Contents
A. Web Sites
B. Monoclonal Anti-Islet Antibodies
C. Hybridomas producing anti-GAD Monoclonal Antibodies
D. Autoantigen Plasmids
E. Autoantigens
F. Anti-Islet T Cell Clones
G. Tetramers
H. Animal Models – Mouse Transgenic/Knockout Models
I. Human Autoantibody Worskhops/Prociciency IDS/CDC
J. Genetic Resources
K. Human Pancreas

A. Web Sites
Barbara Davis Center for Childhood Diabetes: http://www.barbaradaviscenter.org
Two texts concerning type 1 diabetes, one on immunology of diabetes with teaching slides and the other (PinkPanther) with information for health care providers and families.

Immunology of Diabetes Society: http://www.idsoc.org
List members address and email, planned meetings, consensus statements, etc.

International Immunogenetics Database: http://imgt.cines.fr/
Integrated information system specializing in immunoglobulins, T cell receptors and Major histocompatiblity molecules of all vertebrate species.

HLA Informatics Group: http://www.anthonynolan.org.uk/HIG/
HLA sequence data and nomenclature.

National Center for Biotechnology Information: http://www.ncbi.nlm.nih.gov
Portal to GenBank, Pubmed, OMIM, genome sequence searches (Entrez and Blast), Taxonomy and Structure

Protein Data Bank: http://www.rcsb.org/pdb
Online Molecular structures

Juvenile Diabetes Foundation grants: http://www.jdrf.org/index.cfm?page_id=103227
Grant applications online

American Diabetes Association grants: http://www.diabetes.org/diabetes-research/research-grant-application-forms.jsp

Immune Tolerance Network: http://www.immunetolerance.org
Site for applications for trials and therapies relevant to induction of tolerance. Funding provided through grant to Jeff Bluestone from National Institutes of Health with non-U.S. funding allowed.

Children with Diabetes website: http://www.childrenwithdiabetes.com
Information and communication concerning childhood diabetes

Taconic Description of Genetics of NOD Mice: http://www.taconic.com
Under emerging models NOD

Type 1 Diabetes Resource: http://jaxmice.jax.org
Mouse and type 1 diabetes information resource website links from the Jackson Laboratory.

T1Dbase:    http://www.t1dbase.org/cgi-bin/welcome.cgi
A Bioinformatics Resource for Type 1 Diabetes Researchers with an integrated set of tools enables researchers to browser human type 1 diabetes susceptibility regions.

Bioinformatic Harvester   http://harvester.fzk.de/harvester/
Harvester caches and cross-links public bioinformatic databases and prediction servers to provide fast access to protein specific bioinformatic information. Presently the following databases and servers are implemented: SWALL, BLAST, SOURCE, SWALL, SMART, STRING, PSORT2, CDART, MapView and SOSUI.

Gene Expression Database: http://expression.gnf.org/cgi-bin/index.cgi
Tissue Expression Database Chip Type: Human U95A and Mouse U74A

Biology of Genes: http://genome-www5.stanford.edu/cgi-bin/source/sourceSearch
SOURCE is specifically designed to facilitate the analysis of large sets of data that biologists can now produce using genome-scale experimental approaches.

Endotext.org: http://www.endotext.org
Your Comprehensive, Authoritative, Updated, Endocrine Web-textbook

B. Monoclonal Anti-Islet Antibodies
1. Anti-human Insulin/C-peptide
c peptide MON 5021:
Anti-insulin MON5023:

Caltag Laboratories
436 Rozzi Place
South San Francisco, CA 94080

2. Monoclonal A2B5: (American Type Culture Collection)
ATCC CRL-1520

Islet and neuronal ganglioside (all vertebrates)
American Type Culture Collection
c/o Sales and Marketing Department
12301 Parklawn Dr.
Rockville, MD 20852
http://www.atcc.org

3. 3G5: ATCC CRL 1814 (American Type Culture Collection)
Pancreatic islet and neuronal ganglioside.

4. 4F2C13: ATCC HB-22 (American Type Culture Collection)
Heteromeic amino acid transporter (heavy chain) of human islet cell surface and proliferating cells (80:40 surface heterodimer).

C. Hybridomas Producing Anti-GAD Monoclonal Antibodies
Anti GAD-15:
Anti GAD-66:
Developmental Studies Hybridoma Bank
Dept. of Biology
University of Iowa
Iowa City, IA 57767

Anti GAD-15: ATCC HB 184
American Type Culture Collection
c/o Sales and Marketing Department
12301 Parklawn Dr.
Rockville, MD 20852

D. Autoantigen Plasmids
1. Human islet GAD65 Åke Lernmark, PhD

cDNA clone (pEx9): Robert H. Williams Laboratory
Department of Medicine, Box 357710
University of Washington
Seattle, WA 98195
ake@u.washington.edu

2. ICA69: George S. Eisenbarth, MD, PhD

Barbara Davis Center
1775 Aurora Ct.
BldgM20 MSB140
Aurora, CO 80045
george.eisenbarth@ucdenver.edu

3. ICA512bdc clone (AA 256-556/630-979 : George S. Eisenbarth, MD, PhD

Barbara Davis Center
1775 Aurora Ct.
BldgM20 MSB140
Aurora, CO 80045
george.eisenbarth@ucdenver.edu

4. IA-2 ic clone: Ezio Bonifacio

Intracytoplasmic domain of IA-2 (ICA512)
San Raffaele Institute, Milan
bonifacio.ezio@hsr.it

5. Phogrin (IA-2b): John Hutton

Barbara Davis Center
1775 Aurora Ct.
BldgM20 MSB140
Aurora, CO 80045
john.hutton@ucdenver.edu

6. IA-2 fragment clones: Liping Yu
A. Fragment 1: 761-964
B. Fragment 2A: 256-760
C. Fragment 2B: 761-928
D. Fragment 2C:929-797

Barbara Davis Center
1775 Aurora Ct.
BldgM20 MSB140
Aurora, CO 80045
liping.yu@ucdenver.edu

7. Human Transglutaminase Clone: George Eisenbarth

Barbara Davis Center
1775 Aurora Ct.
BldgM20 MSB140
Aurora, CO 80045
george.eisenbarth@ucdenver.edu

E. Autoantigens
1. GAD65 for T cell assays and Autoantibody Assays

Diamyd Medical, Sweden
http://www.diamyd.com

2. IA-2, human proinsulin, and mouse proinsulin II

John Elliott
University of Alberta
jell@maildrop.srv.ualberta.ca
(costs of production charged)

F. Anti-Islet T cell clones
BDC 2.5

Katie Haskins
University Of Colorado
katie.haskins@ucdenver.edu

Anti-Insulin (Mouse) Class II Restricted
BDC 6-4.2
BDC 4.1

John Hutton
Barbara Davis Center
1775 Aurora Ct.
BldgM20 MSB140
Aurora, CO 80045
john.hutton@ucdenver.edu

Anti-phogrin (IA-2 beta) T Cell clones
Nine I-A(g7) restricted phogrin Th1 murine clones with epitope specificity P2 and P7.

John Hutton
Barbara Davis Center
1775 Aurora Ct.
BldgM20 MSB140
Aurora, CO 80045
john.hutton@ucdenver.edu

Class I and Class II Restricted NOD T cell clones

Per Santamaria
University of Calgary
psantama@acs.ucalgary.ca

Anti-insulin (B:15-23) K(d) Restricted

Susan Wong
University of Bristol
susan.wong@bristol.ac.uk

G. Tetramers
1.National Institutes of Allergy and Infectious Disease

MHC Tetramer Core Facility
Emory University Vaccine Center
954 Gatewood Road
Atlanta, GA 30329
Phone: (404) 727-7215
http://research.yerkes.emory.edu/tetramer_core/index.html
http://www.niaid.nih.gov/reposit/tetramer/genguide.html
Source for class I tetramers, and potentially for class II tetramers.

2. Becton Coulter Tetramer (commerical)

Beckman Coulter, Inc.
Immunomics Operations
7330 Carroll Road
San Diego, CA 92121
Phone (866) 485-1270
immunomics@beckman.com

3. Jerry Nepom/Bill Kwok
Class II Human Tetramers

Virginia Mason Research Center
Seattle, WA
nepom@vmresearch.org
http://www.benaroyaresearch.org/research/quickresearch/tetramer/index.shtml

H. Animal Models- Mouse Transgenic/Knockout Models
1. NOD Mice, Multiple Strains (e.g. NOD Rag Mutation, NON, NOR)

Jackson Laboratories
http://jaxmice.jax.org

2. NOD mice: Taconic Farms, Inc.
“Wicker” Congenic Inbred Strains of NOD Mice

273 Hover Avenue
Germantown, NY 12526
http://www.taconic.com

3. NOD scid mice and multiple NOD strains: Edward H. Leiter, PhD

Jackson Laboratory
Bar Harbor, ME 04609
ehl@aretha.jax.org

4. BB rats: Dennis Guberski PhD

Biomedical Research Models, Inc
10 New Bond St.
Worcester, MA 01606
http://www.biomere.com

Islet with B7.1 (RIP[Rat Insulin Promoter])

Li Wen
Yale University

NOD Transgenic and Mutant Mouse Core

Ella Hyatt
Joslin Diabetes Center
Elzbieta.Hyatt@joslin.harvard.edu
http://citdh.org/mice_available.html

RIP-LCMV Mice

Matthias Von Herrath
Lajolla
matthias@liai.org

I. Human Autoantibody Workshops/Proficiency IDS/CDC
1. Human Diabetes Autoantibody Standardization Program (DASP): Proficieny and Workshop

Polly Bingley, Bristol University
polly.bingley@bristol.ac.uk

2. Mouse Autoantibody Workshop

Liping Yu and Clive Wasserfall (University Fl, Gainesville)
Barbara Davis Center for Childhood Diabetes
4200 East 9th Avenue, Box B140
Denver, CO 80262
liping.yu@ucdenver.edu

J. Genetic Resources
1. EBV cell lines/DNA from multiplex families with type I diabetes:

Human Biological Data Interchange
1880 JFK Blvd., 6th Fl
Philadephia, PA 19103
Phone (800) 345-4234
diabetes@hbdi.org
http://www.ndriresource.org/Biospecimens_Programs/HBDI/36/

British Diabetic Association

Warren Repository
10 Queen Anne Street
London, W1M 0BD
http://www.diabetes.org.uk/

2. Microsatellite markers:

Mouse and Human: Research Genetics
2130 Memorial Parkway SW
Huntsville, AL 35801

3. HLA typing:
DQA/DQB/DRB Typing Kits

Dynal
11 Bassendale Rd,
Croft Business Park
Bramborough, Wirral, CHE2, 3QL UK
http://www.tissue-typing.com

Luminex HLA Typing Kits
Lifematch HLA-DRB SSO Typing kit (cat# 628110)
Phone: 203-328-9500

K. Human Pancreas

National Disease Research Interchange
2401 Walnut Street
Suite 408
Philadelphia, PA 19104
diabetes@hbdi.org