Robert Sclafani, PhD

Research Interests

Publications

• Chien, C.-Yi , Chen, Bo-R., Chou, C.-K, Sclafani, R.A., Su, J.-Y. The yeast Cdc8 exhibits both deoxythymidine monophosphate and diphosphate kinase activities. FEBS Lett 583, 2281-2286 (2009). PMID: 19540237
• . Roy, S., Gu, M., Ramasamy, K., Singh, R.P., Agarwal, C., Siriwardana, S., Sclafani, R.A. and Agarwal, R. p21/Cip1 and P27/Kip1 are Essential Molecular Targets of Inositol Hexaphosphate for Its Antitumor Efficacy against Prostate Cancer. Cancer Research 69, 1166-73 (2009) PMID: 19176374 PMCID: PMC2633420
• Holzen, T.M. and Sclafani, R. A., and. Genetic interaction of RAD53 protein kinase with histones is important for DNA replication (2010) Cell Cycle 9:4735-47.
• Kaur, M. Tyagi,A., Singh, R.P., Sclafani, R.A., Agarwal, R. and Agarwal, C., Grape seed extract up-regulates p21 (Cip1) by redox-mediated activation of ERK1/2 and post-transcriptional mechanisms for its cell cycle arrest effect in colon carcinoma HT29 cells. (2011) Mol. Carcinogenesis, in press
• Radhakrishnan, S., Reddivari, L., Sclafani, R., Das, U.N., and Vanamala, J. Resveratrol potentiates grape seed extract induced human colon cancer cell apoptosis. (2011) Frontiers in Bioscience E3, 1509-1523.
• Kaur, M. Tyagi,A., Singh, R.P., Sclafani, R.A., Agarwal, R. and Agarwal, C., Grape seed extract up-regulates p21 (Cip1) by redox-mediated activation of ERK1/2 and post-transcriptional mechanisms for its cell cycle arrest effect in colon carcinoma HT29 cells. (2011) Mol Carcinog 50: 553-62.
• Tyagi, A., Gu, M., Takahata, T., Frederick, B., Agarwal, C., Siriwardana, S., Agarwal, R., and Sclafani, R.A. (2011). Resveratrol Selectively Induces DNA Damage, Independent of Smad4 Expression, in Its Efficacy against Human Head and Neck Squamous Cell Carcinoma. Clin Cancer Res 17, 5402-5411.
• Han, K.J., Foster, D.G., Zhang, N.Y., Kanisha, K., Dzieciatkowska, M., Sclafani, R.A., Hansen, K.C., Peng, J., and Liu, C.W. (2012). Ubiquitin-specific protease 9x deubiquitinates and stabilizes the spinal muscular atrophy protein-survival motor neuron. J Biol Chem 287, 43741-43752. PMID: 23112048 [PubMed - in process] PMCID: PMC3527959 [Available on 2013/12/21]
• Zhong, Y., Nellimoottil, T. Peace, J.M., Knott, S. R.V., Villwock, S.K., Yee, J.M., Jancuska, J.M., Rege, S. Tecklenburg, M., Sclafani, R.A., Tavaré, S., Aparicio, O.M. (2013) The level of origin firing inversely affects the rate of replication fork progression. J. Cell Biol., in press
• Karim Bahmed, Curtis Henry, Michael Holliday, Jasmina S Redzic, Madalina Ciobanu, Fengli Zhang, Colin Weekes, Robert A Sclafani, James DeGregori and Elan Z Eisenmesser. Extracellular cyclophilin-A stimulates ERK1/2 phosphorylation in a cell-dependent manner but broadly stimulates nuclear factor kappa B. (2012) Cancer Cell Int. 20:19.
• Shrotriya S, Tyagi A, Deep G, Orlicky DJ, Wisell J, Wang XJ, Sclafani RA, Agarwal R, Agarwal C. Grape seed extract and resveratrol prevent 4-nitroquinoline 1-oxide induced oral tumorigenesis in mice by modulating AMPK activation and associated biological responses. Mol Carcinog. 2013 Nov 14. [Epub ahead of print] PubMed PMID: 24243690
• Zhong Y, Nellimoottil T, Peace JM, Knott SR, Villwock SK, Yee JM, Jancuska JM, Rege S, Tecklenburg M, Sclafani RA, Tavaré S, Aparicio OM. The level of origin firing inversely affects the rate of replication fork progression. J Cell Biol. 2013 Apr 29;201(3):373-83. PubMed PMID: 23629964
• Ramey CJ, Sclafani RA. Functional Conservation of the Pre-Sensor One Beta-finger Hairpin (PS1-hp) Structures in MCM Proteins of Saccharomyces cerevisiae and Archaea. G3 (Bethesda). 2014. Epub 2014/05/31. doi: 10.1534/g3.114.011668. PubMed PMID: 24875627.
• An X, Zhang C, Sclafani RA, Seligman P, Huang M. The late-annotated small ORF LSO1 is a target gene of the iron regulon of Saccharomyces cerevisiae. Microbiologyopen. 2015 Oct 8. [Epub ahead of print] PubMed PMID: 26450372
• Shrotriya S, Agarwal R, Sclafani RA. A perspective on chemoprevention by resveratrol in head and neck squamous cell carcinoma. Adv Exp Med Biol. 2015;815:333-48. PubMed PMID: 25427916
• Brandao LN, Ferguson R, Santoro I, Jinks-Robertson S, Sclafani RA. The Role of Dbf4-Dependent Protein Kinase in DNA Polymerase zeta-Dependent Mutagenesis in Saccharomyces cerevisiae. Genetics. 2014. Epub 2014/05/31. doi: 10.1534/genetics.114.165308. PubMed PMID: 24875188.
• Shrotriya S, Tyagi A, Deep G, Orlicky DJ, Wisell J, Wang XJ, Sclafani RA, Agarwal R, Agarwal C. Grape seed extract and resveratrol prevent 4-nitroquinoline 1-oxide induced oral tumorigenesis in mice by modulating AMPK activation and associated biological responses. Mol Carcinog. 2015 Apr;54(4):291-300. PubMed PMID: 24243690
• Brandão LN, Ferguson R, Santoro I, Jinks-Robertson S, Sclafani RA. The role of Dbf4-dependent protein kinase in DNA polymerase ?-dependent mutagenesis in Saccharomyces cerevisiae. Genetics. 2014 Aug;197(4):1111-22. PubMed PMID: 24875188
• Shrotriya S, Agarwal R, Sclafani R. A Perspective on Chemoprevention by Resveratrol in Head and Neck Squamous Cell Carcinoma. In: Vasiliou V, Zakhari S, Seitz HK, Hoek JB, editors. Biological Basis of Alcohol-Induced Cancer: Springer International Publishing; 2015. p. 333-48.
• Ramey CJ, Sclafani RA. Functional conservation of the pre-sensor one beta-finger hairpin (PS1-hp) structures in mini-chromosome maintenance proteins of Saccharomyces cerevisiae and archaea. G3 (Bethesda). 2014 May 28;4(7):1319-26. PubMed PMID: 24875627
• Daniel Rossbach and Robert A. Sclafani. Role of DDK in replication initiation In “The Initiation of DNA Replication in Eukaryotes”; ed. Daniel Kaplan; Springer Publishing
• Todd MC, Langan TA, Sclafani RA. Doxycycline-Regulated p16MTS1 Expression Suppresses the Anchorage-Independence and Tumorigenicity of Breast Cancer Cell Lines that Lack Endogenous p16. Journal of Cancer. 2016. Epub in press.
• Rossbach D, Sclafani RA. Role of DDK in replication initiation. In: Kaplan DL, editor. The Initiation of DNA Replication in Eukaryotes. 1 ed. New York: Springer International Publishing; 2016. p. XII, 563.
• Sclafani RA, Hesselberth JR. O Cdc7 kinase where art thou?. Curr Genet. 2017 Nov 13. [Epub ahead of print] PubMed PMID: 29134273
• Todd MC, Langan TA, Sclafani RA. Doxycycline-Regulated p16(MTS1) Expression Suppresses the Anchorage-Independence and Tumorigenicity of Breast Cancer Cell Lines that Lack Endogenous p16. J Cancer. 2017;8(2):190-198. PubMed PMID: 28243323
• Rossbach D, Bryan DS, Hesselberth JR, Sclafani R. Localization of Cdc7 Protein Kinase During DNA Replication in Saccharomyces cerevisiae. G3 (Bethesda). 2017. Epub 2017/09/20. doi: 10.1534/g3.117.300223. PubMed PMID: 28924058.

Professional Memberships

• American Society for Microbiology (ASM), Fellow
• The Genetics Society of America (GSA), Member
• American Association for the Advancement of Science (AAAS), Member
• Society for Advancing Chicanos/Hispanics and Native Americans in Science (SACNAS), Member
• American Association for Cancer Research (AACR), member
• American Society for Cell Biology (ASCB), Member

Practice Locations