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Graduate School :
Graduate School :
- PhD, University of Colorado - Boulder (2004)
- BS, Colorado School of Mines (CO) (1997)
Professional Titles
- Associate Professor
- Bioengineering
Research Interests
My research interest is in development of optical technologies for biomedical applications. My lab is developing is flexible fiber- optic implantable miniature microscopes using two-photon microscopy. The applications of these devices include better understanding of the function of brain or peripheral networks by imaging and photo-stimulating neurons rapidly in a full 3D volume. The goal is to study brain networks in freely moving animals undergoing complex behavior tasks. Additionally, I have been working under the NIH SPARC initiative to develop these miniature microscopes to image and photo-stimulate axons in the vagus nerve through a nerve cuff. Another application is use of the miniature microscope for label-free imaging in endoscopy applications. Other work in my lab includes development of a custom superresolution STimulated Emission Depletion (STED) microscope to image live cells at sub-100 nm resolution at video rates to visualize dynamics of protein localization or structural changes in vivo.
Publications
- Fontaine AK, Futia GL, Rajendran PS, Littich SF, Mizoguchi N, Shivkumar K, Ardell JL, Restrepo D, Caldwell JH, Gibson EA, Weir RFF. Optical vagus nerve modulation of heart and respiration via heart-injected retrograde AAV. Sci Rep. 2021 Feb 11;11(1):3664. PubMed PMID: 33574459
- Ma M, Futia GL, de Souza FMS, Ozbay BN, Llano I, Gibson EA, Restrepo D. Molecular layer interneurons in the cerebellum encode for valence in associative learning. Nat Commun. 2020 Aug 31;11(1):4217. PubMed PMID: 32868778
- Heffernan BM, Meyer SA, Restrepo D, Siemens ME, Gibson EA, Gopinath JT. A Fiber-Coupled Stimulated Emission Depletion Microscope for Bend-Insensitive Through-Fiber Imaging. Sci Rep. 2019 Jul 31;9(1):11137. PubMed PMID: 31366899
- Ozbay BN, Futia GL, Ma M, Bright VM, Gopinath JT, Hughes EG, Restrepo D, Gibson EA. Three dimensional two-photon brain imaging in freely moving mice using a miniature fiber coupled microscope with active axial-scanning. Sci Rep. 2018 May 25;8(1):8108. PubMed PMID: 29802371
- Futia GL, Schlaepfer IR, Qamar L, Behbakht K, Gibson EA. Statistical performance of image cytometry for DNA, lipids, cytokeratin, & CD45 in a model system for circulation tumor cell detection. Cytometry A. 2017 Jul;91(7):662-674. PubMed PMID: 28608985
- Niederriter RD, Ozbay BN, Futia GL, Gibson EA, Gopinath JT. Compact diode laser source for multiphoton biological imaging. Biomed Opt Express. 2017 Jan 1;8(1):315-322. PubMed PMID: 28101420
- Zohrabi M, Cormack RH, Mccullough C, Supekar OD, Gibson EA, Bright VM, Gopinath JT. Numerical analysis of wavefront aberration correction using multielectrode electrowetting-based devices. Opt Express. 2017 Dec 11;25(25):31451-31461. PubMed PMID: 29245820
- Supekar OD, Ozbay BN, Zohrabi M, Nystrom PD, Futia GL, Restrepo D, Gibson EA, Gopinath JT, Bright VM. Two-photon laser scanning microscopy with electrowetting-based prism scanning. Biomed Opt Express. 2017 Dec 1;8(12):5412-5426. PubMed PMID: 29296477
- Soltys JN, Meyer SA, Schumann H, Gibson EA, Restrepo D, Bennett JL. Determining the Spatial Relationship of Membrane-Bound Aquaporin-4 Autoantibodies by STED Nanoscopy. Biophys J. 2017 Apr 25;112(8):1692-1702. PubMed PMID: 28445760
- Fontaine AK, Gibson EA, Caldwell JH, Weir RF. Optical Read-out of Neural Activity in Mammalian Peripheral Axons: Calcium Signaling at Nodes of Ranvier. Sci Rep. 2017 Jul 18;7(1):4744. PubMed PMID: 28720792
- Meyer SA, Ozbay BN, Potcoava M, Salcedo E, Restrepo D, Gibson EA. Super-resolution imaging of ciliary microdomains in isolated olfactory sensory neurons using a custom two-color stimulated emission depletion microscope. J Biomed Opt. 2016 Jun 1;21(6):66017. PubMed PMID: 27367253
- Ozbay BN, Losacco JT, Cormack R, Weir R, Bright VM, Gopinath JT, Restrepo D, Gibson EA. Miniaturized fiber-coupled confocal fluorescence microscope with an electrowetting variable focus lens using no moving parts. Opt Lett. 2015 Jun 1;40(11):2553-6. PubMed PMID: 26030555
- Potcoava MC, Futia GL, Aughenbaugh J, Schlaepfer IR, Gibson EA. Raman and coherent anti-Stokes Raman scattering microscopy studies of changes in lipid content and composition in hormone-treated breast and prostate cancer cells. J Biomed Opt. 2014;19(11):111605. PubMed PMID: 24933682
General Information
Graduate Schools:
- PhD, University of Colorado - Boulder (2004)
Undergraduate Schools:
- BS, Colorado School of Mines (CO) (1997)
Department: University of Colorado - Denver