Brian Freed, PhD, MA, MS

Professional Titles

• Executive Director, ClinImmune Cell and Gene Therapy

Recognitions

• Ph.D. Research and Teaching Award, CU Department of Medicine (2007)
  

Research Interests

Publications

• Lambert C, McCue J, Portas M, Ouyang Y, Li J, Rosano TG, Lazis A, Freed BM. Acrolein in cigarette smoke inhibits T cell activation. J Allergy Clin Immunology 116:916, 2005.
• Bill JR, Mack DG, Falta MT, Maier LA, Sullivan AK, Joslin FG, Martin AK, Freed BM, Kotzin BL, Fontenot AP. Beryllium presentation to CD4+ T cells is dependent on a single amino acid residue of the MHC class II ß chain. J Immunology 175:7029, 2005.
• Yang Y, Aubrey M, Zhang G, Ji Y, Freed BM. HLA-B*1586 is a novel hybrid HLA-H15/B22 allele with unique serology and haplotypic associations. Tissue Antigens 67:176, 2006.
• Meyer MA, Dwyer-Nield LD, Hurteau G, Keith RL, Ouyang Y, Freed BM, Kisley LR, Geraci MW, Bonventre JV, Nemenoff RA, Malkinson AM. Attenuation of the inflammatory response following butylated hydroxytoluene (BHT) treatment of cytosolic phospholipase A2 (cPLA2) null mice. Am J Physiol Lung Cell Mol Physiol , 2006..
• Frazer-Abel A, McCue J, Lazis S, Portas M, Lambert C, Freed BM. Cigarette tar phenols impede T cell cycle progression by inhibiting cyclin-dependent kinases. Mol Immunol (in press).
• Gibney EM, Cagle L, Freed B, Warnell SE, Chan L, Wiseman AC. Detection of donor-specific antibodies using HLA-coated microspheres: another tool for kidney transplant risk stratification. Nephrology Dialysis and Transplantation (in press).
• Freed BM, Abbott B. Immunology of Cord Blood Transplantation. Clin Applied Immunol Review (solicited review by editor)
• Frazer-Abel A, McCue J, Lazis S, Portas M, Lambert C, Freed BM. Cigarette tar phenols impede T cell cycle progression by inhibiting cyclin-dependent kinases. Mol Immunol 44:488, 2007.
• Meyer MA, Dwyer-Nield LD, Hurteau G, Keith RL, Ouyang Y, Freed BM, Kisley LR, Geraci MW, Bonventre JV, Nemenoff RA, Malkinson AM. Attenuation of the inflammatory response following butylated hydroxytoluene treatment of cytosolic phospholipase A2 null mice. Am J Physiol Lung Cell Mol Physiol 290:2160, 2006.
• Gibney EM, Cagle L, Freed B, Warnell SE, Chan L, Wiseman AC. Detection of donor-specific antibodies using HLA-coated microspheres: another tool for kidney transplant risk stratification. Nephrology Dialysis and Transplantation 21:2625, 2006.
• Frazer-Abel A, McCue J, Lazis S, Portas M, Lambert C, Freed BM. Cigarette tar phenols impede T cell cycle progression by inhibiting cyclin-dependent kinases. Mol Immunol 44:488, 2007.
• Lambert C, Li J, Jonscher K, Quintana M, Harvey JA, Freed BM. Acrolein inhibits cytokine gene expression by alkylating cysteine and arginine residues in the NF-?B1 DNA-binding domain. J Biol Chem 282:19666, 2007.
• Mack CL, Falta ML, Sullivan AK, Karrer F, Sokol RJ, Freed BM, Fontenot AP. Oligoclonal expansions of CD4+ and CD8+ T cells in the target organ of patients with biliary atresia. Gastroenterology 133:278, 2007.
• Regulation of transcription by immunotoxicants. Comprehensive Toxicology (in press).
• Freed BM, Lambert C. Redox regulation of transcription by cigarette smoke. In Comprehensive Toxicology (in press).
• Freed BM, Lambert C. Redox regulation of transcription by cigarette smoke. In Comprehensive Toxicology 5.18:351-359, 2010.
• Dreskin SC, Tripputi MT, Aubrey MT, Mustafa SS, Atkins D, Leo HL, Song B, Schlichting DG, Talwar H, Wang Q, Freed BM. Peanut-allergic subjects and their peanut-tolerant siblings have large differences in peanut-specific IgG that are independent of HLA class II. Clin Immunology 137:366-373, 2010.
• Shang S, Henao-Tamayo M, Bai X, Oberley-Deegan R, Shanley C, Orme IM, Minor M, Lambert C, Freed BM, Iseman MD, Martin RJ, Basaraba RJ, Ordway D, Chan ED. Cigarette smoke exposure increases susceptibility to tuberculosis-evidence from in vivo and in vitro infection models. J Infect Disease (in press).
• Lang J, Weiss N, Freed BM, Torres RM, Pelanda R. Generation of hematopoietic humanized mice in the newborn Balb/c Rag2nullIL2r?null mouse model: a multivatiable optimization approach. Cell Immunology (submitted).
• Lang J, Weiss N, Freed BM, Torres RM, Pelanda R. Generation of hematopoietic humanized mice in the newborn Balb/c Rag2nullIL2r?null mouse model: a multivariable optimization approach. Cell Immunology 140102, 2011.
• Gutman JA, Miller S, Kuenne S, Oppenheim J, Quinones R, Freed BM, Zarlengo G. Cord blood collection after cesarean section improves banking efficiency. Transfusion 51:2050, 2011.
• Freed BM, Schuyler RP, Aubrey MT. Association of the HLA-DRB1 epitope LA67,74 with rheumatoid arthritis and citrullinated vimentin binding. Arthritis Rheum 63:3733, 2011.
• Kamani NR, Walters MC, Carter S, Aquino V, Brochstein JA, Chaudhury S, Eapen M, Freed BM, Grimley M, Levine JE, Logan B, Moore T, Panepinto J, Parikh S, Pulsipher MA, Sande J, Schultz KR, Spellman S, Shenoy S. Unrelated donor cord blood transplantation for children with severe sickle cell disease: results of one cohort from the phase II study from the Blood and Marrow Transplant clinical trials network. Biol Blood Marrow Transplant (in press), 2012.
• Kamani NR, Walters MC, Carter S, Aquino V, Brochstein JA, Chaudhury S, Eapen M, Freed BM, Grimley M, Levine JE, Logan B, Moore T, Panepinto J, Parikh S, Pulsipher MA, Sande J, Schultz KR, Spellman S, Shenoy S. Unrelated donor cord blood transplantation for children with severe sickle cell disease: results of one cohort from the phase II study from the Blood and Marrow Transplant clinical trials network. Biol Blood Marrow Transplant 18:1265, 2012.
• Menezes CA, Sullivan AK, Falta MT, Freed BM, Rocha MO, Gollub KJ, Fontenot AP, Dutra WO. Highly conserved CDR3 region in circulating CD4(+)Vß5(+) T cells may be associated with cytotoxic activity in Chagas disease. Clin Exp Immunol 169:109, 2012.
• Rocha V Spellman S, Zhang MJ, Ruggeri A, Purtill D, Brady C, Altamuro D, Baxter-Lowe L, Beaudoux E, Beddard RL, Bergamaschi P, Chow R, Freed B, Koegler G, Kurtzberg J, Larghero J, Lecchi L, Mrowiec Z, Nagler A, Navarette C, Prasad V, Prasath A, Price T, Pouthier F, Ratanatharathorn V, Sanders J, Sender L, van Rood JJ, Horowitz MM, Gluckman E, Eapen M. Effect of HLA-matching recipients to donor non-inherited maternal antigens on outcomes after mismatched umbilical cord blood transplantation for hematologic malignancies. Biol Blood Marrow Transplant 18:1890, 2012.
• Lang J, Kelly M, Freed BM, McCarter MD, Kedl RM, Torres RM, Pelanda R. Studies of lymphocyte reconstitution in a humanized mouse model reveal a requirement of T cells for human B cell maturation. J Immunol 190:2090, 2013.
• Mack CL, Anderson KM, Aubrey MT, Rosenthal P, Sokol RJ, Freed BM. Lack of HLA predominance and HLA shared epitopes in biliary Atresia. Springerplus. 2013 Dec;2(1):42. PubMed PMID: 23505615
• Lang J, Kelly M, Freed BM, McCarter MD, Kedl RM, Torres RM, Pelanda R. Studies of lymphocyte reconstitution in a humanized mouse model reveal a requirement of T cells for human B cell maturation. J Immunol. 2013 Mar 1;190(5):2090-101. PubMed PMID: 23335750
• Choudhary R, Baturin D, Fosmire S, Freed B, Porter CC. Knockdown of HPRT for selection of genetically modified human progenitor cells. PLoS One 8(3):e59594, 2013.
• Andersen NJ, Mondal TK, Preissler MT, Freed BM, Stockinger S, Bell E, Druschel C, Buck Louis GM, Lawrence DA. Detection of immunoglobulin isotypes from dried blood spots. J Immunol Methods http://dx.doi.org/10.1016/j.jim.2013.12.001, 2013.
• Schade H, Sen S, Neff CP, Freed BM, Gao D, Gutman JA, Palmer BE. Programmed Death 1 Expression on CD4( ) T Cells Predicts Mortality after Allogeneic Stem Cell Transplantation. Biol Blood Marrow Transplant. 2016 Dec;22(12):2172-2179. PubMed PMID: 27519280
• Roark CL, Anderson KM, Aubrey MT, Rosloniec EF, Freed BM. Arthritogenic peptide binding to DRB1*01 alleles correlates with susceptibility to rheumatoid arthritis. J Autoimmun. 2016 Aug;72:25-32. PubMed PMID: 27143337
• Anderson KM, Stastny TH, Freed BM. Reply. Arthritis Rheumatol. 2016 Aug;68(8):2051-2. PubMed PMID: 27110851
• Anderson KM, Roark CL, Portas M, Aubrey MT, Rosloniec EF, Freed BM. A Molecular Analysis of the Shared Epitope Hypothesis: Binding of Arthritogenic Peptides to DRB1*04 Alleles. Arthritis Rheumatol. 2016 Jul;68(7):1627-36. PubMed PMID: 26866513
• Lang J, Ota T, Kelly M, Strauch P, Freed BM, Torres RM, Nemazee D, Pelanda R. Receptor editing and genetic variability in human autoreactive B cells. J Exp Med. 2016 Jan 11;213(1):93-108. PubMed PMID: 26694971
• Lower tacrolimus exposure and time in therapeutic range increase the risk of de novo donor-specific antibodies in the first year of kidney transplantation. Davis S, Gralla J, Klem P, Tong S, Wedermyer G, Freed B, Wiseman A, Cooper JE. Am J Transplant. 2017 Sep 19. doi: 10.1111/ajt.14504. [Epub ahead of print] PMID: 28925597
• Replacing mouse BAFF with human BAFF does not improve B-cell maturation in hematopoietic humanized mice. Lang J, Zhang B, Kelly M, Peterson JN, Barbee J, Freed BM, Di Santo JP, Matsuda JL, Torres RM, Pelanda R. Blood Adv. 2017 Dec 19;1(27):2729-2741. doi: 10.1182/bloodadvances.2017010090. eCollection 2017 Dec 26. PMID: 29296925
• Adebiyi OO, Gralla J, Klem P, Freed B, Davis S, Wiseman A, Cooper JE. Clinical Significance of Pre-Transplant Donor Specific Antibodies in the Setting of Negative Cell-Based Flow Cytometry Crossmatching in Kidney Transplant Recipients. Am J Transplant. 2016 May 3. [Epub ahead of print] PubMed PMID: 27140940
• Brady C, Armitage S, Freed B, Duffy M, Gass A, Spellman S, Kurtzberg J, Regan D. How transplant centers deal with the dextran shortage: recommendations for comparing alternatives. Transfusion. 2016 Sep 5. [Epub ahead of print] PubMed PMID: 27596242
• Selecting the Best Donor for Haploidentical Transplant: Impact of HLA, Killer Cell Immunoglobulin-Like Receptor Genotyping, and Other Clinical Variables. Solomon SR, Aubrey MT, Zhang X, Piluso A, Freed BM, Brown S, Jackson KC, Morris LE, Holland HK, Solh MM, Bashey A. Biol Blood Marrow Transplant. 2018 Apr;24(4):789-798. doi: 10.1016/j.bbmt.2018.01.013. Epub 2018 Jan 31. PMID: 29355721
• Lower tacrolimus exposure and time in therapeutic range increase the risk of de novo donor-specific antibodies in the first year of kidney transplantation. Davis S, Gralla J, Klem P, Tong S, Wedermyer G, Freed B, Wiseman A, Cooper JE. Am J Transplant. 2018 Apr;18(4):907-915. doi: 10.1111/ajt.14504. Epub 2017 Oct 24. PMID: 28925597
• Epstein-Barr Virus Type 2 Infects T Cells and Induces B Cell Lymphomagenesis in Humanized Mice. Coleman CB, Lang J, Sweet LA, Smith NA, Freed BM, Pan Z, Haverkos B, Pelanda R, Rochford R. J Virol. 2018 Oct 12;92(21). pii: e00813-18. doi: 10.1128/JVI.00813-18. Print 2018 Nov 1. PMID: 30089703
• Capasso A, Lang J, Pitts TM, Jordan KR, Lieu CH, Davis SL, Diamond JR, Kopetz S, Barbee J, Peterson J, Freed BM, Yacob BW, Bagby SM, Messersmith WA, Slansky JE, Pelanda R, Eckhardt. Characterization of immune responses to anti-PD-1 mono and combination immunotherapy in hematopoietic humanized mice implanted with tumor xenografts. J Immunother Cancer 7:37, 2019.
• Anderson K, Carey B, Martin A, Roark C, Chalk C, Nowell-Bostic M, Freed B, Aubrey M, Trapnell B, Fontenot A. Pulmonary alveolar proteinosis: An autoimmune disease lacking an HLA association. PLos One 14(3):e0213179, 2019.
• Zhang Y, Anderson KM, Freed BM, Dai S, Pacheco KA. HLA-DR53 (DRB4*01) associates with nickel sensitization. Ann Allergy Asthma Immunol. 2020 Nov;125(5):614-616. PubMed PMID: 32693207
• Lang J, Capasso A, Jordan KR, French JD, Kar A, Bagby SM, Barbee J, Yacob BW, Head LS, Tompkins KD, Freed BM, Somerset H, Clark TJ, Pitts TM, Messersmith WA, Eckhardt SG, Wierman ME, Leong S, Kiseljak-Vassiliades K. Development of an Adrenocortical Cancer Humanized Mouse Model to Characterize Anti-PD1 Effects on Tumor Microenvironment. J Clin Endocrinol Metab. 2020 Jan 1;105(1). PubMed PMID: 31513709
• Stites E, Renner B, Laskowski J, Le Quintrec M, You Z, Freed B, Cooper J, Jalal D, Thurman JM. Complement fragments are biomarkers of antibody-mediated endothelial injury. Mol Immunol. 2020 Feb;118:142-152. PubMed PMID: 31884386
• Alves da Costa T, Peterson JN, Lang J, Shulman J, Liang X, Freed BM, Boackle SA, Lauzurica P, Torres RM, Pelanda R. Central human B cell tolerance manifests with a distinctive cell phenotype and is enforced via CXCR4 signaling in hu-mice. Proc Natl Acad Sci U S A. 2021 Apr 20;118(16). PubMed PMID: 33850015
• Bai X, Hippensteel J, Leavitt A, Maloney JP, Beckham D, Garcia C, Li Q, Freed BM, Ordway D, Sandhaus RA, Chan ED. Hypothesis: Alpha-1-antitrypsin is a promising treatment option for COVID-19. Med Hypotheses. 2021 Jan;146:110394. PubMed PMID: 33239231
• Marín-Jiménez JA, Capasso A, Lewis MS, Bagby SM, Hartman SJ, Shulman J, Navarro NM, Yu H, Rivard CJ, Wang X, Barkow JC, Geng D, Kar A, Yingst A, Tufa DM, Dolan JT, Blatchford PJ, Freed BM, Torres RM, Davila E, Slansky JE, Pelanda R, Eckhardt SG, Messersmith WA, Diamond JR, Lieu CH, Verneris MR, Wang JH, Kiseljak-Vassiliades K, Pitts TM, Lang J. Testing Cancer Immunotherapy in a Human Immune System Mouse Model: Correlating Treatment Responses to Human Chimerism, Therapeutic Variables and Immune Cell Phenotypes. Front Immunol. 2021;12:607282. PubMed PMID: 33854497
• Bai X, Buckle AM, Vladar EK, Janoff EN, Khare R, Ordway D, Beckham D, Fornis LB, Majluf-Cruz A, Fugit RV, Freed BM, Kim S, Sandhaus RA, Chan ED. Enoxaparin augments alpha-1-antitrypsin inhibition of TMPRSS2, a promising drug combination against COVID-19. Sci Rep. 2022 Mar 25;12(1):5207. doi: 10.1038/s41598-022-09133-9. https://doi.org/10.1038/s41598-022-09133-9
• Roark CL, Ho BE, Aubrey MT, Anobile C, Israeli S, Phang TL, Braxton D, Ho AP, Freed BM. HLA homozygosity is associated with non-Hodgkin lymphoma. Human Immunol 83:730, 2022 https://doi.org/10.1016/j.humimm.2022.08.002
• Solomon SR, Aubrey MT, Zhang X, Jackson KC, Roark CL, Freed BM, Morris LE, Holland HK, Bashey A, Solh MM. Lineage-specific relapse prediction following haploidentical transplant with posttransplant cyclophosphamide based on recipient HLA-B-leader genotype and HLA-C-Group KIR ligand. Transplantation Cellular Therapy 2022 https://doi.org/10.1016/j.jtct.2022.06.023
• Lang J, Leal AD, Jiménez JAM, Hartman SJ, Shulman J, Navarro N, Lewis M, Capasso A, Bagby S, Yacob BW, MacBeth M, Freed BM, Eckhardt SG, Jordan K, Blatchford PJ, Pelanda R, Lieu C, Messersmith WA, Pitts TM. Cabozantinib sensitizes microsatellite stable colorectal cancer to immune checkpoint blockade by immune modulation in human immune system mouse models. Frontiers Oncology 2022 https://doi.org/10.3389/fonc.2022.877635
• Laskowitz DT, Troy J, Poehlein E, Bennett ER, Sphall EJ, Wingard JR, Freed B, Belagaje SR, Khanna A, Jones W, Volpi JJ, Marrotte E, Kurtzburg J. A randomized, placebo-controlled, Phase II trial of intravenous allogeneic non-HLA matched, unrelated donor, umbilical cord blood infusion for ischemic stroke. Stem Cell Translational Medicine 2023.
• Bai X, Verma D, Garcia C, Musheyev A, Kim K, Fornis L, Griffith DE, Li L, Whittel N, Gadwa J, Ohanjanyan T, Eggleston MJ, Galvan M, Freed BM, Ordway D, Chan ED. Ex vivo and in vivo evidence that cigarette smoke-exposed T regulatory cells impair host immunity against Mycobacterium tuberculosis. Frontiers Cell Infection Microbiology 13:1216492. https://doi.org/10.1101/2022.01.20.477097

Professional Memberships

• American Society for Histocompatibility and Immunogenetics, Member
• American Society of Transplantation, Member
• American Association of Immunologists, Member
• American Society for Blood and Marrow Transplantation, Member
• The Donor Alliance Medical Advisory Council, Member

Practice Locations

• University of Colorado Hospital
• Presbyterian/St. Luke's Medical Center
• Children's Hospital Colorado

Specialty Information

• Allergy and Immunology
• Histocompatibility and Immunogenetics, Board Certification (0001)
• Fellow American College of Histocompatibility and Immunogenetics, Board Certification (2021)