Rytis Prekeris, PhD

Publications

• Jing, J., Junutula. J.R., Wu, C., Burden, J., Peden, A.A., and R. Prekeris (2010) FIP1/RCP binding to Golgin-97 regulates retrograde transport from recycling endosomes to Trans-Golgi Network. Molecular Biology of the Cell. 21(17):3041-3053.
• Szperl, A.M., Golachowna, M.R., Bruinenberg, M., Prekeris, R., Thunnissen, W.H., Hoekstra, D., Wijmenga, C., Ksiazyk, J., Rings, E.M., Wapenaar, M.C., and S.C.D. van IJzendoorn (2011) Functional characterization of mutations in the myosin Vb gene associated with microvillus inclusion disease. Journal of Pediatric Gasteroenterology and Nutrition. 52(3):307-313.
• Schiel, J., Park, K., Morphew, M.K., Reid, E., Hoenger, A., and Prekeris, R. (2011) Coordinated Endocytic Membrane Fusion and Buckling-Induced Microtubule Severing Mediate Cell Abscission. Journal of Cell Science. In Press.
• Schiel, J. and Prekeris, R. (2010) Making the final cut: Mechanisms mediating the abscission step of cytokinesis. The Scientific World Journal. 10:1424-1434.
• Hsu, VW and Prekeris, R. (2010) Mechanistic understanding of transport through the recycling endosome. Current Opinion in Cell Biology. 22(4):528-534.
• Willenborg, C., and Prekeris, R. (2011) Apical protein transport and lumen morphogenesis in polarized epithelial cells. Bioscience Reports. 31(4):245-256.
• Schiel, J., Park, K., Morphew, M.K., Reid, E., Hoenger, A., and Prekeris, R. (2011) Coordinated Endocytic Membrane Fusion and Buckling-Induced Microtubule Severing Mediate Cell Abscission. Journal of Cell Science. 124:1411-1424.
• Willenborg, C., Jing, J., Wu, C., Matern, H., Burden, J., and Prekeris, R. (2011) FIP5/Rip11 and SNX18 Interaction Regulates Epithelial Lumen Formation. The Journal of Cell Biology. 195(1):71-86.
• Jacob, A., Jing, J., Lee, J., Schedin, P., Peden, A.A., Junutula, J.R. and Prekeris, R. (2013) Rab40b regulates MMP2 and MMP9 trafficking during invadopodia formation and breast cancer cell invasion. Journal of Cell Science. 126:4647-4658.
• Maller, O., Hansen, K.C., Lyons, T.R., Acerbi, I.,Weaver, V.W., Prekeris, R., Tan, A.C., and Schedin, P. (2013) Collagen architecture in pregnancy-induced protection from breast cancer. Journal of Cell Science. 126:4108-4110.
• Jing, J., Hiu, L., O’Connor, B., Evans, C., Prekeris, R., Kobzik, L., Yang, I.V., and Schwartz, D.A. (2013) Role of macrophage receptor with collagenous structure in innate immunity tolerance. Journal of Immunology. 190(12):6360-6367.
• Willenborg, C., and Prekeris, R. (2011) Apical protein transport and lumen morphogenesis in polarized epithelial cells. Bioscience Reports. 31(4):245-256.
• Nachbar, J., Lazaro-Dieguez, F., Prekeris, R., Cohen, D. and Muesch, A. (2013) KifC3 promotes mitotic progression and integrity of the central spindle in cytokinesis. Cell Cycle. 13(3):426-433.
• Li, D., Mangan, A., Ciccini, L., Margolis, B., and Prekeris, R. FIP5 phosphorylation during mitosis regulates apical trafficking and luminogenesis. EMBO reports.
• Schiel, J., Childs, C., and Prekeris, R. (2013) Endocytic transport and cytokinesis: from regulation of the cytoskeleton to midbody inheritance. Trends in Cell Biology. 23(7):319-327.
• Schiel, J., and Prekeris, R. (2011) ESCRT or Endosomes? – Tales of the Separation of Two Daughter Cells. Communicative & Integrative Biology. 4(5):606-608.
• Prekeris, R. (2011) Actin Regulation During Abscission: Unexpected Roles of Rab35 and Endocytic Transport. Cell Research. 21(9):1283-1285.
• Prekeris, R. (2012) Making the final cut: the role of endosomes during mitotic cell division. Chapter in Book “Membrane Trafficking”. In Press.
• Arras, L., Yang, I., Lackford, B., Riches, D., Prekeris, R., Freedman, J.H., Schwartz, D.A., and Alper, S. (2012) Spatiotemporal inhibition of innate immunity signaling by the TBC1D23 Rab-GAP. The Journal of Immunology. 188(6):2905-2913.
• Li, D., Mangan, A., Ciccini, L., Margolis, B., and Prekeris, R. (2014) FIP5 phosphorylation during mitosis regulates apical trafficking and luminogenesis. EMBO reports. 15(4):428-437.
• Matsunami, N., Hensel, C.H., Baird, L., Stevens, J., Otterud, B., Leppert, T., Varvil, T., Hadley, D., Glessner, J., Pellegrino, R., Kim, C., Wang, T.F., Otieno, F.G., Ho, K., Christensen, B., Li, D., Prekeris, R., Lambert, C.G., Hakonarson, H., Leppert, M.F. (2014) Identification of rare DNA sequence variants in high-risk autism families and their prevalence in a large case/control population. Molecular Autism. 5(1):5.
• Ito, Y., Correll, K., Schiel, J., Finigan, J., Prekeris, R., and Mason, R. (2014) Lung Fibroblasts Accelerate Wound Closure in Primary Human Alveolar Epithelial Cells through Hepatocyte Growth Factor/c-Met signaling pathway. Am J Physiol Lung Cell Mol Physiol. 307(1):L94-105.
• Li, D., Kuehn, E.W., and Prekeris, R. (2014) Kinesin-2 mediates apical endosome transport during epithelial lumen formation. Cellular Logistics. 4(1):e28928.
• Collins, L.L., Simon, G.S., Matheson, J., Wu, C., Miller, M.C., Otani, T., Yu, X., Prekeris, R., and G.W. Gould (2012) Rab11-FIP3 is a cell cycle-regulated phosphoprotein. BMC Cell Biology. 13(1):4.
• Schiel, J.A., Simon, G., Castle, D., Christine C.W., D., and Prekeris, R. (2012) FIP3-endosome mediated actin depolymerization and formation of the secondary ingression mediates ESCRT-III recruitment to the abscission site during cytokinesis. Nature Cell Biology. 14(10):1068-1078.
• Mangan, A., and Prekeris, R. (2015) 3D-Time-Lapse analysis of Rab11/FIP5 complex: spatiotemporal dynamics during apical lumen formation. Methods in Molecular Biology. In Press.
• Prekeris, R. (2012) Making the final cut: the role of endosomes during mitotic cell division. Chapter in Book “Membrane Trafficking”.
• Jacob, A., and Prekeris, R. (2015) Regulation of MMP targeting to invadopodia during cancer metastasis. Frontiers in Cell and Developmental Biology. In Press.
• Prekeris, R. (2012) The art of “Cut and Run”: the role of Rab14 GTPase in regulating N-cadherin shedding and cell motility. Developmental Cell. 22(9):909-910.
• Prekeris R. Analyzing the functions of Rab11-effector proteins during cell division. Methods Cell Biol. 2015;130:19-34. PubMed PMID: 26360025
• Schiel, J., and Prekeris, R. (2012) Membrane dynamics during cytokinesis. Current Opinion in Cell Biology. 25(1):92-98.
• Schiel, J., Childs, C., and Prekeris, R. (2013) Endocytic transport and cytokinesis: from regulation of the cytoskeleton to midbody inheritance. Trends in Cell Biology. In Press.
• Blasky AJ, Mangan A, Prekeris R. Polarized Protein Transport and Lumen Formation During Epithelial Tissue Morphogenesis. Annu Rev Cell Dev Biol. 2015 Nov 13;31:575-91. PubMed PMID: 26359775
• Maller, O., Hansen, K., Lyons, T., Prekeris, R., Aik-Choon, T., and Schedin, P. (2013) Collagen topology in pregnancy-induced protection of mammary cancer. Journal of Cell Science. In Press.
• Jing, J., Hiu, L., O’Connor, B., Evans, C., Prekeris, R., Kobzik, L., Yang, I.V., and Schwartz, D.A. (2013) Role of MARCO in Innate Immune Tolerance. Journal of Immunology. In Press.
• Prekeris R. Cut or NoCut: the role of JADE1S in regulating abscission checkpoint. Cell Cycle. 2015;14(20):3219. PubMed PMID: 26327571
• Guo Y, Kenney SR, Muller CY, Adams S, Rutledge T, Romero E, Murray-Krezan C, Prekeris R, Sklar LA, Hudson LG, Wandinger-Ness A. R-Ketorolac Targets Cdc42 and Rac1 and Alters Ovarian Cancer Cell Behaviors Critical for Invasion and Metastasis. Mol Cancer Ther. 2015 Oct;14(10):2215-27. PubMed PMID: 26206334
• Song M, Giza J, Proenca CC, Jing D, Elliott M, Dincheva I, Shmelkov SV, Kim J, Schreiner R, Huang SH, Castrén E, Prekeris R, Hempstead BL, Chao MV, Dictenberg JB, Rafii S, Chen ZY, Rodriguez-Boulan E, Lee FS. Slitrk5 Mediates BDNF-Dependent TrkB Receptor Trafficking and Signaling. Dev Cell. 2015 Jun 22;33(6):690-702. PubMed PMID: 26004511
• Dionne LK, Wang XJ, Prekeris R. Midbody: from cellular junk to regulator of cell polarity and cell fate. Curr Opin Cell Biol. 2015 Aug;35:51-8. PubMed PMID: 25950842
• Mangan A, Prekeris R. 3D time-lapse analysis of Rab11/FIP5 complex: spatiotemporal dynamics during apical lumen formation. Methods Mol Biol. 2015;1298:181-6. PubMed PMID: 25800842
• Jacob A, Prekeris R. The regulation of MMP targeting to invadopodia during cancer metastasis. Front Cell Dev Biol. 2015;3:4. PubMed PMID: 25699257
• Jacob A, Linklater E, Bayless BA, Lyons T, Prekeris R. The role and regulation of Rab40b-Tks5 complex during invadopodia formation and cancer cell invasion. J Cell Sci. 2016 Dec 1;129(23):4341-4353. PubMed PMID: 27789576
• Mandell MA, Jain A, Kumar S, Castleman MJ, Anwar T, Eskelinen EL, Johansen T, Prekeris R, Deretic V. TRIM17 contributes to autophagy of midbodies while actively sparing other targets from degradation. J Cell Sci. 2016 Oct 1;129(19):3562-3573. PubMed PMID: 27562068
• Mangan AJ, Sietsema DV, Li D, Moore JK, Citi S, Prekeris R. Cingulin and actin mediate midbody-dependent apical lumen formation during polarization of epithelial cells. Nat Commun. 2016 Aug 3;7:12426. PubMed PMID: 27484926
• Dionne LK, Peterman E, Schiel J, Gibieža P, Skeberdis VA, Jimeno A, Wang XJ, Prekeris R. FYCO1 regulates accumulation of post-mitotic midbodies by mediating LC3-dependent midbody degradation. J Cell Sci. 2017 Dec 1;130(23):4051-4062. PubMed PMID: 29196475
• Gibieža P, Prekeris R. Rab GTPases and cell division. Small GTPases. 2017 May 4;:1-9. [Epub ahead of print] PubMed PMID: 28471300
• Peterman E, Prekeris R. Understanding post-mitotic roles of the midbody during cell differentiation and polarization. Methods Cell Biol. 2017;137:173-186. PubMed PMID: 28065304
• Linklater, E., Jewett, .E. and Prekeris, R. (2017) Polarized membrane trafficking in development and disease: from epithelia polarization to cancer cell invasion. In Press. Chapter in Book: “Cell Polarity in Development and Disease”.
• Antanaviciute I, Gibieža P, Prekeris R, Skeberdis VA. Midbody: From the Regulator of Cytokinesis to Postmitotic Signaling Organelle. Medicina (Kaunas). 2018 Jul 30;54(4). PubMed PMID: 30344284
• Jewett CE, Prekeris R. Insane in the apical membrane: Trafficking events mediating apicobasal epithelial polarity during tube morphogenesis. Traffic. 2018 May 16. [Epub ahead of print] PubMed PMID: 29766620
• Das L, Gard JMC, Prekeris R, Nagle RB, Morrissey C, Knudsen BS, Miranti CK, Cress AE. Novel Regulation of Integrin Trafficking by Rab11-FIP5 in Aggressive Prostate Cancer. Mol Cancer Res. 2018 Aug;16(8):1319-1331. PubMed PMID: 29759989
• Gibieža P, Prekeris R. Rab GTPases and cell division. Small GTPases. 2018 Mar 4;9(1-2):107-115. PubMed PMID: 28471300
• Peterman E, Prekeris R. The postmitotic midbody: Regulating polarity, stemness, and proliferation. J Cell Biol. 2019 Dec 2;218(12):3903-3911. PubMed PMID: 31690620
• Peterman E, Gibieža P, Schafer J, Skeberdis VA, Kaupinis A, Valius M, Heiligenstein X, Hurbain I, Raposo G, Prekeris R. The post-abscission midbody is an intracellular signaling organelle that regulates cell proliferation. Nat Commun. 2019 Jul 18;10(1):3181. PubMed PMID: 31320617
• Jewett CE, Appel BH, Prekeris R. The Rab11 effectors Fip5 and Fip1 regulate zebrafish intestinal development. Biol Open. 2020 Oct 23;9(10). PubMed PMID: 32973079
• Turn RE, East MP, Prekeris R, Kahn RA. The ARF GAP ELMOD2 acts with different GTPases to regulate centrosomal microtubule nucleation and cytokinesis. Mol Biol Cell. 2020 Aug 15;31(18):2070-2091. PubMed PMID: 32614697
• Peterman E, Valius M, Prekeris R. CLIC4 is a cytokinetic cleavage furrow protein that regulates cortical cytoskeleton stability during cell division. J Cell Sci. 2020 May 14;133(9). PubMed PMID: 32184265
• Duncan ED, Lencer E, Linklater E, Prekeris R. Methods to Study the Unique SOCS Box Domain of the Rab40 Small GTPase Subfamily. Methods Mol Biol. 2021;2293:163-179. PubMed PMID: 34453716
• Lencer E, Prekeris R, Artinger KB. Single-cell RNA analysis identifies pre-migratory neural crest cells expressing markers of differentiated derivatives. Elife. 2021 Aug 16;10. PubMed PMID: 34397384
• Linklater ES, Duncan ED, Han KJ, Kaupinis A, Valius M, Lyons TR, Prekeris R. Rab40-Cullin5 complex regulates EPLIN and actin cytoskeleton dynamics during cell migration. J Cell Biol. 2021 Jul 5;220(7). PubMed PMID: 33999101
• Gibieža P, Peterman E, Hoffman HK, Van Engeleburg S, Skeberdis VA, Prekeris R. Rab14/MACF2 complex regulates endosomal targeting during cytokinesis. Mol Biol Cell. 2021 Apr 1;32(7):554-566. PubMed PMID: 33566684
• Jewett CE, Soh AWJ, Lin CH, Lu Q, Lencer E, Westlake CJ, Pearson CG, Prekeris R. RAB19 Directs Cortical Remodeling and Membrane Growth for Primary Ciliogenesis. Dev Cell. 2021 Feb 8;56(3):325-340.e8. PubMed PMID: 33561422
• Hoffman HK, Prekeris R. Roles of the actin cytoskeleton in ciliogenesis. J Cell Sci. 2022 May 15;135(10). PubMed PMID: 35575063
• Han KJ, Mikalayeva V, Gerber SA, Kettenbach AN, Skeberdis VA, Prekeris R. Rab40c regulates focal adhesions and PP6 activity by controlling ANKRD28 ubiquitylation. Life Sci Alliance. 2022 Sep;5(9). PubMed PMID: 35512830
• McCurdy BL, Jewett CE, Stemm-Wolf AJ, Duc HN, Joshi M, Espinosa JM, Prekeris R, Pearson CG. Trisomy 21 increases microtubules and disrupts centriolar satellite localization. Mol Biol Cell. 2022 Jul 1;33(8). PubMed PMID: 35476505
• Duncan ED, Han KJ, Trout MA, Prekeris R. Ubiquitylation by Rab40b/Cul5 regulates Rap2 localization and activity during cell migration. J Cell Biol. 2022 Apr 4;221(4). PubMed PMID: 35293963
• Farmer T, Prekeris R. New signaling kid on the block: the role of the postmitotic midbody in polarity, stemness, and proliferation. Mol Biol Cell. 2022 Mar 1;33(3). PubMed PMID: 35179994
• Ceglowski J, Hoffman HK, Neumann AJ, Hoff KJ, McCurdy BL, Moore JK, Prekeris R. TTLL12 is required for primary ciliary axoneme formation in polarized epithelial cells. EMBO Rep. 2024 Jan;25(1):198-227. PubMed PMID: 38177908
• Farmer T, Vaeth KF, Han KJ, Goering R, Taliaferro MJ, Prekeris R. The role of midbody-associated mRNAs in regulating abscission. J Cell Biol. 2023 Dec 4;222(12). PubMed PMID: 37922419
• Neumann AJ, Prekeris R. A Rab-bit hole: Rab40 GTPases as new regulators of the actin cytoskeleton and cell migration. Front Cell Dev Biol. 2023;11:1268922. PubMed PMID: 37736498
• Hoffman HK, Prekeris R. HOPS-dependent lysosomal fusion controls Rab19 availability for ciliogenesis in polarized epithelial cells. J Cell Sci. 2024 Mar 1;137(5). PubMed PMID: 37665101
• Henen MA, Paukovich N, Prekeris R, Vögeli B. Solution NMR Backbone Assignment of the C-Terminal Region of Human Dynein Light Intermediate Chain 2 (LIC2-C) Unveils Structural Resemblance with Its Homologue LIC1-C. Magnetochemistry. 2023 Jul;9(7). PubMed PMID: 37476506
• Lencer E, Rains A, Binne E, Prekeris R, Artinger KB. Mutations in cdon and boc affect trunk neural crest cell migration and slow-twitch muscle development in zebrafish. Development. 2023 Jul 15;150(14). PubMed PMID: 37390228
• Jewett CE, McCurdy BL, O'Toole ET, Stemm-Wolf AJ, Given KS, Lin CH, Olsen V, Martin W, Reinholdt L, Espinosa JM, Sullivan KD, Macklin WB, Prekeris R, Pearson CG. Trisomy 21 induces pericentrosomal crowding delaying primary ciliogenesis and mouse cerebellar development. Elife. 2023 Jan 19;12. PubMed PMID: 36656118