Ramser BJ, Thielen O, Hallas, W, Silliman CC, Kornblith LZ, Cohen MJ. Platelet Releasates Mitigate the Endotheliopathy of Trauma. Accepted to the Western Trauma Association 53rd Annual Meeting, February 2024; Snowmass, CO.
• Patients with Type O Blood Exhibit Distinct Multi-Omics Signature Namely Downregulation of the Lectin Complement Pathway Following Traumatic Injury Benjamin W Stocker, Ian S LaCroix, Christopher Erickson, Lauren T Gallagher, Benjamin J Ramser, Otto Thielen , William Hallas, Sanchayita Mitra, Ernest E Moore, Kirk Hansen, Angelo D’Alessandro, Christopher C Silliman, Mitchell J Cohen Accepted to the Western Trauma Association 53rd Annual Meeting, February 2024; Snowmass, CO.
• Multiomic EndoTypes Associated with Conventional Versus Viscoelastic Coagulopathy in Trauma;Ramser, B., Erickson, C., Mitra, S., Thielen, O., Gallagher, L., Hallas, W., Stocker, B., Moore, E., D’Alessandro, A., Silliman, C., Hansen, K., Cohen, M. Presented at Colorado Committee on Trauma Resident Paper Competition, ; August 25th, 2023; Colorado Springs, CO
• Ramser, B., Stocker, B., Gallagher, L., Hallas, W., Thielen, O., Erickson, C., Mitra, S., Hansen, K., D’Alessandro, A., Silliman, C., Moore, E., Cohen, M. The Multiomic Signature of Respiratory Failure After Trauma is Associated with Oxidative Stress. Abstract and to be presented at European Congress of Trauma and Emergency Surgery; April 28-30 2024; Lisbon, Portugal.
• Schaid TR Jr, Mitra S, Stafford P, DeBot M, Thielen O, Hallas W, Cralley A, Gallagher L, Jeffrey D, Hansen KC, D'Alessandro A, Silliman CC, Dabertrand F, Cohen MJ. Endothelial Cell Calcium Influx Mediates Trauma-induced Endothelial Permeability. Ann Surg. 2023 Dec 11. doi:10.1097/SLA.0000000000006164. Epub ahead of print. PMID: 38073572.
• Schaid, Terry R. Jr*; LaCroix, Ian†; Cohen, Mitchell J.*; Hansen, Kirk C.†; Moore, Ernest E.*,‡; Sauaia, Angela‡; Cralley, Alexis L.*; Thielen, Otto*; Hallas, William*; Erickson, Christopher†; Mitra, Sanchayita*; Dzieciatkowska, Monika†; Silliman, Christopher C.*,§,?; D’Alessandro, Angelo†. METABOLOMIC AND PROTEOMIC CHANGES IN TRAUMA-INDUCED HYPOCALCEMIA. Shock 60(5):p 652-663, November 2023. | DOI: 10.1097/SHK.0000000000002220
• Cralley AL, Erickson C, Schaid TR Jr, Hallas W, Thielen O, Mitra S, Stafford P, Hom P, Silliman C, Cohen MJ, Moore EE, D'Alessandro A, Hansen KC. The proteomic and metabolomic signatures of isolated and polytrauma traumatic brain injury. Am J Surg. 2023 Dec;226(6):790-797. doi: 10.1016/j.amjsurg.2023.07.040. Epub 2023 Jul 28. PMID: 37541795.
• LaCroix IS, Cralley A, Moore EE, Cendali FI, Dzieciatkowska M, Hom P, Mitra S, Cohen M, Silliman C, Sauaia A, Hansen KC, D'Alessandro A. Omics Signatures of Tissue Injury and Hemorrhagic Shock in Swine. Ann Surg. 2023 Dec 1;278(6):e1299-e1312. doi: 10.1097/SLA.0000000000005944. Epub 2023 Jun 19. PMID: 37334680; PMCID: PMC10728352.
• Coleman JR, D'Alessandro A, LaCroix I, Dzieciatkowska M, Lutz P, Mitra S, Gamboni F, Ruf W, Silliman CC, Cohen MJ. A metabolomic and proteomic analysis of pathologic hypercoagulability in traumatic brain injury patients after dura violation. J Trauma Acute Care Surg. 2023 Dec 1;95(6):925-934. doi: 10.1097/TA.0000000000004019. Epub 2023 Jul 5. PMID: 37405823.
• Pratap A, Qualman A, Garrett H, Westbrook L, The E, Mitra S, Cordero M, Monge KM, Idrovo JP, Chauhan A, Cheng L, Cohen MJ, Mungo B, Wani S, Meguid RA, McCarter MD, Meng X. Silencing Glypican-1 enhances the antitumor effects of Pictilisib via downregulating PI3K/Akt/ERK signaling in chemo-resistant esophageal adenocarcinoma. Mol Cell Oncol. 2023 Aug 15;10(1):2238873. doi: 10.1080/23723556.2023.2238873. PMID: 37649964; PMCID: PMC10464651.
• Hallas WM, Erickson C, Cohen M, Thielen O, Schaid T, Cralley A, Hom P, Mitra S, Stafford P, Sauaia A, Moore EE, Hansen K, D’Alessandro A. Toward Personalized Omics of Shock: A Potential Role for Fatty Acid Oxidation and Polyamines. Presented at the Academic Surgical Congress 18th Annual Meeting, February 2023; Houston, TX.
• Schaid, Terry & Cohen, Mitchell & D'Alessandro, Angelo & Silliman, Christopher & Moore, Ernest & Sauaia, Angela & Dzieciatkowska, Monika & Hallas, William & Thielen, Otto & DeBot, Margot & Cralley, Alexis & LaCroix, Ian & Erickson, Christopher & Mitra, Sanchayita & Banerjee, Anirban & Jones, Kenneth & Hansen, Kirk. (2022). Trauma Induces Intravascular Hemolysis, Exacerbated by RBC Transfusion and Associated with Disrupted Arginine-Nitric Oxide Metabolism. Shock. Publish Ahead of Print. 10.1097/SHK.0000000000002036.
• Schaid TR Jr, Hansen KC, Sauaia A, Moore EE, DeBot M, Cralley AL, Erickson C, Silliman CC, Banerjee A, Ghasabyan A, Jones K, Lacroix I, Mitra S, D'Alessandro A, Cohen MJ. Postinjury complement C4 activation is associated with adverse outcomes and is potentially influenced by plasma resuscitation. J Trauma Acute Care Surg. 2022 Nov 1;93(5):588-596. doi: 10.1097/TA.0000000000003713. Epub 2022 May 25. PMID: 35610738; PMCID: PMC9613483.
• Pratap A, Li A, Westbrook L, Gergen AK, Mitra S, Chauhan A, Cheng L, Weyant MJ, McCarter M, Wani S, Meguid RA, Mitchell JD, Cohen M, Fullerton D, Meng X. Glypican 1 promotes proliferation and migration in esophagogastric adenocarcinoma via activating AKT/GSK/ß-catenin pathway. J Gastrointest Oncol. 2022 Oct;13(5):2082-2104. doi: 10.21037/jgo-22-240. PMID: 36388647; PMCID: PMC9660038.
• DOI: 10.1182/blood-2020-143292
• Endothelial Cell Mediated Delay of Blood Brain Barrier Recovery Following Trauma Plasma Exposure P. Stafford, S. Mitra, M. Cohen, A. Stem, P. Lutz, P. Homs, J. Hadley1, K. Jones, A.D’Alessandro, K. Hansen, E. Moore. International Society on Thrombosis and Haemostasis ISTH 2020 July 12-14 Virtual Congress
• Isolated Tissue Injury Leads to Fibrinolytic Shutdown, TPA Resistance and Alterations in Clot Structure in a Porcine Model. NG Vigneshwar*, LR Schmitt*, EE Moore, MG Bartley, AP Eitel, JB Hadley, A Ghasabayan, S Mitra, AD Stem, PH Hom, MS Kabeil, VC Simon, M Fragoso, HB Moore, MJ Cohen, CC Silliman, JR Coleman, KC Hansen. European Congress on Trauma and Emergency Surgery, May 2020;
• Trauma Plasma Modifies Global Transcription Profiles Related To The Blood Brain Barrier J.B. Hadley, S. Mitra, D. Trahan, P. Stafford, A. Stem, P. Lutz, E.E. Moore, C. Silliman, K. Jones, M. Cohen. 16th Academic Surgical Congress February 2-24, 2021
• Stafford P‡, Mitra S‡, DeBot M, Lutz P, Stem A, Hadley J, Hom P, Cohen MJ. Astrocytes and Pericytes Attenuate Tight Junction Expression of Endothelial Cells Following Incubation with Severely Injured Patient Plasma. Plos One 2022 Jul 5;17(7):e0270817. Doi: 10.1371/journal.pone.0270817. ‡both authors contributed equally to the manuscript
• DeBot M, Mitra S, Lutz P, Schaid TR, Stafford P, Hadley JB, Hom P, Sauaia A, Silliman C, Moore EE, Cohen MJ. Shock Induces Endothelial Permeability After Trauma Through Increased Activation of RhoA GTPase. Shock (in press).
• Schaid T, LaCroix I, Cohen M, Hansen K, Moore E, Sauaia A, Cralley A, Thielen O, Dallas W, Erickson C, Mitra S, Dzieciatkowska M, Silliman C, D’Alessandro A. Metabolomic and Proteomic Changes in Trauma-induced Hypocalcemia. J Trauma Acute Care Surge (submitted).
• Schaid TR, LaCroix I, Hansen K, D’Alessandro A, Moore E, Sauaia A, Dzieciatkowska M, DeBot M, Cralley A, Thielen O, Hallas W, Erickson C, Mitra S, Banerjee A, Jones K, Silliman C, Cohen M. A Proteomic Analysis of NETosis in Trauma: Emergence of SerpinB1 as a Key Player. J Trauma Acute Care Surg (in press).
• Schaid TR, LaCroix I, Hansen K, D’Alessandro A, Moore E, Sauaia A, Dzieciatkowska M, DeBot M, Cralley A, Thielen O, Hallas W, Erickson C, Mitra S, Banerjee A, Jones K, Silliman C, Cohen M. A Proteomic Analysis of NETosis in Trauma: Emergence of SerpinB1 as a Key Player. J Trauma Acute Care Surg (in press).
• Cralley, A. L., Moore, E. E., Coleman, J. R., Vigneshwar, N., Bartley, M., Kissau, D., Eitel, A., Hom, P., Mitra, S., Ghasabyan, A., Fragoso, M., Guo, Z., Deguchi, H., Griffin, J. H., Cohen, M. J., Silliman, C. C., Banerjee, A., Hansen, K., & Sauaia, A. Hemorrhagic shock and tissue injury provoke distinct components of trauma-induced coagulopathy in a swine model. [published online ahead of print, 2022 Nov 1]. Eur J Trauma Emerg Surg.
• Cralley AL, Moore EE, Fox CJ, Kissau D, DeBot M, Schaid TR, Mitra S, Hom P, Fragoso M, Ghasabyan A, Erickson C, D'Alessandro A, Hansen KC, Cohen MJ, Silliman CC, Sauaia A. Zone 1 REBOA in a combat DCBI swine model does not worsen brain injury. Surgery. 2022 Jun 8:S0039-6060(22)00327-0. doi: 10.1016/j.surg.2022.04.055. Online ahead of print.PMID: 35690490
• William Hallas, Christopher Erickson, Mitchell Cohen, Otto Thielen, Terry R. Schaid, Alexis L. Cralley, Patrick Hom, Sanchayita Mitra, Preston Stafford, Angela Sauaia, Ernest E. Moore, Kirk Hansen, Angelo D’Alessandro. Toward Personalized Omics of Shock: A Potential Role for Fatty Acid Oxidation and Polyamines. Accepted to Academic Surgical Congress 18th Annual Meeting, February 2023; Houston, TX.
• DeBot M, Sumislawski J, Mitra S, Stem A, Cohen MJ. Response to Resuscitation after Injury Attenuates Endothelial Barrier Permeability and Correlates with Clinical Outcomes. 44th Annual Conference on Shock; virtual; 2021; Poster
• DeBot M, Mitra S, Lutz P, Hadley JP, Silliman C, Stafford P, Cohen MJ. Shock Increases Endothelial Permeability after Traumatic Brain Injury. American College of Surgeons Clinical Congress 2021, Owen H. Wangensteen Scientific Forum; virtual; quick-shot. 8th Annual University of Colorado Department of Surgery Research Symposium; 2021; virtual; oral presentation
• Schaid T, Cohen M, Erickson C, Hansen K, Moore E, Sauaia A, Cralley A, LaCroix I, Thielen O, Hallas W, Mitra S, Dzieciatkowska M, Silliman C, D’Alessandro A. Obesity Influences the Metabolic and Proteomic Signatures of Trauma. 22nd European Congress of Trauma and Emergency Surgery. Ljubljana, Slovenia; 2023; Accepted for oral presentation.
• Schaid T, Mitra S, Stafford P, Moore E, Sauaia A, DeBot M, Thielen O, Hallas W, Cralley A, LaCroix I, Erickson C, Chandler J, Hansen K, D’Alessandro A, Silliman C, Cohen M. Endothelial Cell Calcium Influx Mediates Trauma-induced Endothelial Permeability. American College of Surgeons Committee on Trauma Resident Paper Competition; 2022; 1st place basic science abstract in Region 8 competition.
• Schaid T, Cohen M, DeBot M, Moore E, Sauaia A, Silliman C, Hansen K, LaCroix I, Erickson C, Cralley A, Hallas M, Thielen O, Mitra S, Banerjee A, Jones K, D’Alessandro A. S1P is Decreased in Post-injury ARDS and Repletion may Mitigate Metabolopathy of Hemorrhagic Shock. 18th Annual Academic Surgical Congress; 2023; Houston, TX; Accepted for oral presentation.
• Schaid T, Lacroix I, Hansen K, Moore EE, DeBot M, Silliman C, Sauaia A, , Cralley A, Erickson C, Banerjee A, Jones K, Mitra S, Ghasabyan A, Cohen MJ, D’Alessandro A. Plasma Polyamines are Early Predictors of Adverse Outcomes Following Trauma. 45th Annual Conference on Shock; Toronto, ON; 2022; Poster.
• Schaid, T., Mitra, S., DeBot, M., Moore, E., Dabertrand F, Cohen, M. Calcium Signaling in Trauma: Increased Intracellular Calcium Flux in the Severely Injured. Experimental Biology 2022; Philadelphia, PA; 2022; Oral and Poster.
• Cralley, A., Erikcons, C., Schaid, T., Thielen, O., Hallas, W., Mitra, S., Hom, P., Silliman, C., D’Alessandro, A., Sauaia, A., Cohen, M., Moore, E., Hansen, K. Traumatic Brain Injury Endotypes: Polytrauma TBI Results in Distinct Pathway Activation Compared to Isolated TBI. Submitted to Southwesthern Surgical Conference. April 2023.
• Cralley, A., LaCroix, I., Moore, E., Schaid, T., Thielen, O., Hallas, W., Mitra, S., Hom, P., Cohen, M Silliman, C., Hansen, K., D’Alessandro, A., Sauaia, A. Zone 1 Aortic Occlusion Provokes Mitochondrial Metabolic Disturbances while Zone 3 Attenuates Metabolite Buildup. Submitted to European Conference Trauma and Emergency Surgery. April 2023.
• Cralley, A., Moore, E., Schaid, T., Thielen, O., Hallas, W., Hom, P., Mitra, S., Kelher, M., Fragoso, M., Hansen, K., Cohen, M Silliman, C., Fox, C., Sauaia, A. Reperfusion Induced Coagulopathy: REBOA Contribution to the Development of Coagulopathy in Severely Injured Patients. Accepted for Oral. 8th Annual Academic Surgical Congress. Feb 9 2023
• Cralley AL, Moore EE, Fox CJ, Kissau D, DeBot M, Schaid TR, Mitra S, Hom P, Fragoso M, Ghasabyan A, Erickson C, D'Alessandro A, Hansen KC, Cohen MJ, Silliman CC, Sauaia A. Zone 1 REBOA In A Combat DCBI Swine Model Does Not Increase Risk Of Secondary Brain Injury. Academic Surgical Congress (podium). Orlando, FL. February 3 2022.
• Cralley, A., Moore, E., DeBot, M., Schaid, T., Fox, C., Cohen, M., Hansen, K., Ghasabyan, A., Fragoso,M., Mitra, S., Hom, P., Silliman, C., Sauaia, A. Total Thrombus Formation Analysis System (T-TAS) Detects Poor Clot Formation in a Swine Model of Polytrauma and Hemorrhagic Shock. Congress of the International Society on Thrombosis and Haemostasis (poster). London, England. July 11 2022.
• Cralley, A., Moore, E., DeBot, M., Schaid, T., Fox, C., Cohen, M., Hansen, K., Ghasabyan, A., Fragoso,M., Mitra, S., Hom, P., Silliman, C., Sauaia, A. EtCO2: A Shockingly Poor Clinical Indicator Of Metabolic Status During Aortic Occlusion. 45th Annual Conference on Shock (poster). Toronto, Canada. June 6 2022.
• William Hallas, David Nerguizian, Otto Thielen, Terry R. Schaid, Margot DeBot, Alexis L. Cralley, Caitlin Robinson, Patrick Hom, Sanchayita Mitra, Preston Stafford, Ernest E. Moore, Kirk Hansen, Christopher Silliman, Angela Sauaia, Angelo D’Alessandro, Mitchell Cohen. The effect of the post trauma milieu on endothelial metabolism: Ex-vivo trauma plasma from severely injured and shocked patients causes oxidative stress, mitochondrial dysfunction, and fatty acid oxidation deficiency in human endothelial cells. Accepted to the European Society for Trauma and Emergency Surgery 22nd European Congress, May 2023; Ljubljana, Slovenia.
• William Hallas, Sanchayita Mitra, Daniel Stephenson, Otto Thielen, Terry R. Schaid, Margot DeBot, Alexis L. Cralley, Caitlin Robinson, Patrick Hom, Preston Stafford, Ernest E. Moore, Kirk Hansen, Christopher Silliman, Angela Sauaia, Angelo D’Alessandro, Mitchell Cohen. Ex-vivo trauma plasma from severely injured patients causes early oxylipin anti-inflammatory signaling followed by a state of oxidative stress in human endothelial cells. Accepted to the Southwestern Surgical Congress 74th Scientific Meeting, April 2023; Ojai Valley Inn, CA.
• ON Thielen, M Debot, S Mitra, TJ Schaid, WM Hallas, C Erickson, P Stafford, A Cralley, C Silliman, A D’Alessandro, K Hansen, A Sauaia, EE Moore, L Mosnier, J Griffin, MJ Cohen Mitigation of Trauma Induced Endotheliopathy by Activated Protein C: A Potential Therapeutic for Post-Injury Thromboinflammation Western Trauma Association 2023
• O Thielen, S Mitra, P Stafford, T Schaid, W Hallas, A Cralley, M Debot, C Silliman, A D’Alessandro, K Hansen, A Sauaia, E Moore, L Mosnier, J Griffin, M Cohen Mitigation of Trauma-Induced Endotheliopathy by Activated Protein C: A potential therapeutic for post-injury thrombosis and inflammation American College of Surgeons Committee on Trauma 2023
• O Thielen, C Erickson, TJ Schaid, WM Hallas, A Cralley, S Mitra, P Stafford, C Silliman, A Sauaia, A D’Alessandro, K Hansen, EE Moore, MJ Cohen. Biochemical Significance of PH in Trauma-Induced Coagulopathy. 22nd European Congress of Trauma and Emergency Surgery. Ljubljana, Slovenia; 2023