Dwight Klemm, PhD

Professor, Medicine-Pulmonary Sciences & Critical Care


Publications

  • Kosmoski, L.A., H.L. Miller and D.J. Klemm. In Combination, Nucleoside Reverse Transcriptase Inhibitors Have Significant Effects on 3T3-L1 Adipocyte Lipid Accumulation and Survival. Antiviral Therapy. Accepted for Publication January 2006, In Press.
  • Crossno, J.T., Jr., K.G. Morris and D.J. Klemm. 2005. Attenuation of hypoxia-induced pulmonary artery remodeling by a peroxisome proliferator-activated receptor-gamma agonist. Chest 128:580S.
  • Gerasimovskaya EV, Tucker DA, Weiser-Evans M, Wenzlau JM, Klemm DJ, Banks M, Stenmark KR. 2004. Extracellular ATP-induced proliferation of adventitial fibroblasts requires phosphoinositide 3-kinase, Akt, mammalian target of rapamycin, and p70 S6 kinase signaling pathways. J Biol Chem. 2005 Jan 21;280(3):1838-48
  • Crossno Jr J, Garat C, Reusch J, Morris K, Dempsey EC, McMurtry IF, Stenmark KR, Klemm DJ. Rosiglitazone Attenuates Hypoxia-Induced Pulmonary Arterial Remodeling. Am J Physiol Lung Cell Mol Physiol. 2006 Dec 22; [Epub ahead of print]
  • Fox KE, Fankell DM, Erickson PF, Majka SM, Crossno JT Jr, Klemm DJ. Depletion of cAMP-response element-binding protein/ATF1 inhibits adipogenic conversion of 3T3-L1 cells ectopically expressing CCAAT/enhancer-binding protein (C/EBP) alpha, C/EBP beta, or PPAR gamma 2. J Biol Chem. 2006 Dec 29;281(52):40341-53. Epub 2006 Oct 27.
  • Crossno JT Jr, Majka SM, Grazia T, Gill RG, Klemm DJ. Rosiglitazone promotes development of a novel adipocyte population from bone marrow-derived circulating progenitor cells. J Clin Invest. 2006 Dec;116(12):3220-8.
  • Garat CV, Fankell D, Erickson PF, Reusch JE, Bauer NN, McMurtry IF, Klemm DJ. Platelet-derived growth factor BB induces nuclear export and proteasomal degradation of CREB via phosphatidylinositol 3-kinase/Akt signaling in pulmonary artery smooth muscle cells. Mol Cell Biol. 2006 Jul;26(13):4934-48.
  • Kosmiski LA, Miller HL, Klemm DJ. In combination, nucleoside reverse transcriptase inhibitors have significant effects on 3T3-L1 adipocyte lipid accumulation and survival. Antivir Ther. 2006;11(2):187-95.
  • Crossno, J.T., J.E.B. Reusch, K.G. Morris, E.C. Dempsey, I.F. McMurtry, K.R. Stenmark, and D.J. Klemm. 2007. Rosiglitazone Attenuates Hypoxia-Induced Pulmonary Arterial Remodeling. Am. J. Physiol. Lung Cellular Mol. Physiol. 293:L885-L897
  • Schroeder-Gloeckner, J.M., S.M. Rahman, R.C. Jannsen, L. Qiao, M. Roper, S.J. Fisher, E. Lowe, D.J. Orlicky, J.L. McManaman, C. Palmer, W.L. Gitomer, W. Huang, R.M. O'Doherty, T.C. Becker, D.J. Klemm, D.R. Jensen, L.K. Pulawa, R.H. Eckel, and J.E. Friedman. 2007. CCAAT/Enhancer Binding Protein beta Deletion Reduces Adiposity, Hepatic Steatosis and Diabetes in Leprdb/db Mice. J. Biol. Chem. 282:15717-15729
  • Irwin, D., K. Helm, N. Campbell, M. Inamura, K.A. Fagan, J. Harral, M. Carr, K.A. Young, D.J. Klemm, S.A. Gebb, E.C. Dempsey, J. West, and S.M. Majka. 2007. Neonatal Lung Side Population Cells Demonstrate Endothelial Potential and are Altered in Response to Hyperoxia-Induced Lung Simplification. Am. J. Physiol. Lung Cell. Mol. Physiol. 293:L941-L951
  • Majka, S.M., K.E. Fox, J.C. Psilas, K.M. Helm, C.R. Childs, A.S. Acosta, M.V. Garcia, B.T. Woessner, T.R. Shade and D.J. Klemm. 2010. De Novo Generation of White Adipocytes from the Myeloid Lineage Via Mesenchymal Intermediates is Age, Adipose Depot and Gender Specific. Proceeding of the National Academy of Science USA. Under review.
  • Klemm, D.J., J.T. Crossno, K. Morris, J.E.B. Reusch and C.V. Garat. 2010. The Superoxide Dismutase Mimetic, Tempol, Decreases Pulmonary Artery Remodeling and Smooth Muscle Cell CREB Depletion in Rats Exposed to Chronic Hypoxia. Biochemical Journal. Under review.
  • Van Rheen, Z., C.L. Fattman, S. Domarski, S. Majka, D. Klemm, K.R. Stenmark and E. Nozik-Grayck. 2010. Lung EC-SOD Overexpression Attenuates Bleomycin-Induced Pulmonary Hypertension and Pulmonary Vascular Remodeling. American Journal of Respiratory and Critical Care Medicine. Accepted pending revision.
  • Watson, P.A., J.E.B. Reusch, D.J. Klemm, S. McCune, L.A. Leinwand, S.W. Luckey, D.A. Brown and R.L. Moore. 2010. Restoration of CREB Function is Linked to Completion and Stabilization of Limited Duration, Adaptive Cardiac Hypertrophy in Response to Exercise. Am J Physiol: Heart Circ Physiol. Accepted pending revision.
  • Garat, C.V., J.T. Crossno, Jr., T.M. Sullivan, J.E.B. Reusch and D.J. Klemm. 2010. Thiazolidinediones Prevent PDGF-BB-Induced CREB Depletion in Pulmonary Artery Smooth Muscle Cells by Preventing Upregulation of Casein Kinase 2a’ Catalytic Subunit. Journal of Cardiovascular Pharmacology. Accepted January 2010.
  • Schauer, I.E., L.A. Knaub, M. Lloyd, P.A. Watson, C. Gliwa, K.E. Lewis, A. Chait, D.J. Klemm, J.M. Gunter, R. Bouchard, T.O. McDonald, K.D. O’Brien and J.E.B. Reusch. 2010. CREB Downregulation in Vascular Disease: A Common Response to Cardiovascular Risk. Arteriosclerosis, Thrombosis and Vascular Biology. Accepted January 2010.
  • Takeda, K., M. Okamoto, S. De Langhe, E. Dill, M. Armstrong, N. Reisdorf, D. Irwin, M. Koster, J. Wilder, K. Stenamrk, J. West, D. Klemm, E. Gelfand, E. Nozik-Grayck and S. Majka. 2009. Peroxisome Proliferator Activated Receptor-? Agonist Treatment Increases Septation and Angiogenesis and Decreases Airway Hyperresponsiveness in a Murine Model of Neonatal Chronic Lung Disease. Anatomical Record 292:1045-1061.
  • Schauer, I.E., L.A. Knaub, M. Lloyd, P.A. Watson, C. Gliwa, K.E. Lewis, A. Chait, D.J. Klemm, J.M. Gunter, R. Bouchard, T.O. McDonald, K.D. O’Brien and J.E.B. Reusch. 2010. CREB Downregulation in Vascular Disease: A Common Response to Cardiovascular Risk. Arteriosclerosis, Thrombosis and Vascular Biology. 30:733-741.
  • Garat, C.V., J.T. Crossno, Jr., T.M. Sullivan, J.E.B. Reusch and D.J. Klemm. 2010. Thiazolidinediones Prevent PDGF-BB-Induced CREB Depletion in Pulmonary Artery Smooth Muscle Cells by Preventing Upregulation of Casein Kinase 2a’ Catalytic Subunit. Journal of Cardiovascular Pharmacology. 55:469-480.
  • Van Rheen, Z., C.L. Fattman, S. Domarski, S. Majka, D. Klemm, K.R. Stenmark and E. Nozik-Grayck. 2010. Lung EC-SOD Overexpression Attenuates Bleomycin-Induced Pulmonary Hypertension and Pulmonary Vascular Remodeling. American Journal of Respiratory and Critical Care Medicine. Accepted June 2010.
  • Majka, S.M., K.E. Fox, J.C. Psilas, K.M. Helm, C.R. Childs, A.S. Acosta, M.V. Garcia, B.T. Woessner, T.R. Shade and D.J. Klemm. 2010. De Novo Generation of White Adipocytes from the Myeloid Lineage Via Mesenchymal Intermediates is Age, Adipose Depot and Gender Specific. Proceeding of the National Academy of Science USA. 107:14781-14786.
  • Bilousova, G., D. Hyun Jun, K.B. King, S. DeLanghe, W.S. Chick, E.C. Torchia, K.S. Chow, D.J. Klemm, D.R. Roop and S.M. Majka. 2010. Osteoblasts Derived from Induced Pluripotent Stem Cells Form Calcified Structures in Scaffolds both In Vivo and In Vitro. Stem Cells. Accepted November 2010.
  • Klemm, D.J., J.T. Crossno, K. Morris, J.E.B. Reusch and C.V. Garat. 2010. The Superoxide Dismutase Mimetic, Tempol, Decreases Pulmonary Artery Remodeling and Smooth Muscle Cell CREB Depletion in Rats Exposed to Chronic Hypoxia. Journal of Cardiovascular Pharmacology. Accepted December 2010.
  • Jun, D., C. Garat, J. West, N. Thorn, K. Chow, T. Cleaver, T. Sullivan, E.C. Torchia, C. Childs, T. Shade, M. Tadjali, A. Lara, E. Nozik-Grayck, S. Malkowski, B. Sorrentino, B. Meyrick, D. Klemm, M. Rojas, D.H. Wagner, Jr., and S.M. Majka. 2011. The Pathology of Bleomycin-Induced Fibrosis is Associated with Loss of Resident Lung Mesenchymal Stem Cells Which Regulate Effector T-Cell Proliferation. Stem Cells. Accepted January 2011.
  • Majka, S.M., Y. Barak and D.J. Klemm. 2011. Adipocyte Origins: Weighing the Possibilities. Stem Cells 29:1034-1040.
  • McCurdy, C.E., S. Schenk, M.J. Holliday, D.J. Klemm, S.M. Majka and J.E. Friedman. 2011. Up-Regulation of the p55a/p50a Subunits of PI 3-Kinase Link Obesity-Induced Insulin Resistance with Adipose Tissue Macrophage Recruitment. Diabetes. Accepted January 2012.
  • Klemm, D.J.. S.M. Majka, J.T. Crossno, Jr., J.C. Psilas, J.E. Reusch and C.V. Garat. 2011. Reduction of Reactive Oxygen Species Prevents Hypoia-Induced CREB Depletion in Pulmonary Artery Smooth Muscle Cells. Journal of Cardiovascular Pharmacology 58:181-191.
  • Chow, K., Joshua Fessel, K. Ihida-Stansbury, E. Schmidt, D. Alvarez, B. Graham, D. Harrison, D. Wagner, E. Nozik-Grayck, J. West, D. Klemm, and S. Majka. 2012. Dysfunctional Resident Lung Mesenchymal Cell Contribute to Pulmonary Microvascular Remodleing. Pulmonary Circulation. Accepted January 2013.
  • McCurdy, C.E., S. Schenk, M.J. Holliday, A. Philp, J.A. Houck, D. Patsouris, P.S. MacLean, S.M. Majka, D.J. Klemm and J.E. Friedman. 2013. Attenuated Pik3r1 Expression Prevents Insulin Resistance and Adipose Tissue Macrophage Accumulation in Diet-Induced Obese Mice. Diabetes 61: 2495-2505.
  • Majka, S.M., H.L. Miller, T. Sullivan, P.F. Erickson, R. Kong, M. Weiser-Evans, R. Nemenoff, R. Moldovan, S.A. Morandi, J.A. Davis and D.J. Klemm. 2012. Adipose Lineage Specification of Bone Marrow-Derived Myeloid Cells. Adipocyte 1:215-229.
  • Majka SM, Miller HL, Helm KM, Acosta AS, Childs CR, Kong R, Klemm DJ. Analysis and isolation of adipocytes by flow cytometry. Methods Enzymol. 2014;537:281-96. PubMed PMID: 24480352
  • Garat CV, Crossno JT Jr, Sullivan TM, Reusch JE, Klemm DJ. Inhibition of phosphatidylinositol 3-kinase/Akt signaling attenuates hypoxia-induced pulmonary artery remodeling and suppresses CREB depletion in arterial smooth muscle cells. J Cardiovasc Pharmacol. 2013 Dec;62(6):539-48. PubMed PMID: 24084215
  • Keller AC, Knaub LA, Miller MW, Birdsey N, Klemm DJ, Reusch JE. Saxagliptin Restores Vascular Mitochondrial Exercise Response in the Goto-Kakizaki Rat. J Cardiovasc Pharmacol. 2014 Sep 26. [Epub ahead of print] PubMed PMID: 25264749
  • McCurdy CE, Klemm DJ. Adipose tissue insulin sensitivity and macrophage recruitment: Does PI3K pick the pathway?. Adipocyte. 2013 Jul 1;2(3):135-42. PubMed PMID: 23991359
  • Marriott S, Baskir RS, Gaskill C, Menon S, Carrier EJ, Williams J, Talati M, Helm K, Alford CE, Kropski JA, Loyd J, Wheeler L, Johnson J, Austin E, Nozik-Grayck E, Meyrick B, West JD, Klemm DJ, Majka SM. ABCG2pos lung mesenchymal stem cells are a novel pericyte subpopulation that contributes to fibrotic remodeling. Am J Physiol Cell Physiol. 2014 Oct 15;307(8):C684-98. PubMed PMID: 25122876
  • West JD, Austin ED, Gaskill C, Marriott S, Baskir R, Bilousova G, Jean JC, Hemnes AR, Menon S, Bloodworth NC, Fessel JP, Kropski JA, Irwin D, Ware LB, Wheeler L, Hong CC, Meyrick B, Loyd JE, Bowman AB, Ess KC, Klemm DJ, Young PP, Merryman WD, Kotton D, Majka SM. Identification of a common Wnt-associated genetic signature across multiple cell types in pulmonary arterial hypertension. Am J Physiol Cell Physiol. 2014 Sep 1;307(5):C415-30. PubMed PMID: 24871858
  • Geary K, Knaub LA, Schauer IE, Keller AC, Watson PA, Miller MW, Garat CV, Nadeau KJ, Cree-Green M, Pugazhenthi S, Regensteiner JG, Klemm DJ, Reusch JE. Targeting mitochondria to restore failed adaptation to exercise in diabetes. Biochem Soc Trans. 2014 Apr;42(2):231-8. PubMed PMID: 24646223
  • Majka SM, Miller HL, Helm KM, Acosta AS, Childs CR, Kong R, Klemm DJ. Analysis and isolation of adipocytes by flow cytometry. Methods Enzymol. 2014;537:281-96. PubMed PMID: 24480352
  • Chow K, Fessel JP, Kaoriihida-Stansbury, Schmidt EP, Gaskill C, Alvarez D, Graham B, Harrison DG, Wagner DH Jr, Nozik-Grayck E, West JD, Klemm DJ, Majka SM. Dysfunctional resident lung mesenchymal stem cells contribute to pulmonary microvascular remodeling. Pulm Circ. 2013 Jan;3(1):31-49. PubMed PMID: 23662173
  • Gavin KM, Gutman JA, Kohrt WM, Wei Q, Shea KL, Miller HL, Sullivan TM, Erickson PF, Helm KM, Acosta AS, Childs CR, Musselwhite E, Varella-Garcia M, Kelly K, Majka SM, Klemm DJ. De novo generation of adipocytes from circulating progenitor cells in mouse and human adipose tissue. FASEB J. 2015 Nov 18. [Epub ahead of print] PubMed PMID: 26581599
  • Keller AC, Knaub LA, Miller MW, Birdsey N, Klemm DJ, Reusch JE. Saxagliptin restores vascular mitochondrial exercise response in the Goto-Kakizaki rat. J Cardiovasc Pharmacol. 2015 Feb;65(2):137-47. PubMed PMID: 25264749
  • Obesity-related pulmonary arterial hypertension in rats correlates with increased circulating inflammatory cytokines and lipids and with oxidant damage in the arterial wall but not with hypoxia. Irwin DC, Garat CV, Crossno JT Jr, MacLean PS, Sullivan TM, Erickson PF, Jackman MR, Harral JW, Reusch JE, Klemm DJ. Pulm Circ. 2015 Dec;4(4):638-53. doi: 10.1086/678510.
  • Keller, A., L. Knaub, P. McClatchey, C. Connon, R. J. Bouchard, M. Miller, K. Geary, L. Walker, D. Klemm and J.E.B. Reusch. 2016. Differential Mitochondrial Adaptation in Primary Vascular Smooth Muscle Cells from a Diabetic Rat Model. Oxidative Med Cellular Longevity In press.
  • Gaskill CF, Carrier EJ, Kropski JA, Bloodworth NC, Menon S, Foronjy RF, Taketo MM, Hong CC, Austin ED, West JD, Means AL, Loyd JE, Merryman WD, Hemnes AR, De Langhe S, Blackwell TS, Klemm DJ, Majka SM. Disruption of lineage specification in adult pulmonary mesenchymal progenitor cells promotes microvascular dysfunction. J Clin Invest. 2017 Jun 1;127(6):2262-2276. PubMed PMID: 28463231
  • Gavin KM, Majka SM, Kohrt WM, Miller HL, Sullivan TM, Klemm DJ. Hematopoietic-to-mesenchymal transition of adipose tissue macrophages is regulated by integrin ß1 and fabricated fibrin matrices. Adipocyte. 2017 Jul 3;6(3):234-249. PubMed PMID: 28441086
  • Gavin KM, Kohrt WM, Klemm DJ, Melanson EL. Modulation of Energy Expenditure by Estrogens and Exercise in Women. Exerc Sport Sci Rev. 2018 Oct;46(4):232-239. PubMed PMID: 30001272
  • Gavin KM, Sullivan TM, Kohrt WM, Majka SM, Klemm DJ. Ovarian Hormones Regulate the Production of Adipocytes From Bone Marrow-Derived Cells. Front Endocrinol (Lausanne). 2018;9:276. PubMed PMID: 29892267
  • Gaskill CF, Carrier EJ, Kropski JA, Bloodworth NC, Menon S, Foronjy RF, Taketo MM, Hong CC, Austin ED, West JD, Means AL, Loyd JE, Merryman WD, Hemnes AR, De Langhe S, Blackwell TS, Klemm DJ, Majka SM. Disruption of lineage specification in adult pulmonary mesenchymal progenitor cells promotes microvascular dysfunction. J Clin Invest. 2017 Jun 1;127(6):2262-2276. PubMed PMID: 28463231
  • Gavin KM, Majka SM, Kohrt WM, Miller HL, Sullivan TM, Klemm DJ. Hematopoietic-to-mesenchymal transition of adipose tissue macrophages is regulated by integrin ß1 and fabricated fibrin matrices. Adipocyte. 2017 Jul 3;6(3):234-249. PubMed PMID: 28441086
  • Gewin LS, Summers ME, Harral JW, Gaskill CF, Khodo SN, Neelisetty S, Sullivan TM, Hopp K, Reese JJ, Klemm DJ, Kon V, Ess KC, Shi W, Majka SM. Inactivation of Tsc2 in Abcg2 lineage-derived cells drives the appearance of polycystic lesions and fibrosis in the adult kidney. Am J Physiol Renal Physiol. 2019 Nov 1;317(5):F1201-F1210. PubMed PMID: 31461347
  • Garat CV, Majka SM, Sullivan TS Crossno JT, RDOI: 10.1177eusch JEB, Klemm DJ. CREB Depletion in Smooth Muscle Cells Promotes Medial Thickening, Adventitial Fibrosis and Elicits Pulmonary Hypertension. Pulm Circ DOI:10.1177/2045894019898374
  • Summers ME, Richmond BW, Menon S, Sheridan RM, Kropski JA, Majka SA, Taketo MM, Bastarache JA, West JD, De Langhe S, Geraghty P, Klemm DJ, Chu HW, Friedman RS, Tao YK, Foronjy RF, Majka SM. Resident mesenchymal vascular progenitors modulate adaptive angiogenesis and pulmonary remodeling via regulation of canonical Wnt signaling. FASEB J. 2020 Aug;34(8):10267-10285. PubMed PMID: 32533805
  • Garat CV, Majka SM, Sullivan TM, Crossno JT Jr, Reusch JEB, Klemm DJ. CREB depletion in smooth muscle cells promotes medial thickening, adventitial fibrosis and elicits pulmonary hypertension. Pulm Circ. 2020 Apr-Jun;10(2):2045894019898374. PubMed PMID: 32313640
  • Strassheim D, Sullivan T, Irwin DC, Gerasimovskaya E, Lahm T, Klemm DJ, Dempsey EC, Stenmark KR, Karoor V. Metabolite G-Protein Coupled Receptors in Cardio-Metabolic Diseases. Cells. 2021 Nov 29;10(12). PubMed PMID: 34943862
  • Gavin KM, Sullivan TM, Maltzahn JK, Rahkola JT, Acosta AS, Kohrt WM, Majka SM, Klemm DJ. Hematopoietic stem cells produce intermediate lineage adipocyte progenitors that simultaneously express both myeloid and mesenchymal lineage markers in adipose tissue. Adipocyte. 2021 Dec;10(1):394-407. PubMed PMID: 34404315
  • Gavin KM, Sullivan TM, Maltzahn JK, Jackman MR, Libby AE, MacLean PS, Kohrt WM, Majka SM, Klemm DJ. Hematopoietic Stem Cell-Derived Adipocytes Modulate Adipose Tissue Cellularity, Leptin Production and Insulin Responsiveness in Female Mice. Front Endocrinol (Lausanne). 2022;13:844877. PubMed PMID: 35721743
  • Libby AE, Solt M, Jackman M, Sherk R, Foright G, Johnson TT, Nguyen M, Breit N, Hulett M, Rudolph E, Wellberg P, Roberson P, Jambal R, Scalzo J, Higgins TR, Kumar M, Weirman Z, Pan K, Shankar D, Klem D, Moreau K, Kohrt W,MacLean P. 2024. Effects of Follicle Stimulating Hormone on Energy Balance and Tissue Metabolic Health After Loss of Ovarian Function. Amer J Endocrinol Metab. Accepted Jam 2024.